To measure co-localization of VE-cadherin in the lung, rat lungs were collected gently and then fixed in 4 % paraformaldehyde at 4 °C. Twenty four hours later, lung tissue was frozen in optimal cutting temperature medium (OCT; Sakura Finetek USA, Inc., Torrance, CA, USA) and cut into 5-μm-thick sections. Then the sections were stained with anti-VE-cadherin antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), followed by Fluorescence (FITC)-AffiniPure Donkey Anti-Rabbit IgG (H + L; Jackson ImmunoResearch Inc., PA, USA), and mounted with 4,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Fluorescence was monitored with an Olympus IX71 microscope (Olympus Co., Tokyo, Japan). The mean fluorescence intensity of VE-cadherin of five randomly chosen high-power fields per lung lobe section per rat was assessed and calculated.
Fitc affinipure donkey anti rabbit igg h l
Manufactured by Jackson ImmunoResearch
Sourced in United States, United Kingdom
The FITC)-AffiniPure Donkey Anti-Rabbit IgG (H + L) is a secondary antibody produced in donkeys and labeled with the fluorescent dye FITC (Fluorescein Isothiocyanate). It is designed to detect and bind to rabbit immunoglobulin G (IgG) molecules, including both the heavy and light chains.
Automatically generated - may contain errors
Lab products found in correlation
Ix71 microscope, by Olympus (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Anti ve cadherin antibody, by Santa Cruz Biotechnology (1 mentions)
Sarcomeric α actinin, by Merck Group (1 mentions)
Sm22 alpha, by Abcam (1 mentions)
Calponin, by Abcam (1 mentions)
Texas red affinipure donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Laminin, by Abcam (1 mentions)
Gata4, by R&D Systems (1 mentions)
Hoechst 3342, by Cell Signaling Technology (1 mentions)
3 protocols using fitc affinipure donkey anti rabbit igg h l
1
VE-cadherin Lung Co-localization Assay
2
Immunostaining of Pluripotent and Cardiac Cells
3
Immunofluorescent Staining of Cultured Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells in the logarithmic growth phase were seeded onto 24-well culture plates. After fixation with 4% paraformaldehyde for 30 min, the cells were permeabilized with 1% Triton X-100 for 20 min. Subsequently, the cells were blocked with 10% serum for 1 h, followed by overnight incubation at 4 °C with primary antibody applied dropwise. The next day, goat anti-mouse IgG H&L (Alexa Fluor® 647, ab150115, 1:100; Abcam, Cambridge, UK) and fluorescein (FITC)-AffiniPure donkey anti-rabbit IgG (H+L) (#711–095-152, 1:100; Jackson ImmunoResearch, West Grove, PA, USA) were added and incubated for 2 h at 25 °C. The nuclei were stained with Hoechst 3342 (Cell Signaling Technology), after which a fluorescent quencher was added dropwise. The final observation was performed under a fluorescence microscope with LASS2 in red, TFRC in green, and DAPI in blue.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!