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Theta lite 101

Manufactured by Biolin Scientific
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Sourced in Sweden

The Theta Lite 101 is a contact angle measurement instrument designed to determine the wettability of surfaces. It utilizes a sessile drop method to measure the static contact angle between a liquid and a solid surface.

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Lab products found in correlation

Crossbeam 340, by Zeiss (2 mentions) 100 ftir spectrometer, by PerkinElmer (2 mentions) Libra 200 feg, by Zeiss (1 mentions)

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Theta Lite (39 citations) Theta Lite TL 101 (4 citations)

7 protocols using theta lite 101

1

Characterization of TPU and TPU/NS Membranes

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The morphologies of the pristine TPU and TPU/NS membranes were observed using scanning electron microscopy (SEM, Crossbeam 340, Zeiss, Germany), and the compositional analysis was conducted by energy-dispersive X-ray spectrometry (EDS). Based on the SEM images, the average pore size, porosity and diameters of the nano-silver particles were measured using Image J software and was read by two independent investigators. X-ray diffraction (XRD) spectra of the TPU and TPU/NS membranes were obtained with a 2θ range between 10° and 80° using an X-ray diffractometer (X’Pert Power, Panalytical B.V., Netherlands). The attenuated total reflectance Fourier transform-infrared (ATR-FTIR) spectra of the samples were obtained using an ATR-FTIR spectrometer (Nicolet 460, USA). The water contact angles of the samples were measured using a contact angle analyser (Theta Lite 101, Biolin Scientific, Sweden).
Liu M., Liu T., Chen X., Yang J., Deng J., He W., Zhang X., Lei Q., Hu X., Luo G, & Wu J. (2018). Nano-silver-incorporated biomimetic polydopamine coating on a thermoplastic polyurethane porous nanocomposite as an efficient antibacterial wound dressing. Journal of Nanobiotechnology, 16, 89.
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2

Characterization of PCL/NS Nanofibrous Mesh

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The morphology and structure of PCL/NS nanofibrous mesh were observed using scanning electron microscopy (SEM; Hitachi S-3400N; Hitachi Ltd., Tokyo, Japan), and the diameter of NS was measured using ImageJ software by two independent researchers (five SEM images). The chemical structure of the surface of PCL, PCL/DA and PCL/NS were characterized using a PerkinElmer Fourier transform infrared (FTIR) spectrometer (100S). The water contact angle was investigated by a contact angle analyzer (Theta Lite 101; Biolin Scientific, Stockholm, Sweden).
Liu M., Luo G., Wang Y., He W., Liu T., Zhou D., Hu X., Xing M, & Wu J. (2017). Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo. International Journal of Nanomedicine, 12, 6827-6840.
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3

Contact Angle Measurement Protocol

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The contact angle measurements were taken with the Biolin Scientific ThetaLite 101 tensiometer. The OneAttension software (Version 3.2 (r5971)) was utilized for collecting data from the optical tensiometer. Depending on the variability of contact angle values, 5–10 data points were collected. The average droplet volume applied to the surfaces for contact angle measurements was 10.6 µL.
Bernardy C, & Malley J. (2023). Virus Behavior after UV254 Treatment of Materials with Different Surface Properties. Microorganisms, 11(9), 2157.
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4

Wettability of Surfactant-Coated Mesh

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Water contact angle was conducted on all groups to assess the effect of mesh properties on wettability. Mesh fabricated with no surfactant, and surfactant with 0%, 2%, 4%, and 8% water internal phase were used to determine contact angle over time. Specimens were cut from the center of the mesh in approximately 1 cm × 2 cm rectangles and loaded with minimal wrinkles onto glass slides with double-sided tape. A Biolin Scientific Theta Lite 101 optical tensiometer was used to determine contact angle over a two-minute period at 30 frames per seconds. Using a glass syringe and a blunted needle, a droplet of ultra-pure water was placed on each of the dry mesh samples using a sessile drop technique. The contact angle was then analyzed using OneAttension software. For each run, time zero was determined as the time when the water droplet released from the needle tip. These samples were assessed in triplicate for a total of 9 specimens run per composition.
Johnson P.M., Knewtson K.E., Hodge J.G., Lehtinen J.M., Trofimoff A.S., Fritz D.J, & Robinson J.L. (2021). Surfactant Location and Internal Phase Volume Fraction Dictate Emulsion Electrospun Fiber Morphology and Modulate Drug Release and Cell Response. Biomaterials science, 9(4), 1397-1408.
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5

Wetting Analysis of Hydrogel Films

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Wetting of the peptide-free PMAA and IPLVVP-PMAA hydrogel film surfaces was analyzed by measuring contact angles with a ThetaLite 101 tensiometer (Biolin Scientific). A water droplet (7 μL) was placed on the hydrogel films at room temperature. An average contact angle for each film was obtained from five different spot measurements on film surfaces.
Xue B., Kozlovskaya V., Sherwani M.A., Ratnayaka S., Habib S., Anderson T., Manuvakhova M., Klampfer L., Yusuf N, & Kharlampieva E. (2018). Peptide-functionalized hydrogel cubes for active tumor cell targeting. Biomacromolecules, 19(10), 4084-4097.
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6

Surface Tension and Viscosity Measurement

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The surface tension was measured using a Theta Lite 101 optical tensiometer by the pendant drop method (Biolin Scientific). The shape of the drop suspended from a needle results from the relationship between the surface tension and gravity. The surface tension is then calculated from the shadow image of a pendant drop using OneAttension analysis software. Dynamic viscosity was determined with a Brookfield DVII + Pro viscometer at varying shear rates and at room temperature. The absorption measurements were performed using a PerkinElmer I-900 UV-vis-NIR spectrometer.
Kassem O., Pimpolari L., Dun C., Polyushkin D.K., Zarattini M., Dimaggio E., Chen L., Basso G., Parenti F., Urban J.J., Mueller T., Fiori G, & Casiraghi C. (2023). Water-based 2-dimensional anatase TiO2 inks for printed diodes and transistors. Nanoscale, 15(12), 5689-5695.
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7

Characterization of PVDF/Nanosilica Composite Films

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The morphologies of the PVDF/NS films were observed using scanning electron microscopy (SEM, Crossbeam 340, Zeiss, Germany), and the average pore size (n = 50 pores) and thickness of each film were measured using Image J software. The morphology of the synthetic NS was observed using transmission electron microscopy (TEM, Zeiss LIBRA 200 FEG, Germany) by dropping 5 μL of mixture solution onto a carbon-coated copper grid. The Fourier transform infrared (FTIR) spectra of PVDF, PVDF/NS10, PVDF/NS25 and PVDF/NS50 films were acquired using a PerkinElmer FTIR spectrometer (100S). The water contact angles were detected using a contact angle analyzer (Theta Lite 101, Biolin Scientific, Sweden).
Liu M., Wang Y., Hu X., He W., Gong Y., Hu X., Liu M., Luo G., Xing M, & Wu J. (2018). Janus N,N-dimethylformamide as a solvent for a gradient porous wound dressing of poly(vinylidene fluoride) and as a reducer for in situ nano-silver production: anti-permeation, antibacterial and antifouling activities against multi-drug-resistant bacteria both in vitro and in vivo. RSC Advances, 8(47), 26626-26639.
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