Ab16885

Manufactured by Abcam

Sourced in United States

Ab16885 is a primary antibody that recognizes the specified target. The product details and specifications are available on the Abcam website.

Automatically generated - may contain errors

Lab products found in correlation

Trypsin edta, by Thermo Fisher Scientific (3 mentions) Ab81298, by Abcam (2 mentions) Ab32078, by Abcam (1 mentions) Ab4816, by Abcam (1 mentions) Pancreatin from porcine pancreas, by Merck Group (1 mentions) Potassium phosphate, by Merck Group (1 mentions) Misoprostol, by Merck Group (1 mentions) Collagen 1, by Merck Group (1 mentions) Pyk2 tyr 402, by Abcam (1 mentions) Src tyr 419, by Abcam (1 mentions) Hydroxyurea, by Merck Group (1 mentions) Glacial acetic acid, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Indomethacin, by Merck Group (1 mentions) Pepsin, by MP Biomedicals (1 mentions) Ab40794, by Abcam (1 mentions) Horse serum, by Thermo Fisher Scientific (1 mentions) Fibronectin 610077, by BD (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

4 protocols using ab16885

Fyn and Src Kinase Activity in Rat Striatum

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rat dorsal striatum tissue was homogenized using BioRuptor in 1× IP buffer (150 mM NaCl, 10 mM Tris-HCl pH = 7.4, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100) containing protease and phosphatase inhibitors. Homogenates were pre-cleared using protein G Dynabeads for 1 h at 4 °C. For immunoprecipitation, 1 mg of pre-cleared protein was incubated overnight at 4 °C with 7.5 µg of total Fyn (4023S, Cell Signaling, diluted 1:1000) or 5 µg of total Src (ab16885, Abcam, diluted 1:1000) antibody. Immunocomplexes were pulled down with protein G Dynabeads for 4 h at 4 °C and eluted into Laemmli buffer at 55 °C for 10 min. 50 µg of immunoprecipitated protein was used for western blotting. To determine kinase activity, antibodies against active p(Y418)-Src (ab4816, Abcam, diluted 1:200) and inactive p(Y529)-Src (ab32078, Abcam, diluted 1:1000) were used. In addition, dorsal striatal protein tyrosine kinase activity was also measured using a commercially available kit following the manufacturer’s instructions (Omnia Kinase Assay, KNZ3051, Thermo Fisher).

Egervari G., Akpoyibo D., Rahman T., Fullard J.F., Callens J.E., Landry J.A., Ly A., Zhou X., Warren N., Hauberg M.E., Hoffman G., Ellis R., Ferland J.M., Miller M.L., Keller E., Zhang B., Roussos P, & Hurd Y.L. (2020). Chromatin accessibility mapping of the striatum identifies tyrosine kinase FYN as a therapeutic target for heroin use disorder. Nature Communications, 11, 4634.

+ Open protocol

+ Expand

Protein Kinase Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dulbecco's modified Eagle's medium, Trypsin-EDTA, and Phospho-FAK (Tyr-397) recombinant rabbit monoclonal antibody (31H5L17 1:1000 dilution) were from Thermo Fisher (Waltham, MA). Antibodies to FAK-Tyr-397 (ab81298, 1:1000 dilution), Pyk2-Tyr-402 (ab4800, 1:1000 dilution), Src (ab16885, 1:1000 dilution), and Src-Tyr-419 (ab185617, 1:1000 dilution) were from Abcam (San Francisco, CA). Antibodies to total FAK (Anti-FAK, clone 4.47, 05–537, 1:1000 dilution) were from EMD Millipore, (Temecula, CA), and Pyk2 (3292 s, 1:1000 dilution) was from Cell Signaling Technology (Danvers, MA). Secondary antibodies anti-rabbit 800, anti-mouse 680, and anti-mouse 800 were from LI-COR (Lincoln, NE). Pepsin, >2000 U/mg protein was purchased from MP biomedicals (Irvine, CA). Indomethacin, misoprostol, hydroxyurea, potassium phosphate, sodium hydroxide, pancreatin from porcine pancreas, and collagen I were purchased from Sigma Aldrich (St. Louis, MO). Glacial acetic acid was purchased from Fisher Chemical (Cat# A38S-500). Mini-Ames assay and in vitro ADME (absorption, distribution, metabolism, and excretion) studies were conducted by Pharmaron Inc (Beijing, China). Mouse serum chemistries were performed by the veterinary diagnostic laboratory at Michigan State University (Lansing, MI).

