Kanamycin

Graphene quantum dots (GQDs, 6–9 nm diameter, 0.8–1.2 nm thickness) and tungsten disulfide nanosheets (WS₂, 20–500 nm diameter, 1–8 nm thickness) were obtained from Nanjing XFNANO Materials Tech Co. Ltd. (Nanjing, China). Malachite green, leuco malachite green (LMG), and 6-mercapto-1-hexanol (MCH) was purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Chloramphenicol, kanamycin, gentamicin sulfate, and methylene blue were received from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The thiolated MG binding aptamer was purchased from Shanghai Sangon Biotech Co. Ltd. (Shanghai, China), the base sequences are as follows 5’-SH-GGA UCC CGA CUG GCG AGA GCC AGG UAA CGA AUG GAU CC-3’. Tris-EDTA buffer (TE, 10 mM, pH 7.4) was used to prepare stock solution of aptamer. The phosphate buffer (PB, 0.067 M) at diverse pH was prepared by Na₂HPO₄ and KH₂PO₄.

Wild-type A. rhizogenes A4 agropine-type strain obtained from Agricultural Culture Collection of China (ACCC) and its transformant containing the binary plasmid pBI121 (14.7 Kb) were used in this study. pBI121 contains uidA reporter gene (GUS, β-glucuronidase) driven by the cauliflower mosaic virus 35S promoter and the terminator of nopaline synthase (nos) gene. The binary vector was introduced into A. rhizogenes strains by electroporation [34 (link)] and transformants were selected on A4 solid media (10 g sucrose, 0.2 g MgSO₄.7H₂O, 0.5 g K₂HPO₄, 0.2 g CaSO₄, 0.1 g NaCl, 1.0 ml of 1% NaMoO₄, 1 ml of 1% C₆H₅FeO₇, 1 ml of 1% Boric acid, 1.0 g Yeast extract and 15 g Agar in 1000 ml of double distilled H₂O, pH 6.8–7.0) (Sinopharm Chemical Reagent Co. Ltd, Shanghai, China) supplemented with 50 mg/l kanamycin.

NheI and SpeI were obtained from New England Biolabs. Nickel nitrilotiacetic acid resin was obtained from Qiagen. 1-dodecanethiol (DT, 98%), diethyldithiocarbamic acid silver salt (98%), trichlormethane, acetone, dimethyl sulfoxide (DMSO), ampicillin, and kanamycin were purchased from Sinopharm Group Chemical Reagent Co. Ltd. Chlorin e6 (Ce6) was obtained from Frontier Scientific Inc., and 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[amine methoxy (polyethylene glycol)₂₀₀₀] (DSPE-mPEG₂₀₀₀-NH₂) was purchased from Avanti. Ultrapure water (Millipore Mill-Q grade, 18.2 MΩ) was used in all the experiments. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS) were obtained from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). DMEM/F12 and RPMI 1640 were purchased from Thermo Fisher Scientific. Staphylococcus aureus’ species-identifiable aptamer was obtained from Tsingke Biotechnology Co., Ltd. All reagents and solvents are of analytical grade and used without further purification.