22 gauge catheter

Manufactured by BD

The 22-gauge catheter is a medical device designed for intravenous (IV) access. It is a hollow, thin tube made of flexible material that is inserted into a vein, typically in the arm or hand, to allow for the administration of fluids, medications, or other treatments directly into the bloodstream. The 22-gauge size refers to the diameter of the catheter.

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Lab products found in correlation

L9143, by Merck Group (1 mentions) Inveon ct scanner, by Siemens (1 mentions)

4 protocols using 22 gauge catheter

Aerosolized LPS Exposure in Mice

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Mice were exposed to aerosolized LPS (from P. aeruginosa 10, Sigma L9143) using a nebulizer (Pulmo-Aide compressor) as previously described (75 (link)). LPS (12.5 mg) dissolved in 5.0 mL of PBS were administered using a nebulizer connected to a container with vent holes. Five milliliters of solution were administered over 15 min.
At 24 h after nebulization, mice were anesthetized. To distinguish between circulating and lung resident or infiltrating human immune cells, fluorochrome labeled (FITC or APC/Cy7) anti-human CD45 antibody (2.5 μg, clone HI30) was intravenously injected. Mice were kept under anesthesia for at least 5 min after injection. Blood samples were collected retro-orbitally and BAL was performed using standard methods with a 22-gauge catheter (BD Biosciences) (76 ). Lungs were then aspirated with sterile PBS. Mice were then killed for bone marrow and lung collection. All tissues were processed, stained, and analyzed as previously described.

Zheng Y., Sefik E., Astle J., Karatepe K., Öz H.H., Solis A.G., Jackson R., Luo H.R., Bruscia E.M., Halene S., Shan L, & Flavell R.A. (2022). Human neutrophil development and functionality are enabled in a humanized mouse model. Proceedings of the National Academy of Sciences of the United States of America, 119(43), e2121077119.

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Visualizing Spleen Vascular Changes in Parasite Infection

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To study the spleen vascular changes upon parasite infection, spleen casts were prepared. Mice were euthanized with preinjection of heparin. A 22-gauge catheter (BD) was surgically inserted into the descending aorta. The spleen was first perfused with 1× PBS followed by complete Mercox II solution (Ladd Research Industries) injected through the catheter. Mice were left overnight for Mercox II polymerization and then were immersed into 10% NaOH for tissue digestion for a period of 10 to 14 days. Spleen samples were washed with distilled water, and spleen casts were air dried and collected for analysis.
The spleen casts were scanned using an Inveon CT scanner (Siemens). The scan was acquired with 2-by-2 binning at an exposure time of 7,800 ms per projection. A total of 1,200 projections for high-resolution and high-magnification image scans were performed. The acquired images were assembled using Inveon software and then processed, reconstructed, and visualized using MatLab. The vessel complexities were analyzed using FIJI software (49 (link)). Diameters of splenic veins and splenic arteries at the points where they connect with the spleen were measured, and the numbers of venous branches were quantified.

Huang X., Huang S., Ong L.C., Lim J.C., Hurst R.J., Mushunje A.T., Matsudaira P.T., Han J, & Preiser P.R. (2015). Differential Spleen Remodeling Associated with Different Levels of Parasite Virulence Controls Disease Outcome in Malaria Parasite Infections. mSphere, 1(1), e00018-15.

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Murine Model of LPS-Induced Lung Inflammation

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LPS-induced lung inflammation was investigated on Kunming mice according to protocols reported previously (Ferretti et al., 2003 (link); Bohr et al., 2017 (link)). 35 mice were randomly divided into five groups, seven mice per group. Mice were dosed intranasally with 10 mg/kg of ART, mPEG_2k-ART, mPEG_5k-ART, mPEG_2k-SS-ART, mPEG_2k-SS-ART (1 µL drug solution/g of mice weight), or saline alone, respectively. 1 h later, the mice were nasal administered with LPS solution (1.25 mg/kg) using a pipet by pacing the mice on their backs and allowing them for inhalation of the solution through the nostrils (1 µL LPS solution/g of mice weight). 0.9% NaCl solution was administered in the saline group. The mice were euthanized 6 h later via an over dose of pentobarbital (i.p. injection of 200 μL of pentobarbital solution, 20 mg/ml) for bronchoalveolar lavage (BAL), and the trachea was exposed from an incision and cannulated with a 22-gauge catheter (BD biosciences). Subsequently, BAL was performed by flushing the lungs with 1.0 ml PBS for 2 times and was used for protein quantification and cell counting/differentiation. The BAL was centrifuged at 2 k rpm for 10 min at 4°C, the supernatant was stored at −80°C for protein quantification, and the pellets were pooled and resuspended in fresh PBS.

Hao D.L., Wang Y.J., Yang J.Y., Xie R., Jia L.Y., Cheng J.T., Ma H., Tian J.X., Guo S.S., Liu T., Sui F., Zhao Y., Chen Y.J, & Zhao Q.H. (2022). The Alleviation of LPS-Induced Murine Acute Lung Injury by GSH-Mediated PEGylated Artesunate Prodrugs. Frontiers in Pharmacology, 13, 860492.

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Acute LPS-induced Lung Inflammation in Mice

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A widely used protocol for acute LPS-induced lung inflammation [28] [29] [30] was adjusted for CD-1 mice.
The mice were anesthetized by intraperitoneal (i.p.) injection of 200 µl Ketamine (3.5 mg/ml)/Xylazine solution (5 mg/ml), and the animals were monitored until disappearance of the plantar reflex. Using a pipette, the LPS solution (3.2 mg/kg) was administered to the mice by nasal administration (1.2 µl LPS solution/g of mouse weight) by placing the mice on their backs and allowing them to inhale the solution through the nostrils. Subsequently, the mice were allowed to wake up and were monitored over the next days. For bronchoalveolar lavage (BAL), the mice were euthanized via an overdose of pentobarbital (i.p. injection of 200 µl 20 mg/ml pentobarbital solution), and the trachea was exposed from an incision and cannulated with a 22-gauge catheter (BD biosciences). Subsequently, BAL was performed by flushing the lungs with seven times 0.3 ml PBS, and the first two lavages (collected separately) were used for protein quantification and cell counting/differentiation, whereas the last five lavages were used only for cell counting/differentiation. The BAL was centrifuged at 400g for 10 min, the supernatant was stored at -20 °C for protein quantification, and the pellets were pooled and resuspended in fresh PBS.

Bohr A., Tsapis N., Andreana I., Chamarat A., Foged C., Delomenie C., Noiray M., El Brahmi N., Majoral J.P., Mignani S, & Fattal E. (2017). Anti-Inflammatory Effect of Anti-TNF-α SiRNA Cationic Phosphorus Dendrimer Nanocomplexes Administered Intranasally in a Murine Acute Lung Injury Model. Biomacromolecules, 18(8).

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