Ab17725

Manufactured by Abcam

Ab17725 is a monoclonal antibody that specifically binds to the alpha subunit of the NaK-ATPase enzyme. The core function of this product is to serve as a tool for the detection and analysis of the NaK-ATPase protein in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Fc82291, by Merck Group (1 mentions) Fibronectin, by Merck Group (1 mentions) Axio observer z1 microscope, by Zeiss (1 mentions) Dnmt1, by Novus Biologicals (1 mentions) Nb100 56519, by Novus Biologicals (1 mentions) A4700, by Merck Group (1 mentions) Anti mouse alexa flour 594, by Thermo Fisher Scientific (1 mentions) Mouse anti brdu, by BD (1 mentions) Nb100 182, by Novus Biologicals (1 mentions) H 300, by Santa Cruz Biotechnology (1 mentions) M1445, by Merck Group (1 mentions) Ab92312, by Abcam (1 mentions) Ab1790, by Abcam (1 mentions) Ab57447, by Abcam (1 mentions) Ab290, by Abcam (1 mentions) D5c7p, by Cell Signaling Technology (1 mentions) Ab16048, by Abcam (1 mentions)

4 protocols using ab17725

PLA Assay for Protein-Protein Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

The PLA assay was preformed according to the manufacturer’s instructions (OLINK Bioscience). In brief, cells were plated on 12 mm coverslips pre-coated with 10 μg ml⁻¹ fibronectin (Sigma) and fixed with 3% paraformaldehyde. When indicated, cells were ultraviolet irradiated with 10 J m⁻² at time points before fixation. Permeabilization was done with 0.1% Triton X-100. Coverslips were blocked and then probed with rabbit anti polη (AB17725, Abcam, diluted 1:500) and mouse anti-NPM1 (FC82291, Sigma, diluted 1:500). Anti-rabbit-plus and anti-mouse-minus PLA probes were added, ligated and amplified according to the manufacturer’s instructions. Immunostaining of NPM1 was done using anti-NPM1 (FC82291, Sigma, 1:500). Images were captured with Zeiss Axio-Observer Z1 microscope and analysed with CellProfiller software68 (link).

Ziv O., Zeisel A., Mirlas-Neisberg N., Swain U., Nevo R., Ben-Chetrit N., Martelli M.P., Rossi R., Schiesser S., Canman C.E., Carell T., Geacintov N.E., Falini B., Domany E, & Livneh Z. (2014). Identification of novel DNA-damage tolerance genes reveals regulation of translesion DNA synthesis by nucleophosmin. Nature Communications, 5, 5437.

+ Open protocol

+ Expand

Western Blot Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were used for western blotting mouse anti-actin (A4700, Sigma Aldrich), PCNA (PC10, Millipore), rabbit anti-Cdt1 (D10F11, Cell Signalling Technology), DNMT1 (NB100-56519, Novus Biologicals), Fen1 (EPR4459(2), GeneTex), Ligase I (PA5-27820, Thermo Scientific), p21 (EPR362, abcam), Polη (ab17725, abcam), Ubiquitinated-PCNA (D5C7P, Cell Signalling Technology).

Wilson R.H., Biasutto A.J., Wang L., Fischer R., Baple E.L., Crosby A.H., Mancini E.J, & Green C.M. (2017). PCNA dependent cellular activities tolerate dramatic perturbations in PCNA client interactions. DNA Repair, 50, 22-35.

+ Open protocol

+ Expand

Antibody Characterization for DNA Damage

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used in this study: anti-SPRTN (rabbit, polyclonal) raised against the N-terminal part (1–240 aa) of SPRTN (dilution 1:1,000, home made); anti-SPRTN raised against the C-terminal part of SPRTN (dilution 1:1,000, Atlas, HPA 025073); anti–DNA polymerase η (dilution 1:1,000, Abcam, Ab17725); anti–phosphorylated γ-H2AX (Ser139) (dilution 1:300, Millipore, 05-636); anti-zebrafish γ-H2AX (dilution 1:1,000, gift of J. Amatruda); anti-rabbit 53BP1 (dilution 1:200, Santa Cruz, sc-22760); anti–Ki-67 (dilution 1:200, Millipore, MAB 4190); anti-rat BrdU (dilution 1:500, Abcam, 6326); anti-mouse BrdU (dilution 1:100, Becton Dickinson, 347850); anti-mouse Alexa Flour 488 (dilution 1:300, Invitrogen, A21202), anti-mouse Alexa Flour 594 (dilution 1:300, Invitrogen, A11020); anti-mouse horseradish peroxidase (HRP) (dilution 1:10,000, Sigma, A2304); and anti-rabbit HRP (1:10,000, Sigma, A0545).

Lessel D., Vaz B., Halder S., Lockhart P.J., Marinovic-Terzic I., Lopez-Mosqueda J., Philipp M., Sim J.C., Smith K.R., Oehler J., Cabrera E., Freire R., Pope K., Nahid A., Norris F., Leventer R.J., Delatycki M.B., Barbi G., von Ameln S., Högel J., Degoricija M., Fertig R., Burkhalter M.D., Hofmann K., Thiele H., Altmüller J., Nürnberg G., Nürnberg P., Bahlo M., Martin G.M., Aalfs C.M., Oshima J., Terzic J., Amor D.J., Dikic I., Ramadan K, & Kubisch C. (2014). Mutations in SPRTN cause early onset hepatocellular carcinoma, genomic instability and progeroid features. Nature genetics, 46(11), 1239-1244.

+ Open protocol

+ Expand

Antibody Detection Protocol for DNA Repair Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies to the following proteins were used in this study: sheep α-FAN1 (1:500, a kind gift from John Rouse, University of Dundee), rabbit α-FANCD2 (1:5000, NB100-182, Novus Biologicals), mouse α-PCNA (1:1000, PC10, SantaCruz), rabbit α-ubiquitylated-PCNA (1:1000, D5C7P, Cell Signaling), mouse α-RAD18 (1:500, ab57447, Abcam), rabbit α-MLH1 (1:5000, ab92312, Abcam), rabbit α-Polη (1:1000, ab17725, Abcam), rabbit α-REV1 (1:1000, H-300, SantaCruz), rabbit α-GFP (1:1000, ab290, Abcam), rabbit α-LaminB1 (1:1000, ab16048, Abcam), rabbit α-histone H2B (1:1000, ab1790, Abcam), mouse α−MUS81 (1:1000, M1445, Sigma Aldrich), rabbit α−USP1 (1:1000, Bethyl labs. A301-700A).

Porro A., Berti M., Pizzolato J., Bologna S., Kaden S., Saxer A., Ma Y., Nagasawa K., Sartori A.A, & Jiricny J. (2017). FAN1 interaction with ubiquitylated PCNA alleviates replication stress and preserves genomic integrity independently of BRCA2. Nature Communications, 8, 1073.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!