Homogenisation was performed according to the methodology described in the previous studies [14 (link), 16 (link)]. The samples of the tumour and NT were weighed and homogenised using a PRO200 homogeniser (PRO Scientific Inc., Oxford, CT, USA) at 10,000 rpm in 9 volumes of PBS (EURx, Gdansk, Poland). After homogenisation, sonification with an ultrasonic cell disrupter was performed (UP100H, Hielscher Ultrasonics GmbH, Teltow, BB, Germany).
Pro200 homogeniser
Manufactured by PRO Scientific
Sourced in United States, Australia
The PRO200 homogeniser is a laboratory equipment designed for the homogenisation of samples. It is a high-performance device that uses a rotary motion to effectively mix and blend various materials, ensuring a consistent and uniform end product.
Automatically generated - may contain errors
2 protocols using pro200 homogeniser
1
Tissue Homogenization for Tumor Analysis
2
Muscle Protein Extraction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Muscle extracts were prepared by homogenising muscles in ice-cold buffer [0.15% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM sodium orthovanadate (Na3VO4), 10 mM sodium fluoride (NaF), 15 mM sodium chloride (NaCl), 20 mM HEPES, 10 mM PMSF, pH 7.5] supplemented with 10 mM PMSF and 1 complete™, EDTA-free protease inhibitor cocktail tablet (Roche Diagnostics, Sydney, Australia) using a Pro200 homogeniser (PRO Scientific Inc, Sydney, Australia). Samples were incubated on ice for 1 hr and supernatant lysates were collected following centrifugation at 14,000g for 10 min at 4°C. Protein samples were divided into aliquots and kept at –80°C before subsequent experimental procedures.
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