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Anti-HP1 C1A9 is a monoclonal antibody produced by the Developmental Studies Hybridoma Bank. It is designed to detect heterochromatin protein 1 (HP1), a key regulator of chromatin structure and gene expression. The antibody specifically binds to the C-terminal domain of HP1.

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Lab products found in correlation

Anti ar n 20, by Santa Cruz Biotechnology (1 mentions) Anti flag m2 or rabbit, by Merck Group (1 mentions) Anti mdc1, by Abcam (1 mentions) Anti ar441, by Thermo Fisher Scientific (1 mentions) Anti gcn5, by Santa Cruz Biotechnology (1 mentions) Epifluorescence microscope, by Zeiss (1 mentions) Confocal microscope, by Zeiss (1 mentions)

2 protocols using anti hp1 c1a9

1

Antibody Panel for Epigenetic Study

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The antibodies used in this study were: anti-Flag (M2 or rabbit, Sigma), anti-trimethyl H3-K9, anti-AcH3, anti-AcH4, anti-trimethyl H3-K27, anti-AcH4-K16, anti-AcH3-K9 (Upstate Biotechnology), anti-HP1 C1A9 (Developmental Studies Hybridoma Bank at the University of Iowa), anti-GCN5, anti-AR (N-20) (Santa Cruz Biotechnology), anti-MDC1 (Abcam), anti-MYST1 (Bethyl laboratories), anti-AR 441, anti-p21WAF1 Ab-11 (Thermo scientific), anti-GAPDH (Shanghai Kangchen).
Wang C., Sun H., Zou R., Zhou T., Wang S., Sun S., Tong C., Luo H., Li Y., Li Z., Wang E., Chen Y., Cao L., Li F, & Zhao Y. (2015). MDC1 functionally identified as an androgen receptor co-activator participates in suppression of prostate cancer. Nucleic Acids Research, 43(10), 4893-4908.
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2

Immunostaining of Drosophila Germline

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Mouse monoclonal anti-HP1 (C1A9; 1:50), anti-FasIII (7G10; 1:200; for hub cells), anti-α-spectrin (3A9; 1:10; for fusome) were from Developmental Studies Hybridoma Bank (University of Iowa). Rabbit antibodies against histone H3 (1:1000), H3K4me3 (1:500), and H3K9me3 (1:250) were from Upstate Biotechnology. These antibodies and rabbit anti-Vasa (1:1000; generous gifts from Ruth Lehmann) were used as primary antibodies and fluorescent (Molecular Probes) or HRP conjugated secondary antibodies were used in whole-mount immunostaining or Western blotting, respectively. Tissues were fixed in 4% paraformaldehyde and 0.3% Triton-X. Stained tissues were photographed with a Leica confocal microscope or a Zeiss epifluorescence microscope. Images were cropped and minimally processed with Adobe Photoshop.
Xing Y, & Li W.X. (2015). Heterochromatin components in germline stem cell maintenance. Scientific Reports, 5, 17463.
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