Xevo tqd triple quadrupole mass spectrometer

Manufactured by Waters Corporation

Sourced in United States

The Xevo TQD triple quadrupole mass spectrometer is a high-performance analytical instrument designed for quantitative and qualitative analysis. It utilizes triple quadrupole technology to provide precise and sensitive detection of target analytes in complex matrices.

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Xevo TQD (77 citations) Xevo TQD mass spectrometer (37 citations) TQD mass spectrometer (32 citations) TQD TM (2 citations) XevoTM (2 citations) XEVO TQD triple quadrupoles mass spectrometer (2 citations)

43 protocols using xevo tqd triple quadrupole mass spectrometer

Quantifying Alkaloid Profiles in RC Extracts

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A UPLC/QqQ-MS system with a Waters ACQUITY UPLC H-Class connected online to a Waters Xevo Triple quadrupole (TQD) mass spectrometer (Waters, Milford, MA, USA) was applied to determine the alkaloid profiles of RC extracts according to our developed method (Zhong et al., 2020 (link)). The extracts were chromatographically separated by an ACQUITY UPLC BEH C18 (100 × 2.1 mm, 5 m) with a column temperature of 25°C and a flow rate of 0.4 mL/min. The mobile phases were 0.1% formic acid water (A) and acetonitrile (B). The following gradient program was set: 0-2 min, 85%-76% A; 2-6 min, 76%-75.5% A; 6-8 min, 75.5%-75.4% A; and 8-10 min, 75.4%-75% A. The injection volume and detection wavelength were set as 1 L and 320 nm, respectively.
The Xevo TQD mass spectrometer was conducted in positive ion mode. High purity nitrogen and helium was applied as nebulizing gas and collision gas, respectively. The mass spectrometry conditions are listed below: capillary voltage: 2.5 KV; cone voltage: 25 V; source temp: 120°C; desolvation temp: 500°C; desolvation gas: 1000 L/hr; cone gas: 50 L/hr; Full scan range: 100-1200 amu; scan mode: MSe. The RC extracts were determined based on the multiple reaction monitoring (MRM) mode and the cone voltages and collision energies were optimized according to the alkaloid reference standards.

Qi L., Zhong F., Liu N., Wang J., Nie K., Tan Y., Ma Y, & Xia L. (2022). Characterization of the anti-AChE potential and alkaloids in Rhizoma Coptidis from different Coptis species combined with spectrum-effect relationship and molecular docking. Frontiers in Plant Science, 13, 1020309.

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Monthly Urine Analysis for Biomarkers

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Participants gave morning urine samples once a month for 12 months. The first and last samples were brought to the centre, with participants posting in their urine samples during the intervening months. Urine samples were centrifuged at 2000 rpm for 10 min and stored at -20 °C until analysis of neopterin and creatinine levels. Urine neopterin and creatinine concentrations were measured via ultra-performance liquid chromatographymass spectrometry (UPLC-MS) 29 (link) . All measurements were undertaken using ACQUITY UPC interfaced with a Waters Xevo triple quadrupole (TQD) mass spectrometer equipped with an electrospray ionization probe, column oven and autosampler.

Kidd R.L., Agyemang-Prempeh A., Sanderson A., Stuart C., Mahajan S., Verschuur C.A, & Newman T.A. (2024). Longitudinal urinary neopterin is associated with hearing threshold change over time in independent older adults. Scientific reports, 14(1).

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HILIC LC-MS/MS for Ado and 2'-dAdo Metabolites

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A < 5 min LC-MS/MS method for the separation of Ado and 2′-dAdo from their metabolites, Ino and 2′-dIno, was developed on a Waters I Class Acquity UPLC system. Sample detection and quantification was performed on a Waters Xevo TQD triple quadrupole mass spectrometer in positive electrospray mode as defined above. 10 µL of sample prepared as described in section 2.4, or sample mixtures prepared from liquid calibrators, were injected onto a Supelcosil^TM LC-NH₂ 2.1 × 50 mm, 3 μm HILIC column (Sigma Aldrich, St. Louis, MO) for separation. The samples were eluted with a flow rate of 0.4 mL/min before a quick column wash at 1.2 mL/min. See Table 2 for mobile phase and gradient conditions.Table 2

Mobile phase and gradient conditions in HILIC LC-MS/MS method specific to ADA-SCID. Mobile phase (A): 97:3 acetonitrile/water, with 1% formic acid and mobile phase (B): 100% water with 1% formic acid.

Time (min)	Flow rate (mL/min)	% A	% B
0.00	0.400	100	0
1.00	0.400	100	0
2.50	0.400	93	7
4.00	0.400	88	12
4.10	0.400	100	0
5.00	1.20	100	0
5.20	0.400	100	0

Young B., Hendricks J., Foreman D., Pickens C.A., Hovell C., De Jesús V.R., Haynes C, & Petritis K. (2020). Development of dried blood spot quality control materials for adenosine deaminase severe combined immunodeficiency and an LC-MS/MS method for their characterization. Clinical Mass Spectrometry, 17, 4-11.

