Larval tissues were stained with standard immunohistochemical procedures using rabbit anti-cleaved PARP antibody (Cell Signaling Technology, CST, 1:200), mouse anti-Wg antibody (Developmental Studies Hybridoma Bank (DSHB), 1:500), rabbit anti-phospho-histone H3 (Ser10) antibody (CST, 1:100), rabbit anti-β-galactosidase antibody (MP Biomedicals, 1:2000), mouse anti-β-galactosidase antibody (Sigma, 1:500), and rabbit anti-Cleaved Drosophila Dcp-1 (Asp216) antibody (CST, 1:100) for 3 days. For EdU labeling, larvae were dissected in Schneider’s Drosophila medium (GIBCO) containing 5% FBS and incubated in the same media with 12.5 μM EdU for 30 min. Detection of EdU was performed with Click-iT Plus EdU Alexa Fluor 647 Imaging Kit (Thermo Fisher Scientific; C10640), according to the manufacture’s instruction. Samples were mounted with DAPI-containing SlowFade Gold Antifade Reagent (Molecular Probes). Images were taken with a Leica SP5, SP8, or SPE confocal microscope.
Rabbit anti cleaved parp antibody
Manufactured by Cell Signaling Technology
Rabbit anti-cleaved PARP antibody is a lab equipment product that detects the cleaved form of Poly(ADP-ribose) polymerase (PARP), a protein involved in the detection and repair of DNA damage. This antibody can be used to identify and quantify the cleaved PARP protein, which is commonly used as a marker for apoptosis or programmed cell death.
Automatically generated - may contain errors
Lab products found in correlation
Slowfade gold antifade reagent, by Thermo Fisher Scientific (2 mentions)
Mouse anti wg antibody, by Developmental Studies Hybridoma Bank (2 mentions)
Schneider s drosophila medium, by Thermo Fisher Scientific (1 mentions)
Spe confocal microscope, by Leica (1 mentions)
Click it plus edu alexa fluor 647 imaging kit, by Thermo Fisher Scientific (1 mentions)
Rabbit anti cleaved dcp 1, by Cell Signaling Technology (1 mentions)
Sp5 confocal microscope, by Leica (1 mentions)
2 protocols using rabbit anti cleaved parp antibody
1
Immunohistochemical Staining and EdU Labeling
2
Immunostaining of Larval Tissues
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Larval tissues were stained with standard immunohistochemical procedures using rabbit anti-cleaved PARP antibody (Cell Signaling Technology, CST, 1:200), rabbit anti-cleaved Dcp1 (Cell Signaling, 1:100 for 3 days), mouse anti-Wg antibody (Developmental Studies Hybridoma Bank, DSHB, 1:500) and mounted with 4, 6-diamidino-2-phenylindole (DAPI)containing SlowFade Gold antifade reagent (Molecular Probes). Images were taken with a Leica SP5 confocal microscope.
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