Innotest phospho tau 181p kit

For AD core biomarkers, we used the V-PLEX Aβ Peptide Panel 1 (6E10) Kit (K15200E) and the V-PLEX Human Total Tau Kit (K151LAE) (Mesoscale Diagnostics LLC, Rockville, MD, USA), as well as the INNOTEST PHOSPHO TAU(181P) kit (81581, Fujirebio, Ghent, Belgium). For inflammation-associated proteins, detailed information is provided in Additional file 1.
In the initial evaluative step, all inflammatory markers were classified as detectable, undetectable, or borderline. The last group contained markers at detection limits for which test results suggested that addition of a known dose of protein to the sample could be used to improve the assay signal (spike-in concept). A full record of this test phase is provided in Additional file 1.
CSF samples saved strictly for research purposes had been snap-frozen in liquid nitrogen and stored in a biobank at −80 °C. Prior to assay, samples were thawed on ice and divided into aliquots (total of two freeze-thaw cycles). All further sample processing was conducted on ice until samples were applied to the assays. Samples and calibrators were run in duplicates, and samples with a coefficient of variation (CV) > 20% were repeated. To normalize for interrun variance, a pooled and aliquoted CSF sample was run as an internal standard on each assay plate.