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Transcription kits

Manufactured by Takara Bio
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Sourced in Japan, United States

Transcription kits are designed to facilitate the in vitro synthesis of RNA from DNA templates. The kits provide the necessary reagents, including RNA polymerase, ribonucleotides, and appropriate buffers, to enable the transcription process.

Automatically generated - may contain errors

Lab products found in correlation

Steponeplus system, by Thermo Fisher Scientific (2 mentions) Trizol, by Takara Bio (1 mentions) Trizol reagent, by Takara Bio (1 mentions)

Close spelling variants of this product

Reverse transcription and quantitative PCR (Q-PCR) kits Reverse transcription and RT-qPCR kits Genomic DNA Eraser Reverse Transcription Kits Reverse transcription and real-time quantitative polymerase chain reaction (RT-qPCR) kits Reverse Transcription Synthesis Kit Superscript II transcription kit PrimeScrip reverse transcription kit One Step SYBR PrimerScript reverse transcription (RT)-PCR kit Reverse transcription reagents kit TaqMan miRNA Reverse Transcription Kit

2 protocols using transcription kits

1

Quantitative RT-PCR Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using the Trizol (TakaRa, Otsu, Japan) method, following the manufacturer’s instructions. mRNA was reverse transcribed with transcription kits (TakaRa, Otsu, Japan) to synthesize cDNA, and the cDNA was performed using SYBR green kits on a Step one plusTM system (Thermo Fisher, Waltham, MA, USA). The primer sequences for the genes are shown in Table 1, and the data were processed using the 2−ΔΔCT method.
Wang J., Ge J., Cao H., Zhang X., Guo Y., Li X., Xia B., Yang G, & Shi X. (2019). Leptin Promotes White Adipocyte Browning by Inhibiting the Hh Signaling Pathway. Cells, 8(4), 372.
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2

RNA Extraction and qRT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using TRIzol reagent (TaKaRa, Otsu, Japan) following the manufacturer’s instructions. The mRNA was reverse transcribed with transcription kits (TaKaRa) to synthesise cDNA, and the cDNA was amplified using SYBR Green kits on a Stepone plus™ system (Thermo Fisher, Waltham, MA, USA). The primer sequences used are shown in Supplementary Table S1, and the data were processed using the 2−ΔΔCT method.
Shi X., Zhou X., Chu X., Wang J., Xie B., Ge J., Guo Y., Li X, & Yang G. (2019). Allicin Improves Metabolism in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiota. Nutrients, 11(12), 2909.
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