All solvents were LC-MS grade. Water and isopropyl alcohol were purchased from Honeywell Burdick & Jackson (Muskegon, Michigan); chloroform, acetonitrile, methanol, acetic acid, low retention microcentrifuge tubes, serological pipettes were purchased from Fisher Scientific (Fair Lawn, New Jersey); plastic pipette tips were purchased from USA Scientific (Orlando, Florida); methyl tert-butyl ether was purchased from J.T. Baker (Central City, Pennsylvania); internal standards were purchased from Avanti Polar lipids Inc. and Sigma Aldrich (St. Louis, MO); pyrex glass culture tubes were purchased from Corning Incorporated (Corning, New York).
Serological pipettes
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom
Serological pipettes are laboratory equipment used to accurately measure and transfer specific volumes of liquids, typically in the range of 1 to 100 milliliters. They feature graduated markings along the length of the pipette to allow for precise volume measurement.
Automatically generated - may contain errors
Lab products found in correlation
Isopropyl alcohol, by Avantor (2 mentions)
Low retention microcentrifuge tube, by Thermo Fisher Scientific (2 mentions)
Plastic pipette tips, by USA Scientific (2 mentions)
Acetic acid, by Thermo Fisher Scientific (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Chloroform, by Thermo Fisher Scientific (2 mentions)
Pyrex glass culture tubes, by Corning (2 mentions)
Methyl tert butyl ether (mtbe), by Avantor (2 mentions)
Internal standards, by Avanti Polar Lipids (2 mentions)
50 ml centrifuge tube, by Thermo Fisher Scientific (2 mentions)
Thioanisole, by Merck Group (1 mentions)
Anisole, by Merck Group (1 mentions)
Diethyl ether, by Thermo Fisher Scientific (1 mentions)
Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt, by Merck Group (1 mentions)
Pl rink resin, by Polymer Laboratories (1 mentions)
Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions)
1 8 diazabicyclo 5.4.0 undec 7 ene dbu, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
6 protocols using serological pipettes
1
Lipid Extraction and Quantification Protocol
2
Analytical Lipid Extraction Workflow
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All solvents were LC-MS grade. Water and isopropyl alcohol were purchased from Honeywell Burdick & Jackson (Muskegon, MI, USA); chloroform, acetonitrile, methanol, acetic acid, low retention microcentrifuge tubes, serological pipettes were purchased from Fisher Scientific (Fair Lawn, NJ, USA); plastic pipette tips were purchased from USA Scientific (Orlando, FL, USA); methyl tert-butyl ether was purchased from J.T. Baker (Central City, PA, USA); internal standards were purchased from Avanti Polar lipids Inc. and Sigma Aldrich (St. Louis, MO, USA); pyrex glass culture tubes were purchased from Corning Incorporated (Corning, NY, USA).
3
Peptide Synthesis for Cell Assays
4
Cytotoxicity of Zinc Dithiocarbamate Complex
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zinc diethyldithiocarbamate (Zn (DDC)2) (molecular weight of 361.99 g/mole and >99.0% purity) was purchased from Tokyo Chemical Industry (TCI) Co., Ltd., Tokyo, Japan. 2-hydroxypropyl-beta-cyclodextrin of USP grade (HP) (molecular weight: 1555 g/mole) and Sulfobutyl ether beta-cyclodextrin sodium (SBE) (molecular weight: 2242.05 g/mole) were bought from Glentham Wiltshire, UK. MD. Acetonitrile laboratory of reagent grade > 99% (molecular weight: 41.05 g/mole) was purchased from Fisher Scientific, Loughborough, UK. Lung cancer cell lines A549 were obtained from ATCC (Teddington, UK). Dulbecco’s modified Eagle’s medium (DMEM), Gibco™ foetal bovine serum (FBS), penicillin streptomycin (Pen-Strep) antibiotic solution, non-essential amino acid solution and L-glutamine (cell culture-tested, 99.0–101.0%), 50 mL centrifuge tubes (sterile), tissue culture flask of 75 cm2 (sterile), serological pipettes (sterile), and Gibco™ Trypsin-EDTA (0.25%), were purchased from Fisher Scientific, Loughborough, UK. 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) and Dimethyl sulfoxide (DMSO) were purchased from Glentham Life Science, Wiltshire, UK. Corning® 96-well TC-treated microplates were obtained from Sigma-Aldrich, Dorset, UK.
5
Mononuclear Cell Isolation from ALS Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh peripheral blood from ALS patients and healthy volunteers was collected in sterile tubes with heparin (10 units of heparin per 1 ml of blood; BD, Franklin Lakes, NJ, USA) and was then diluted (1:1) with sterile phosphatebuffered saline (PBS) without Mg 2+ and Ca 2+ (Sigma-Aldrich, St. Louis, MO, USA) at 37°C. Then 12.5 ml of Ficoll (Histopaque-1077, Cat. No.10771; Sigma-Aldrich) was added into 50-ml sterile centrifuge tubes (Cat. No. 352074; BD Falcon, Bedford, MA, USA). Blood samples diluted in PBS were overlaid on the Ficoll and centrifuged at 400 × g for 40 min at 26°C. The MNC layer was transferred with plasma to new 50-ml tubes by using 10-ml serological pipettes Fisherbrand, Waltham, MA, USA) . The MNCs were washed twice in 30 ml of PBS at 440 × g for 13 min at 21°C. The cell numbers and viability were determined using a Vi-CELL Viability Analyzer (Beckman Coulter, Brea, CA, USA). The MNCs were frozen in Cryopreservation Media (Saneron CCEL Therapeutics, Inc. Tampa, FL, USA) at 2 × 10 6 cells per vial and stored in liquid nitrogen.
6
Preparation and Evaluation of Lipid Nanocarriers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Soya phosphatidylcholine (SPC; Lipoid S-100), hydrogenated phosphatidylcholine (HSPC; Phospholipon 90H) and dipalmitoyl phosphatidylcholine (DPPC) were obtained from Lipoid, Switzerland. EMEM (Eagle's minimum essential medium), non-essential amino acid solution and L-glutamine (cell culture tested, 99.0 -101.0 %) were purchased from Lonza, Switzerland. Trypsin-EDTA solution, ethanol (absolute and 70%), 96-well plates (sterile with lids), 50 ml centrifuge tubes (sterile), tissue culture flask 75 cm 2 (sterile) and serological pipettes (sterile) were obtained from Fisher Scientific, UK. Cholesterol (Chol; ≥ 99%), glass vials (15 ml), poly-L-lysine (PLL) hydrobromide (molecular weight 30,000-70,000), dextran (molecular weight 5,000 approx.), Dimethyl sulfoxide (DMSO), thiazolyl blue tetrazolium bromide, Fetal bovine serum (FBS), Phosphate buffered saline (PBS) tablets, Trypan blue solution (0.4% liquid, sterile filtered), Syringe filters (0.2 and 0.45 µm), syringe needles and sterile pipette tip boxes were all purchased from Sigma Aldrich, UK. Human glioblastome cell line (U87-MG) and Human glial cell line (SVG-P12) were obtained from European collection of cell cultures, UK. Paclitaxel (PX) was purchased from ChemieTek, USA.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!