Interleukin 1 α il1 α

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Interleukin 1-α (IL1-α) is a cytokine involved in the regulation of immune and inflammatory responses. It is a member of the interleukin 1 family of proteins. IL1-α plays a role in the activation of various target cells.

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Lab products found in correlation

Tumor necrosis factor α tnf α, by Thermo Fisher Scientific (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Amicon ultra 15 3 kda, by Merck Group (1 mentions) Tumor necrosis factor α tnf α, by R&D Systems (1 mentions) Interferon γ ifn γ, by Merck Group (1 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions) Interferon γ ifn γ, by Thermo Fisher Scientific (1 mentions) X vivo 20 media, by Lonza (1 mentions)

3 protocols using interleukin 1 α il1 α

Hypoxic Preconditioning of hMSCs

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When either hMSCs or mBMSCs (P2 passage in culture) reached 80% of confluence, they were rinsed three times in PBS to remove any residue of FBS. The cells were then replenished with a serum-free (SF) medium (D-MEM for hMSCs or alpha-MEM for mBMSCs) supplemented with 2 mM of L-glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin, without FBS, for 24 h.
For the preconditioning of the hMSCs, the SF medium was discarded, and the cells were cultured in the SF medium supplemented with inflammatory stimuli (50 ng/mL Tumor Necrosis Factor-α (TNF-α) (PeproTech, London, UK) and 50 ng/mL Interleukin 1-α (IL1-α) (PeproTech, London, UK) under hypoxic condition (1% O₂) (Hyp^INF) for 24 h.
Subsequently, the CM was collected from both cell sources and concentrated with Amicon-Ultra-15, 3 kDa centrifugal filter tubes (Merck, Darmstadt, Germany). In order to quantify the protein amount, a BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) was used. The CM samples were then stored at −80 °C and used in experiments as 50 μg.

Gorgun C., Africano C., Ciferri M.C., Bertola N., Reverberi D., Quarto R., Ravera S, & Tasso R. (2022). Preconditioned Mesenchymal Stromal Cell-Derived Extracellular Vesicles (EVs) Counteract Inflammaging. Cells, 11(22), 3695.

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Induction of iNOS Expression

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To induce iNOS expression, subconfluent monolayers were cultured in serum-free medium for 24 h. Growth-arrested cultures were treated with pro-inflammatory cytokines, 100 U/ml interferon γ (IFN-γ) (Sigma-Aldrich, St. Louis, MO, USA), 10 ng/ml interleukin-1 α (IL-1α) (PeproTech, Inc., Rocky Hill, NJ, USA) and 25 ng/ml tumor necrosis factor-α (TNF-α) (R&D Systems, Minneapolis, MN, USA), pro-inflammatory cytokines and 0.1–5 mg/ml water extract of Cnidii Rhizoma or 0.5 mM 1400W (Sigma-Aldrich) in fresh medium without fetal bovine serum. After 48 h, the supernatants were collected and the cells were harvested and lysed as previously described (18 (link)).

NAM K.S., HA B.G, & SHON Y.H. (2014). Effect of Cnidii Rhizoma on nitric oxide production and invasion of human colorectal adenocarcinoma HT-29 cells. Oncology Letters, 9(1), 483-487.

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Expansion of Cytokine-Induced Killer Cells

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The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). The study was approved by the Human Research Ethics committee of Lanzhou University Second Hospital (ethics approval number: 2017A-044). Informed consent was obtained from all individual participants included in the study. PBMCs were obtained using Ficoll-Hypaque density centrifugation. Cells were resuspended at 1×10⁶ CFU/mL in X-VIVO-20 media (Lonza, Basel, Switzerland) with 1,000 U/mL interferon-γ (IFN-γ, Peprotech, Rocky Hill, NJ, USA) for the first 24 h. On day 1, 50 ng/mL anti-CD3 monoclonal antibody (OKT3, Peprotech, Rocky Hill, NJ, USA), 100 U/mL interleukin-1α (IL-1α, Peprotech, Rocky Hill, NJ, USA) and IL-2 (Peprotech, Rocky Hill, NJ, USA) were added to the medium. Cells were cultured in 5% CO₂ at 37 °C, and fresh medium and IL-2 were added every two days. The concentration of IL-2 was 300, 500, and 1,000 U/mL in the first week; and 300, 500, 1,000, 3,000, and 6,000 U/mL in the second week, respectively. CIKs were harvested on day 16.

Liu Y., Li J., Zhao L., Zhu J., Liu S., Wang H, & Zhang Y. (2021). Effects of interleukin-2 concentration and administration method on proliferation and function of cytokine-induced killer cells. Translational Cancer Research, 10(9), 3930-3938.

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