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Quantitative HPLC-ESI-MS/MS Analysis of 2-Oxo-Carnosine and 2-Oxo-Anserine

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Quantitative HPLC-ESI-MS/MS analysis was performed as described previously [4 (link)]. In brief, the samples (final 1 mM) were mixed with stable isotope-labeled 2-oxo-carnosine or 2-oxo-anserine as internal standards (final 500 nM), followed by the quantification of 2-oxo-carnosine and 2-oxo-anserine by HPLC-ESI-MS/MS using the Xevo TQD triple quadrupole mass spectrometer (Waters, MA, USA) coupled with the Alliance e2695 HPLC system (Waters). Samples were separated by the Alliance e2695 system with an Intrada Amino Acid column (2.0 × 50 mm; Imtakt). A discontinuous gradient of solvent A (acetonitrile containing 0.1% formic acid) and solvent B (100 mM ammonium formate) was used as follows: 0% B at 0 min, 60% B at 0.1 min, 70% B at 5 min, 99% B at 9 min, at a flow rate of 0.3 mL/min. Parameters for multiple reaction monitoring were described previously [4 (link)].

Kasamatsu S., Komae S., Matsukura K., Kakihana Y., Uchida K, & Ihara H. (2021). 2-Oxo-Imidazole-Containing Dipeptides Play a Key Role in the Antioxidant Capacity of Imidazole-Containing Dipeptides. Antioxidants, 10(9), 1434.

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UPLC-MS/MS Quantification of Poziotinib and Metabolites

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In this study, poziotinib, M1 and M2 were detected on a Waters ACQUITY UPLC I‐Class system equipped with a Waters XEVO TQD triple‐quadrupole mass spectrometer (Waters Corp.). An ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm) was selected, and the column temperature was maintained at 40°C. Mobile phase was composed of acetonitrile (solvent A) and aqueous formic acid (0.01% formic acid in water, solvent B) with 0.40 ml/min flow rate, and extracting condition was optimized as follows: 0–0.5 min, 10%–30% A; 0.5–1 min, 30%–95% A; 1–2 min, 95% A; and 2–2.3 min, 95%–10% A. Then, 10% A was maintained for 0.7 min to reach equilibrium. The injection volume was 2 μl for analysis.
Positive ion multiple reaction monitoring (MRM) mode was selected to detect the target analytes with an electrospray ionization (ESI) source. Other optimized MS parameters were listed as following: 3.0 kV of capillary voltage; 150 and 500°C of source temperature and desolvation temperature; 1000 and 50 L/h of desolvation gas flow rate and cone gas flow rate. The precursor and product ions for poziotinib, M1, M2, and IS are presented in Table 1.

Wang S., Xia M., Wang Y., Lu Z., Geng P., Dai D., Zhou Y, & Wu Q. (2023). Inhibitory effect of Schisandrin on the pharmacokinetics of poziotinib in vivo and in vitro by UPLC‐MS/MS. Thoracic Cancer, 14(14), 1276-1285.

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ESI-MS Analysis of Chemical Compounds

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ESI-MS in positive and negative modes was carried out using an Xevo TQD Triple Quadrupole Mass Spectrometer (Waters Corporation, Milford, MA, USA). LC was performed on an ACQUITY UPLC-BEH using C18 column and water/methanol gradient containing 1% formic acid. More details were described earlier [19 (link)].

Soliman G.A., Abdel-Rahman R.F., Ogaly H.A., Althurwi H.N., Abd-Elsalam R.M., Albaqami F.F, & Abdel-Kader M.S. (2020). Momordica charantia Extract Protects against Diabetes-Related Spermatogenic Dysfunction in Male Rats: Molecular and Biochemical Study. Molecules, 25(22), 5255.

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HPLC-MS/MS for Adenosine Metabolites

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A < 5 min HPLC method for the separation of Ado and 2′-dAdo from their metabolites, Ino and 2′-dIno, was developed on a Waters I Class Acquity UPLC system. Sample detection and quantification was performed on a Waters Xevo TQD triple quadrupole mass spectrometer in positive electrospray mode as defined above. 10 μL of sample prepared as described in section 2.4, or sample mixtures prepared from liquid calibrators, were injected onto a Supelcosil^™ LC-NH₂ 2.1 × 50 mm, 3 μm HILIC column (Sigma Aldrich, St. Louis, MO) for separation. The samples were eluted with a flow rate of 0.4 mL/min before a quick column wash at 1.2 mL/min. See table 2 for mobile phase and gradient conditions.

