Fluorshield with dapi

Manufactured by Merck Group

Sourced in United States

Fluorshield with DAPI is a mounting medium used in fluorescence microscopy. It contains the fluorescent dye DAPI, which binds to DNA and emits blue fluorescence when excited. This product is used to mount and preserve fluorescently labeled samples for microscopic analysis.

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Lab products found in correlation

Axiovert 200m, by Zeiss (4 mentions) Proteinase k, by Merck Group (2 mentions) Lsm 880, by Zeiss (2 mentions) Zen 2, by Zeiss (1 mentions) Proteostat, by Enzo Life Sciences (1 mentions) Elyra ps 1, by Zeiss (1 mentions) Alexafluor594 labeled goat anti mouse, by Thermo Fisher Scientific (1 mentions)

4 protocols using fluorshield with dapi

Intracellular Fluorescence Imaging of HCT116 Cells

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Example 4

Cell Culture and Fluorescence Imaging: Confocal Laser Fluorescence Microscopic Images of Hct116 Cells.

Hct116 cells (3×10⁵) (procured from national repository of National Centre for Cell Science, Ganeshkind Road, Pune: 411007, Maharashtra, India) were seeded on coverslips placed in 6 well plates. After 24 hours cells were treated with R (10 μM) for 30 minutes or pre-treated with N-Ethyl Maleimide (NEM, a thiol specific blocking reagent (1 mM) for 30 minutes before adding R (10 μM) for 30 minutes. Cells were then washed thrice with Phosphate Buffer Saline (1×PBS) and fixed with 4% PFA for 20 minutes and washed again with 1×PBS. Permeabilization of the cells was done using 0.2% Triton X 100 for 5 minutes. Again three washes were given and then coverslips mounted using Fluor shield with DAPI (Sigma) mounting medium. Nail paints was used to seal the coverslips mounted on the glass slides. Images were acquired in Olympus Fluoview Microscope. Overlay of the merged images confirms the intracellular fluorescence. (FIG. 11)

US10155776B2. Method for selective detection and estimation of histidine and cystein (2018-12-18). Council of Scientific and Industrial Research [IN]. Inventors: Amitava Das [IN], Samit Chattopadhyay [IN], Upendar Reddy Gandra [IN], Hridesh Agarwalla [IN].

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Protein Aggregation Detection in MEFs

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MEFs were cultivated on glass coverslips (Laboratory Glassware Marienfeld). 24 h after seeding, cells were heat stressed at 42 °C for 1 h or treated with 0.5 µM MG-132 for 48 h or 100 nM BafA1 for 48 h. Cells were fixed for 15 min with 4 % paraformaldehyde in PBS pH 7.4 and permeabilized with 0.5% (v/v) Triton X-100, 3 mM EDTA pH 8.0, and 5% goat serum in PBS for 30 min at room temperature and then stained with Proteostat® (Enzo Life Sciences, Inc.) at a dilution of 1:2000 in 1× assay buffer (ENZO) for 20 min. Cells were then mounted in Fluorshield with DAPI (Sigma). Fluorescence microscopy was performed using a Zeiss ELYRA PS.1 equipped with an LSM880 (Carl Zeiss, Oberkochen) with a 63 × oil immersion objective. Super-resolution images were generated by the Structured Illumination (SIM) technology. SIM confocal images were processed using the ZEN2.1 software (Carl Zeiss, Jena).

Furthmann N., Bader V., Angersbach L., Blusch A., Goel S., Sánchez-Vicente A., Krause L.J., Chaban S.A., Grover P., Trinkaus V.A., van Well E.M., Jaugstetter M., Tschulik K., Damgaard R.B., Saft C., Ellrichmann G., Gold R., Koch A., Englert B., Westenberger A., Klein C., Jungbluth L., Sachse C., Behrends C., Glatzel M., Hartl F.U., Nakamura K., Christine C.W., Huang E.J., Tatzelt J, & Winklhofer K.F. (2023). NEMO reshapes the α-Synuclein aggregate interface and acts as an autophagy adapter by co-condensation with p62. Nature Communications, 14, 8368.

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Immunofluorescent Detection of Collagen II in Intervertebral Discs

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Collagen type II (COL2) distribution in the IVD was analyzed by immunofluorescence staining. Antigen retrieval was performed in paraffin sections through incubation with 20 μg/mL proteinase K (Sigma-Aldrich) solution for 15 min at 37 °C. Sections were incubated overnight, at 4 °C, with anti-collagen II-II6B3 (1:20, Developmental Studies Hybridoma Bank at the University of Iowa, Department of Biology, Iowa City, IA, USA) antibody, followed by incubation with Alexa Fluor 594-labeled goat anti-mouse (1:1000, Invitrogen) antibody. Sections were mounted in Fluorshield with DAPI (Sigma-Aldrich). Representative images of the slides were taken using fluorescence microscopy (Axiovert 200 M, Zeiss, Oberkochen, Germany). The percentage of COL2 in NP was determined as described by Cunha et al. [11 (link)].

Cunha C., Q. Teixeira G., Ribeiro-Machado C., L. Pereira C., Ferreira J.R., Molinos M., G. Santos S., Barbosa M.A, & M. Goncalves R. (2020). Modulation of the In Vivo Inflammatory Response by Pro- Versus Anti-Inflammatory Intervertebral Disc Treatments. International Journal of Molecular Sciences, 21(5), 1730.

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Collagen Type II Immunofluorescence Staining in IVD

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Collagen type II (Col II) distribution in the IVD was analysed by immunofluorescence staining. Antigen retrieval was performed in paraffin sections through incubation with 20 μg/mL proteinase K (Sigma-Aldrich Inc., St Louis, MO, USA) solution for 15 min at 37 °C. Sections were incubated overnight at 4 °C with the anti-collagen II-II6B3 (Developmental Studies Hybridoma Bank, 1:20 dilution) antibody followed by incubation with Alexa Fluor 594-labeled goat anti-mouse antibody (1:1000, Molecular Probes, Invitrogen, Carlsbad, CA, USA). Sections were mounted in Fluorshield with DAPI (Sigma-Aldrich Inc., St Louis, MO, USA). Representative images of the slides were collected using an inverted fluorescence microscope (Axiovert 200 M, Zeiss, Jena, Germany). The %COL2 in the NP was determined as previously described [20 (link)].

Cunha C., Leite Pereira C., Ferreira J.R., Ribeiro-Machado C., Grad S., Santos S.G, & Gonçalves R.M. (2021). Therapeutic Strategies for IVD Regeneration through Hyaluronan/SDF-1-Based Hydrogel and Intravenous Administration of MSCs. International Journal of Molecular Sciences, 22(17), 9609.

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