Malme 3

Human cells were obtained from American Type Culture Collection (ATCC) (Manassas, VA) and stored in vials immersed in N₂(l). Prior to their use, human cell lines were authenticated by morphology, karyotyping, and PCR-based methods, which included an assay to detect species specific variants of the cytochrome C oxidase I gene (to rule out interspecies contamination) and short tandem repeat profiling (to distinguish between individual human cell lines and rule out intraspecies contamination). To minimize genetic drift, a thawed vial was used for fewer than fifteen passages.
Medium and added components, trypsin (0.25% w/v), and Dulbecco’s phosphate-buffered saline (PBS) were from the Gibco^® brand from Thermo Fisher Scientific (Waltham, MA). Cells were grown in flat-bottomed culture flasks in a cell-culture incubator at 37 °C under CO₂(g) (5% v/v). A549 cells (ATCC CCL-185) were grown in F-12K medium; H358 (ATCC CRL-5807) cells were grown in RPMI-1640 medium; SK-MEL-28 cells (ATCC HTB-72) were grown in Eagle’s minimum essential medium; A375 cells (ATCC CRL-1619) and HEK293T cells (ATCC CRL-1573) were grown in Dulbecco’s modified Eagle’s medium; Malme-3M (ATCC HBT-64) cells were grown in Iscove’s modified Dulbecco’s medium; Malme-3 (ATCC HTB-102) cells were grown in McCoy’s 5a modified medium. The Corning 96-well microplates used in experiments were from Sigma–Aldrich.