The human iPS cell line 201B was provided by Dr. Shinya Yamanaka (Department of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan). 201B cells were maintained in tissue culture plates coated with recombinant human truncated vitronectin (Invitrogen, Carlsbad, CA) in Essential 8 medium (Invitrogen) supplemented with 10 μM ROCK inhibitor Y27632 (Invitrogen). Cells were routinely passaged as small clumps using the EDTA method with the split ratio of 1:8 to 1:12 every 2 to 3 days after reaching 60% to 80% confluence [31 (link)]. The human iPSC line ChiPS17 was purchased from TaKaRa (Shiga, Japan) and cultured using Cellartis DEF-CS Culture System (TaKaRa) according to the manufacturer’s instructions. Human fibroblast BJ and cancer cell lines (K562, HeLa and HEK293) were purchased from the Health Science Research Resources Bank (Osaka, Japan) and maintained in RPMI1640 or DMEM medium (Sigma-Aldrich, St. Louis, MO) supplemented with 10% heat-inactivated fetal calf serum (FCS) (Sigma-Aldrich).
Hek293
Manufactured by Health Science Research Resources Bank
Sourced in Japan
HEK293 is a cell line derived from human embryonic kidney cells. It is a commonly used cell line in research due to its ease of culture and transfection. HEK293 cells are capable of expressing a wide variety of proteins and are often used as a model system for studying protein function, signaling pathways, and gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Essential 8 medium, by Thermo Fisher Scientific (1 mentions)
Cellartis def cs culture system, by Takara Bio (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Dld 1, by Health Science Research Resources Bank (1 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)
E myco valid mycoplasma pcr detection kit, by iNtRON Biotechnology (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Fetal calf serum (fcs), by American Type Culture Collection (1 mentions)
Roswell park memorial institute (rpmi), by American Type Culture Collection (1 mentions)
Met 5a, by American Type Culture Collection (1 mentions)
3 protocols using hek293
1
Maintenance of Human iPSC Lines
2
Cell Line Authentication and Mycoplasma Screening
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The human embryonic kidney cell line HEK293 and the human colorectal cancer cell line DLD-1 were obtained from the Health Science Research Resources Bank (Osaka, Japan) and maintained in DMEM (Thermo Fisher Scientific) and RPMI (Thermo Fisher Scientific) supplemented with 10% fetal calf serum (Thermo Fisher Scientific), respectively. The human colorectal cancer cell line HCT116 was purchased from the American Type Culture Collection and cultured in RPMI supplemented with 10% fetal calf serum. These cell lines are not listed in the International Cell Line Authentication Committee database of cross-contaminated or misidentified cell lines. Absence of mycoplasma contamination was routinely confirmed using the e-Myco VALiD Mycoplasma PCR Detection Kit (iNtRon Biotechnology). All cell lines were authenticated by short tandem repeat profiling.
3
Comprehensive Molecular Profiling of Malignant Mesothelioma
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The 19 MM cell lines used in this study and their suppliers are listed in Supplementary Table 1 (Supplemental Digital Content 1, http://links.lww.com/JTO/A805). An immortalized mesothelial cell line, MeT-5A, was purchased from the American Type Culture Collection (Manassas, VA). The human embryonic kidney cell line HEK293 was obtained from the Health Science Research Resources Bank (Osaka, Japan). The cell lines were maintained in accordance with the suppliers' recommendations.
The clinical and pathological characteristics of 23 patients whose fresh frozen tissue samples were analyzed by NGS are summarized in Supplementary Table 2 (Supplemental Digital Content 1, http://links.lww.com/JTO/A805). Formalinfixed paraffin-embedded tissue sections were collected from 44 MM patients (including the aforementioned 23 patients) who underwent panpleuropneumonectomy or pleural biopsy at the National Cancer Center (NCC) Hospital (Tokyo, Japan) between 1995 and 2012 with written informed consent. This study was conducted with approval from the Institutional Review Board of the National Cancer Center.
The clinical and pathological characteristics of 23 patients whose fresh frozen tissue samples were analyzed by NGS are summarized in Supplementary Table 2 (Supplemental Digital Content 1, http://links.lww.com/JTO/A805). Formalinfixed paraffin-embedded tissue sections were collected from 44 MM patients (including the aforementioned 23 patients) who underwent panpleuropneumonectomy or pleural biopsy at the National Cancer Center (NCC) Hospital (Tokyo, Japan) between 1995 and 2012 with written informed consent. This study was conducted with approval from the Institutional Review Board of the National Cancer Center.
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