High glucose Dulbecco’s Modified Eagle Medium (DMEM), trypsin and penicillin–streptomycin, were obtained from Macgene (Beijing, China). Fetal bovine serum (FBS) was from PAN-Biotech (Aidenbach, Germany). Complete/Incomplete Freund’s adjuvant and LPS were from Sigma-Aldrich (St Louis, MO, USA). Bovine type II collagen was from Macklin (Shanghai, China). Methotrexate was obtained from Bidepharm (Shanghai, China). 4% paraformaldehyde was from Bioroyee Biotechnology (Beijing, China). EDTA and DAPI were from Solarbio (Beijing, China). Goat anti-rabbit secondary antibody, endogenous peroxidases blocker and goat serum albumin were from ZSGB-Biotech ((Beijing, China). PMA are purchased from Med Chem Express (MCE). Hoechst 33342 was obtained from TargetMol (Shanghai, China). Sytox Green was from Beyotime (Shanghai, China). Primary antibody of IL-6 was from Bioss (Beijing, China). Primary antibody of TNF-α was from Proteintech (Chicago, IL, USA). Primary antibodies against PI3K/p-PI3K were from Bioworld (St. Louis Park, MN, USA). Primary antibodies against p-Akt and p-mTOR were bought from Cell Signaling Technology (Beverly, MA, USA). Primary antibody against Akt was from Biogot (Nanjing, China). Antibodies of mTOR, MPO, CitH3 were from Abcam (Cambridge, UK).
Methotrexate
Manufactured by Bide Pharmatech
Sourced in China
Methotrexate is a chemical compound used in various laboratory applications. It functions as a folate antagonist, inhibiting the enzyme dihydrofolate reductase. This mechanism is central to its use in a variety of research and analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Beyotime (2 mentions)
Sytox green, by Beyotime (1 mentions)
Hoechst 33342, by Targetmol (1 mentions)
Freund s complete incomplete adjuvant, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Fetal bovine serum (fbs), by PAN Biotech (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Solarbio (1 mentions)
Penicillin streptomycin, by Macgene (1 mentions)
Ab259341, by Abcam (1 mentions)
Ab215188, by Abcam (1 mentions)
Ab178945, by Abcam (1 mentions)
Ab7291, by Abcam (1 mentions)
F4 80, by Bioss Antibodies (1 mentions)
Cy5.5 nhs ester, by Apexbio (1 mentions)
Cd206, by Abcam (1 mentions)
Matrigel, by Corning (1 mentions)
Trifluoroacetic acid tfa, by Energy Chemical (1 mentions)
1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride, by Merck Group (1 mentions)
Hyaluronic acid, by Bide Pharmatech (1 mentions)
3 protocols using methotrexate
1
Immunomodulatory Agents in Inflammatory Conditions
2
Fluorescent Peptide Synthesis and Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Amino acids, 2-(naphthalene-2-yl) acetic acid, and O-(benzotriazole-1-yl)-N,N,N,N-tetramethyluronium hexafluorophosphate (HBTU) were purchased from Bidepharm (Shanghai, China). 1-Pyrenemethylamine hydrochloride and trifluoroacetic acid (TFA) were obtained from Energy Chemical (Beijing, China). 2-Cl-Trityl chloride resin was bought from Nankai Resin Co. Ltd (Tianjin, China). N,N-Diisopropylethylamine (DIEA) was purchased from J & K (Beijing, China). Glycine, phenylalanine, tyrosine, and methotrexate were bought from Bidepharm (Shanghai, China). Cy5.5 NHS ester was obtained from APExBIO (USA). ELISA kit for IL-6 was bought from MSKBIO (China). Matrigel was bought from Corning (USA). Tubulin, iNOS, IL-6, and TNF-α antibodies used in western blot were purchased from Abcam (ab7291, ab178945, ab259341, and ab215188, respectively). In immunofluorescence staining, F4/80 was obtained from Bioss (bsm-34028 M), iNOs was obtained from Proteintech (18985-1-AP), CD206 was obtained from Abcam (ab64693), CD31 was brought from Servicebio (GB11063-2). DAPI was obtained from Beyotime Biotechnology (Shanghai, China). Commercially available reagents were used without further purification.
3
Methotrexate-Hyaluronic Acid Conjugate for Skin Cancer Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methotrexate (MTX, purity ≥99%), hyaluronic acid (HA, 8–12 kDa), hyaluronic acid (HA, 100–200 kDa), 1-hydroxypyrrolidine-2,5-dione (NHS, purity ≥98%) and cysteamine hydrochloride were purchased from Bide Pharmatech Ltd. (Shanghai, China). 4M hydrogen chloride solution in 1,4-dioxane was purchased from Infinity Scientific Co. Ltd. (Beijing, China). 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI), 4-dimethylaminopyridine (DMAP), polylactic acid (PLA, 5000 Da), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and fluorescein isothiocyanate (FITC) were obtained from Sigma-Aldrich Co. Ltd. (St. Louis, MO, USA). Dialysis tubing (500–1000 Da, 3500 Da) was purchased from Merck Chemicals Shanghai Co., Ltd (Shanghai, China). HaCaT cell line and fetal bovine serum (FBS) were purchased from Nanjing KeyGEN Biotech Co., Ltd. (Nanjing, China). DMEM medium, trypsin, and penicillin–streptomycin solution were purchased from Fcmacs Biotechnology Co., Ltd. (Nanjing, China). DAPI and Annexin V-FITC/PI staining kit were obtained from the Beyotime Institute of Biotechnology (Shanghai, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!