miR148b-5p mimics, inhibitor, negative mimic control, and negative inhibitor control were purchased from Gene Pharma. Negative mimic control and negative inhibitor control were mixed to be identified as the control group. Mimics or negative control at a final concentration of 50 nM and inhibitor at a final concentration of 100 nM were mixed with Lipofectamine 2000 (Gene Pharma) according to the manufacturer's instructions and transfected into hucMSCs cultured to 70%–80% confluence in a culture dish.
Negative inhibitor control
Manufactured by GenePharma
The Negative Inhibitor Control is a laboratory reagent used to establish a baseline for biological assays. It serves as a reference point to measure the effects of potential inhibitors or modulators on a target system. The Negative Inhibitor Control does not exhibit any inhibitory activity, allowing researchers to differentiate the specific effects of the test compounds from background interference.
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Lab products found in correlation
2 protocols using negative inhibitor control
1
miR-148b-5p Mimics and Inhibitors in hucMSCs
2
Regulation of VEGFA by miR-140-5p in CRC
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The miR-140-5p mimic, normal mimic control, miR-140-5p inhibitor and negative inhibitor control were chemically synthesized by Shanghai GenePharma Company (Shanghai, China). VEGFA siRNA and its scramble siRNA control were also purchased from GenePharma Company. The sequence for VEGFA siRNA and scramble siRNA were as follows: VEGFA siRNA, sense: 5'-GGCAGAAUCAUCACGAAGUTT-3', antisense: 5'-ACUUCGUGAUGAUUCUGCCTT-3'; scramble siRNA, sense: 5'-UUCUCCGAACGUGUCACGUTT-3', antisense: 5'-ACGUGACACGUUCGGAGAATT-3'. CRC Cells were plated in 6-well plates and transfected with 100nM of the mimic, mimic control, inhibitor, inhibitor control using Lipofectamine 2000 (Invitrogen) according to the manufacture's protocol. VEGFA siRNA or scramble siRNA were transfected using the same approach.
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