Biomark real time pcr analysis software v2

Manufactured by Standard BioTools

BioMark Real-Time PCR Analysis Software v2.0 is a data analysis software designed for use with the BioMark HD System. It provides tools for the analysis of real-time PCR data generated by the BioMark HD System.

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Lab products found in correlation

Taqman microrna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) 2100 bioanalyzer, by Agilent Technologies (1 mentions)

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Real-Time PCR Analysis software (82 citations) BioMark Real-Time PCR Analysis software (16 citations) BioMark Real-time PCR Analysis (13 citations) Real-Time PCR Analysis (6 citations) BioMark PCR analysis software (2 citations)

2 protocols using biomark real time pcr analysis software v2

Quantifying Inflammation-Related miRNAs

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This study is based in the expression of 96 miRNAs. These miRNAs have been carefully selected based on those predicted by bioinformatic algorithms and databases to regulate genes known to be relevant to inflammation. Total RNA will be extracted from cryopreserved samples using Paxgene Blood miRNA kit (PreAnalitiX) according to the manufacturer’s protocol. RNA integrity will be tested using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California, US). Samples will be reverse transcribed using the Taqman^®MicroRNA Reverse Transcription Kit (Applied Biosystems, Life Technologies, Carlsbad, California, US) and mature miRNAs will be quantified by real-time PCR using the Taqman^®MicroRNA Assays [26 (link)]. miRNA expression will be analyzed using the BioMark 96:96 Dynamic Array (Fluidigm Corporation, San Francisco, California, US). Data will be analyzed using the BioMark Real-Time PCR Analysis Software v2.0 (Fluidigm). The Ct value will be used for analysis by the 2^-∆∆Ct method [27 (link)].

Marin J.M., Artal J., Martin T., Carrizo S.J., Andres M., Martin-Burriel I., Bolea R., Sanz A., Varona L., Godino J., Gallego B., Garcia-Erce J.A., Villar I., Gil V., Forner M., Cubero J.P, & Ros L. (2014). Epigenetics modifications and Subclinical Atherosclerosis in Obstructive Sleep Apnea: The EPIOSA study. BMC Pulmonary Medicine, 14, 114.

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Real-Time qPCR Analysis of HSPCs

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HSPCs were directly sorted into reaction buffer. AML GFP⁺ cells were sorted into Trizol. We performed RT and real-time quantitative PCR with SYBR Green PCR Master Mix using designed primers. All reactions were carried out at least in triplicate. In each experiment, cell populations were sorted from three individual mice. Data were analyzed using BioMark Real-Time PCR Analysis Software v.2.0 (Fluidigm). The results from each experiment were normalized to the expression of glyceraldehyde-3-phosphate dehydrogenase. The primers used for the amplification of the indicated genes are listed in table S2.

Sun Y., Lin X., Liu B., Zhang Y., Li W., Zhang S., He F., Tian H., Zhu X., Liu X., Wu J., Cai J, & Li M. (2021). Loss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells. Science Advances, 7(52), eabj6877.

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