Wang Q., Gallardo-Macias R., Vomhof-DeKrey E.E., Gupta R., Golovko S.A., Golovko M.Y., Oncel S., Gurvich V.J, & Basson M.D. (2022). A novel drug-like water-soluble small molecule Focal Adhesion Kinase (FAK) activator promotes intestinal mucosal healing. Current Research in Pharmacology and Drug Discovery, 4, 100147.

+ Open protocol

+ Expand

FAK Inhibitor's Impact on Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The BRACs used in this study were purchased from the New Star Natural Plant Development Company (Jilin, China). The FAK inhibitor, 1,2,4,5-benzenetetramine tetrahydrochloride (Y15), was obtained from Sigma (St. Louis, MO, USA). Fetal bovine serum (FBS) and horse serum were purchased from Gibco-BRL (Grand Island, NY, USA). Dulbecco's modified Eagle's medium (DMEM)/high glucose and DMEM/F12 were purchased from HyClone (Beijing, China). L15 medium was obtained from Keygen Biotech (Nanjing, China). Primary antibodies against FAK (ab40794), phospho-FAK (ab38458, ab81298), Src (ab16885), phospho-Src (ab24789), and p130^Cas (ab136514) were purchased from Abcam (Cambridge, MA, USA). Phospho-p130^Cas (#4011) antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). Primary antibodies against E-cadherin (610181), vimentin (550513), and fibronectin (610077) were purchased from BD Biosciences (Bedford, MA, USA).

Zhou J., Zhu Y.F., Chen X.Y., Han B., Li F., Chen J.Y., Peng X.L., Luo L.P., Chen W, & Yu X.P. (2017). Black rice-derived anthocyanins inhibit HER-2-positive breast cancer epithelial-mesenchymal transition-mediated metastasis in vitro by suppressing FAK signaling. International Journal of Molecular Medicine, 40(6), 1649-1656.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Markers

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western-blot was performed as our previous description.¹⁴ (link) Aliquots proteins were separated by SDS-PAGE (10% polyacrylamide). Thereafter, proteins were electrophoretically transferred to polyvinylidene difluoride membrane and blocked in 5% BSA and 0.05% Tween 20 in Tris-buffered saline (TBST) for 1 h at room temperature. Membranes were incubated overnight at 4°C with the indicated first antibodies. Membranes were washed (3 times for 5 min each) in TBST and incubated with horseradish peroxidase-conjugated IgG for 1 h at room temperature, followed by additional washes (3 times for 15 min) in TBST. Proteins were visualized by ECL and quantified by densitometric scanning with Image J and analyzed with GraphPad Prism software, and the result was presented by the relative intensity of control condition based on GAPDH normalization for total protein. Antibodies of CD36 (ab133625, ab23680), α7nAChR (ab216485), Src (ab16885) were purchased from Abcam (Cambridge, MA). p-Src-Tyr416 (6943S), p-Src-Tyr527 (2105S), p-Akt473 (9271s), p-Akt308 (9275S), Akt (9272S), p-ERK1/2 (4370S), ERK1/2 (4695S), GAPDH (5174S) were obtained from Cell Signaling (Boston, MA). The anti-mouse (7076) and anti-rabbit (7074) HRP-linked anti-IgGs were purchased from Cell Signaling (Boston, MA).

Li M.Y., Wang M., Dong M., Wu Z., Zhang R., Wang B., Huang Y., Zhang X., Zhou J., Yi J., Chen G.G, & Liu L.Z. (2023). Targeting CD36 determines nicotine derivative NNK-induced lung adenocarcinoma carcinogenesis. iScience, 26(8), 107477.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!