Log In Sign up
The largest database of trusted experimental protocols

Anti cd161 pe clone hp 3g10 and anti il 17 alexa488 clone ebio64dec17

Manufactured by Thermo Fisher Scientific
Visit Supplier

Anti-CD161-PE (clone HP-3G10) is a fluorochrome-conjugated monoclonal antibody that binds to the CD161 cell surface marker. Anti-IL-17-Alexa488 (clone eBio64DEC17) is a fluorochrome-conjugated monoclonal antibody that binds to the IL-17 cytokine.

Automatically generated - may contain errors

Lab products found in correlation

Aqua live dead amine dye amcyan, by Thermo Fisher Scientific (2 mentions) Anti cd8 qdot705 clone 3b5, by Thermo Fisher Scientific (2 mentions) Anti cxcr3 alexafluor 488 clone 1c6 cxcr3, by BD (2 mentions) Anti cd28 ecd clone cd28.2, by Beckman Coulter (2 mentions) Anti cd161 bv421 clone hp 3g10, by BioLegend (2 mentions)

Close spelling variants of this product

Anti-IL-17-alexa488 Anti-IL-17-PE Anti-CD161-PE IL-17-PE Anti-IL-17 EBio64DEC17 IL-17

2 protocols using anti cd161 pe clone hp 3g10 and anti il 17 alexa488 clone ebio64dec17

1

Comprehensive Immune Profiling by Flow Cytometry

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Fourteen-parameter flow cytometric analysis was performed on peripheral blood, LN, and RB derived cells according to standard procedures using a panel of monoclonal antibodies that we and others have shown to be cross-reactive with SMs and RMs.12 (link) The following antibodies were used at predetermined optimal concentrations: anti-CCR6-PE-Cy7 (clone 11A9), anti-CCR5-PE and -APC (clone 3A9), anti-CD3-APC-Cy7 (clone SP34-2), anti-CD62L-FITC (clone SK11), anti-CD95-PE-Cy5 (clone DX2), anti-Ki-67-Alexa700 (clone B56), anti-IFN-γ-Alexa700 (clone B27), and anti-CXCR3-AlexaFluor 488 (clone 1C6/CXCR3) from BD Biosciences; anti-CD161-PE (clone HP-3G10) and anti-IL-17-Alexa488 (clone eBio64DEC17) from eBioscience; anti-CD28-ECD (clone CD28.2) from Beckman Coulter; anti-CD4-BV421 (clone OKT4), anti-CD4-BV605 (clone OKT4), and anti-CD161-BV421 (clone HP-3G10) from Biolegend; anti-CD8-Qdot705 (clone 3B5) and Aqua Live/Dead amine dye-AmCyan from Invitrogen. Flow cytometric acquisition was performed on at least 100,000 CD3+ T cells on an LSRII cytometer driven by the FACS DiVa software, or at least 10,000 CD3+ T cells for rectal biopsy-derived cells. The data acquired were analyzed using FlowJo software (version 9.8.5; TreeStar).
McGary C.S., Alvarez X., Harrington S., Cervasi B., Ryan E.S., Iriele R.I., Paganini S., Harper J., Easley K., Silvestri G., Ansari A.A., Lichterfeld M., Micci L, & Paiardini M. (2017). The loss of CCR6+ and CD161+ CD4+ T cell homeostasis contributes to disease progression in SIV-infected rhesus macaques. Mucosal immunology, 10(4), 1082-1096.
+ Open protocol
+ Expand
2

Comprehensive Immune Profiling by Flow Cytometry

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Fourteen-parameter flow cytometric analysis was performed on peripheral blood, LN, and RB derived cells according to standard procedures using a panel of monoclonal antibodies that we and others have shown to be cross-reactive with SMs and RMs.12 (link) The following antibodies were used at predetermined optimal concentrations: anti-CCR6-PE-Cy7 (clone 11A9), anti-CCR5-PE and -APC (clone 3A9), anti-CD3-APC-Cy7 (clone SP34-2), anti-CD62L-FITC (clone SK11), anti-CD95-PE-Cy5 (clone DX2), anti-Ki-67-Alexa700 (clone B56), anti-IFN-γ-Alexa700 (clone B27), and anti-CXCR3-AlexaFluor 488 (clone 1C6/CXCR3) from BD Biosciences; anti-CD161-PE (clone HP-3G10) and anti-IL-17-Alexa488 (clone eBio64DEC17) from eBioscience; anti-CD28-ECD (clone CD28.2) from Beckman Coulter; anti-CD4-BV421 (clone OKT4), anti-CD4-BV605 (clone OKT4), and anti-CD161-BV421 (clone HP-3G10) from Biolegend; anti-CD8-Qdot705 (clone 3B5) and Aqua Live/Dead amine dye-AmCyan from Invitrogen. Flow cytometric acquisition was performed on at least 100,000 CD3+ T cells on an LSRII cytometer driven by the FACS DiVa software, or at least 10,000 CD3+ T cells for rectal biopsy-derived cells. The data acquired were analyzed using FlowJo software (version 9.8.5; TreeStar).
McGary C.S., Alvarez X., Harrington S., Cervasi B., Ryan E.S., Iriele R.I., Paganini S., Harper J., Easley K., Silvestri G., Ansari A.A., Lichterfeld M., Micci L, & Paiardini M. (2017). The loss of CCR6+ and CD161+ CD4+ T cell homeostasis contributes to disease progression in SIV-infected rhesus macaques. Mucosal immunology, 10(4), 1082-1096.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!