The cDNAs for wild-type SKN-1, truncated SKN-1 peptides, and PRMT-1 of C. elegans were amplified by PCR and cloned into pGEX-6P-1. All of the point mutations of SKN-1 were generated by site-directed mutagenesis according to the QuikChange (Stratagene) protocol. The antibodies of anti-GFP, anti-GST (Tianjin Sungene Biotech), anti-β-actin (Sigma), anti-ASYM24 (Millipore) and anti-PRMT1 (Upstate) were purchased from the commercial channels.
Anti gst
Manufactured by Sungene Biotech
Anti-GST is a laboratory reagent designed for the detection and purification of proteins tagged with Glutathione S-Transferase (GST). It is a recombinant protein that binds to GST with high affinity, enabling the isolation and identification of GST-tagged proteins.
Automatically generated - may contain errors
2 protocols using anti gst
1
Cloning and Mutation of C. elegans SKN-1 and PRMT-1
2
Cloning and Modification of C. elegans SKN-1 and OGT-1
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The cDNAs for skn-1, skn-1(321–623aa) and ogt-1 of C. elegans were amplified by PCR and cloned into pGEX-6P-1 and PET-28a vectors, respectively. All of the point mutations of SKN-1 were generated by site-directed mutagenesis according to the QuikChange (Stratagene) protocal. The antibodies of anti-GFP, anti-GST (Tianjin Sungene Biotech), anti-β-actin (Sigma), anti-O-GlcNAc RL2 (Abcam), anti-OGT (sigma) and anti- phosphoserine (Millipore) were purchased from the commercial channels. The anti-OGT and anti-GFP antibodies were first analyzed and make sure of their specific binding to the OGT-1 and GFP in C. elegans (Supplementary Fig. S10a,b ). Rabbit polyclonal antibodies against phospho-SKN-1(Ser483) were generated by using synthetic peptides, TTDSSSTCS(#)RLSSESPRYTSE. # means the phosphorylated residue Ser483.
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