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Poziotinib Quantification by UPLC-MS/MS

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The concentrations of poziotinib were determined on a UPLC-MS/MS system, which possessed an ACQUITY I Class UPLC and a XEVO TQD triple quadrupole mass spectrometer (Waters Corp., Milford, MA, USA). The UPLC system consists of a Binary Solvent Manager (BSM) and a Sample Manager with Flow-Through Needle (SM-FTN). Chromatographic analysis of poziotinib was performed on a CORTECS C18 column (2.1 × 50 mm, 1.6 µm) maintained at 40 °C. The mobile phase consisted of 0.1% formic acid and acetonitrile, and the elution process had a linear gradient: It started with acetonitrile increasing from 10 to 30% (0–1 min); rapidly increasing from 30 to 95% (1–2 min), which was maintained at 95% (2–2.5 min); and then decreasing to 10% (2.5–2.6 min). The flow rate was 0.4 mL/min, and the total run time was 3 min. The precursor ion and product ion, which were determined by the positive MRM mode, were m/z 492.06→354.55 and m/z 474.57→456.64 for poziotinib and IS, respectively. The optimal MS parameters were defined as follows: the cone voltages were both set at 30 V for poziotinib and IS; the collision energies were set at 20 and 28 eV for poziotinib and IS, respectively.

Ji W., Shen J., Wang B., Chen F., Meng D., Wang S., Dai D., Zhou Y., Wang C, & Zhou Q. (2021). Effects of dacomitinib on the pharmacokinetics of poziotinib in vivo and in vitro. Pharmaceutical Biology, 59(1), 455-462.

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Targeted Polysulfide Metabolomics by LC-MS/MS

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LC–ESI–MS/MS analyses were performed using a Xevo TQD Triple Quadrupole Mass Spectrometer (Waters Co., Milford, MA) coupled to a Shimadzu LC-20A system (Shimadzu Co.) equipped with an autosampler (SIL-20A), a communications bus module (CBM-20A), an online degasser (DGU-20A5), two liquid chromatographs (LC-20AD), and a column oven (CTO-20A). The samples were evaluated using the Shimadzu LC-20A system on a Mightysil-C18 column (50 mm × 2.0 mm inner diameter; KANTO CHEMICAL Co.) and then eluted using methanol as the mobile phase with a linear gradient (for detection of NEM-S-NEM, 1% B at 0–1 min, 99% B at 4–5 min, 1% B at 5.1–8 min; for untargeted polysulfide omics analysis, 1% B at 0 min, 99% B at 10–12 min, 1% B at 13–18 min; for quantitative targeted polysulfide metabolomics and detection of SFN derivatives and oxidized glutathione polysulfides [GS_nG], 1% B at 0–1 min, 99% B at 7–10 min, 1% B at 10.1–15 min) in the presence of 0.1% FA at a flow rate of 0.6 mL/min at 40 °C. The mass spectrometer was operated in the positive mode with the capillary voltage and desolvation gas (nitrogen) set to 1000 V and 1000 L/h, respectively, at 500 °C. The respective adducts were detected in the multiple reaction monitoring mode using the parameters shown in Table S1.

Kasamatsu S., Owaki T., Komae S., Kinno A., Ida T., Akaike T, & Ihara H. (2023). Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts. Redox Biology, 67, 102875.

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Quantitative Analysis of Fruquintinib by UPLC-MS/MS

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The Acquity UPLC BEH C18 chromatography column (2.1 mm ×50 mm, 1.7 μm) was used to separated analytes under gradient elution using acetonitrile (mobile phase A) and 0.1% formic acid (mobile phase B) as the mobile phase. The gradient elution program was as follows: 0–0.5 mins (90% A), 0.5–1 mins (90–10% A), 1.0–2.0 mins (10% A), 2.0–2.1 mins (10–90% A), and 2.1–3.0 mins (90% A). The overall chromatographic run time was 3.0 min and the flow rate of the method was set at 0.30 mL/min. Mass spectrometric detection was performed on a XEVO TQD triple quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source (Waters Corp., Milford, MA, USA). The analytical method used positive multiple reaction monitoring mode to detect fruquintinib and diazepam (IS). The precursor-to-product ion transitions were 394.2 → 363.2 for fruquintinib and m/z 285 → 154 for IS (desolvation temperature 500°C, desolvation gas flow rate 600 L/h, the argon flow rate 150 L/h, collision 7.0 Bar). All data acquisition and instrument control were processed under the Masslynx 4.1 software (Waters Corp.).

Mei Y.B., Luo S.B., Ye L.Y., Zhang Q., Guo J., Qiu X.J, & Xie S.L. (2019). Validated UPLC-MS/MS method for quantification of fruquintinib in rat plasma and its application to pharmacokinetic study. Drug Design, Development and Therapy, 13, 2865-2871.

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