Magnisort negative selection beads

Manufactured by Thermo Fisher Scientific

Magnisort Negative selection beads are paramagnetic beads designed for the isolation of specific cell populations from complex biological samples. The core function of these beads is to facilitate the depletion of unwanted cells, allowing for the enrichment of the target cell population. The beads are coated with antibodies that bind to the surface markers of the unwanted cells, enabling their magnetic separation from the sample.

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Lab products found in correlation

Celltrace violet, by Thermo Fisher Scientific (2 mentions) Celltrace yellow, by Thermo Fisher Scientific (1 mentions) Facsaria sorter, by BD (1 mentions) Anti apc magnetic beads, by Miltenyi Biotec (1 mentions)

2 protocols using magnisort negative selection beads

T Cell Isolation and Labeling

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T cells were isolated by negative selection using biotinylated antibodies against CD11b, CD11c, CD19, CD25, NK1.1, TCRγΔ, and Ter119 and Magnisort Negative selection beads (Thermo Fisher Scientific) according to the manufacturer’s instructions, then labeled with CellTraceViolet or CellTraceYellow (Thermo Fisher Scientific). Dyes were swapped between genotypes between experiments. 1 × 10⁶ to 2 × 10⁶ T cells per group were transferred via retro-orbital injection.

Dixit D., Hallisey V.M., Zhu E.Y., Okuniewska M., Cadwell K., Chipuk J.E., Axelrad J.E, & Schwab S.R. (2024). S1PR1 inhibition induces proapoptotic signaling in T cells and limits humoral responses within lymph nodes. The Journal of Clinical Investigation, 134(4), e174984.

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Adoptive transfer of alveolar macrophages

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C57BL/6 (n=10) and B6.SJL (n=1 or 2) mice were infected with H37Rv Mtb-mCherry by aerosol. On day 15 post-infection, C57BL/6 mice were intratracheally administered 0.25 μg SiglecF PE antibody 30 min prior to euthanasia. Single-cell lung suspensions were prepared, and CD19⁺ cells were depleted with Magnisort negative selection beads (Thermo). Airway⁺ alveolar macrophages were sorted based on SiglecF PE expression and autofluorescence on a FACSAria sorter (BD) in a BSL-3 facility. Sorted cells were then adoptively transferred intratracheally into infection-matched or naive B6.SJL mice. Due to cell yield limitations, alveolar macrophages from ten donor mice were transferred into only one or two recipients. Five days post-transfer, recipient B6.SJL mice were intratracheally administered 0.25 μg CD11c BV650 antibody, and lungs were harvested 30 min later. Transferred alveolar macrophages were enriched by CD45.2 positive selection pull-downs using anti-APC magnetic beads and LS columns (Miltenyi Biotec) followed by flow cytometric analysis of airway labeling.

Cohen S.B., Gern B.H., Delahaye J.L., Adams K.N., Plumlee C.R., Winkler J., Sherman D.R., Gerner M.Y, & Urdahl K.B. (2018). Alveolar macrophages provide an early Mycobacterium tuberculosis niche and initiate dissemination. Cell host & microbe, 24(3), 439-446.e4.

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