Anti mmp3 clone ep1186y

Total cell lysates were prepared in lysis buffer (150 mM NaCl, 1% NP-40, 50 mM Tris, 0.2% sodium dodecyl sulfate [SDS], 5 mM NaF) and used to detect ZFP36L1 or HSP70. All lysis buffers contained a cocktail of protease inhibitors and phosphatase inhibitors (Roche). The following antibodies were used for western blotting: rabbit polyclonal anti-ZFP36L1 (10 μg ml⁻¹, SC-134091; Santa Cruz Biotech.); rabbit polyclonal anti-HSP70 (1:1000 dilution, #4872; Cell Signaling); anti-MMP3 (clone EP1186Y, 1 μg ml⁻¹, ab52915; Abcam); anti-MMP13 (clone EP1263Y, 1:1000 dilution, ab51072; Abcam); anti-GFP (1:4000 dilution, ab290; Abcam); anti-lamin B (1:2000 dilution, SC-6216; Santa Cruz Biotech.); and anti-ERK (1:2000 dilution, 610408; BD Biosciences).

Total cell lysates were prepared in lysis buffer [150 mM NaCl, 1% NP-40, 50 mM Tris, 0.2% sodium dodecyl sulfate (SDS), 5 mM NaF] and used to detect ERRγ. Secreted proteins (MMP-3 and MMP-13) were detected after precipitation with trichloroacetic acid (TCA) from 900 μL of serum-free conditioned medium. All lysis buffers contained a cocktail of protease inhibitors and phosphatase inhibitors (Roche, Basel, Switzerland). Target bands were quantified using ImageJ densitometry software (NIH, Bethesda, MD, USA). The following antibodies were used for Western blotting: rabbit polyclonal anti-ERRγ (1:200 dilution; sc-66883) from Santa Cruz Biotechnology (Dallas, TX, USA), anti-MMP-3 (clone EP1186Y, 1 μg mL⁻¹, ab52915), and anti-MMP-13 (clone EP1263Y, 1:1000 dilution; ab51072) from Abcam Plc. (Cambridge, MA, USA).