Hscrp

Manufactured by R&D Systems

Sourced in United States

HsCRP is a laboratory instrument designed for the quantitative determination of high-sensitivity C-reactive protein (hs-CRP) in human serum or plasma samples. It is a sensitive assay that can detect low levels of CRP, which may be associated with inflammation and cardiovascular risk.

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Lab products found in correlation

Interleukin 6 (il 6), by R&D Systems (6 mentions) Zonulin, by PromoCell (2 mentions) Scd163, by R&D Systems (2 mentions) Scd14, by R&D Systems (2 mentions) I fabp, by R&D Systems (2 mentions) D dimer, by Diagnostica Stago (2 mentions) Insulin, by Mercodia (2 mentions) Tnf α, by R&D Systems (2 mentions) High sensitivity elisa, by Thermo Fisher Scientific (1 mentions) Vitro 950 chemistry station, by Ortho Clinical Diagnostics (1 mentions) Ox ldl, by R&D Systems (1 mentions) Oxldl, by Mercodia (1 mentions) Stnfri, by R&D Systems (1 mentions) Lipopolysaccharide binding protein, by Hycult Biotech (1 mentions) Plasma insulin, by Merck Group (1 mentions) Oxidized ldl, by Mercodia (1 mentions) Stnf rii, by R&D Systems (1 mentions) Enzyme linked immunosorbent assay, by R&D Systems (1 mentions) Adiponectin, by R&D Systems (1 mentions) Cholesterol, by Merck Group (1 mentions)

15 protocols using hscrp

Plasma Cytokine and NT ProBNP Profiling

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As outlined above, all participants underwent analysis of plasma markers of Tumor Necrosis Factor alpha (TNF-α), Interleukin-6 (IL-6), high sensitivity C-Reactive Protein (CRP), IL-8, and IL-10 at five separate time periods. N-terminal prohormone of brain natriuretic peptide (NT ProBNP) was also assessed. All blood samples were drawn into blood collection tubes from the antecubital vein (with the participant in a seated position) and then immediately immersed in a refrigerated centrifuge and centrifuged within 15 min of collection. Plasma levels of each cytokine (TNF-α, IL-6, IL-8, IL-10, and CRP) and NT ProBNP were measured using a commercially available high-sensitivity ELISA (enzyme-linked immunosorbent assay) kits [Biosource (IL-8, IL-10, TNF-α), Biocheck (hsCRP), R & D Systems (IL-6)] according to the manufacturer's instructions.

Taylor A.G., Ignaszewski A.I., Bredin S.S., Hill J.S., Shellington E.M, & Warburton D.E. (2022). High Intensity Interval Training Leads to Similar Inflammatory Activation as Seen With Traditional Training in Chronic Heart Failure. Frontiers in Cardiovascular Medicine, 8, 752531.

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Cardiovascular Disease Biomarker Analysis

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Whole blood was collected for laboratory analysis of inflammatory and lipid markers. Tumor necrosis factor (TNF-α), high sensitivity c-reactive protein (hs-CRP), oxidized low-density lipoprotein (ox-LDL), Low-density lipoproteins (LDL), apolipoprotein-B (apo-B), and triglycerides were selected based on their biological roles in CVD.
TNF-α (R&D Systems, Minneapolis, MN), hs-CRP (BN-100 nephelmometer, Dade Behring, Deerfield, IN), and ox-LDL (Mercodia AB, Uppsala, Sweden) were assessed in blood plasma by ELISA following their respective manufacturers’ directions. Triglycerides, total cholesterol, and high-density lipoproteins (HDL) were measured on the Vitro 950 Chemistry Station (Ortho-Clinical Diagnostics, Raritan, NJ) as per the manufacturer’s directions. LDL was calculated based on the subtraction of HDL from the total cholesterol measurements. Apo-B was assessed on a protein analyzer as per the manufacturer (BN ProSpec System, Dade Behring).

Liberda E.N., Zuk A.M, & Tsuji L.J. (2019). Complex contaminant mixtures and their associations with intima-media thickness. BMC Cardiovascular Disorders, 19, 289.

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Biomarker Quantification in Gut Inflammation

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Zonulin (Promocell Germany), BDG (Mybiosource Inc. CA), I-FABP, sCD14, sCD163, sTNFRI, hsCRP, IL-6 (R &D Systems, Minneapolis, Minnesota, USA) and oxidized LDL (ALPCO, Salem, New Hampshire, USA and Mercodia, Uppsala, Sweden) were measured by ELISA . The intra-assay variability ranged between 4-8% and inter-assay variability was less than 10% for all markers. All assays were done at Dr Funderburg’s laboratory at Ohio State University, Columbus, OH (https://u.osu.edu/caffre/people/nicholas-t-funderburg-ph-d/). Laboratory personnel were blinded to group assignments.

TOE S., NAGY M., ALBAR Z., Yu J., SATTAR A., NAZZINDA R., MUSIIME V., ETAJAK S., WALYAWULA F., MCCOMSEY G.A., ATUYAMBE L.M, & DIRAJLAL-FARGO S. (2022). Ambient Air Pollution is Associated with Vascular Disease in Ugandan HIV positive Adolescents. AIDS (London, England), 36(6), 863-870.

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Inflammatory Biomarker Measurement Protocol

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The blood collected was stored at −80°C and batched until processing without a prior thaw. Then using enzyme-linked immunosorbent assay (ELISA), we measured the following inflammatory markers: soluble tumor necrosis factor receptors I (sTNFR-I), High sensitivity C reactive protein (hs-CRP), interleukin 6 (IL-6) (R&D systems, Minneapolis, Minnesota, USA), and D-dimer (Diagnostica Stago, USA). We also measured by ELISA oxidized low-density lipoprotein (Ox-LDL) (Uppsala, Mercodia, Sweden) and two gut markers; lipopolysaccharide-binding protein (LBP) (Hycult Biotech Inc. Pennsylvania, USA), a marker of microbial translocation, and zonulin (Promocell, Germany), a marker of gut permeability. All measurements were done at Dr. Funderburg’s laboratory at Ohio State University, Columbus, Ohio.

Mouchati C., Durieux J.C., Zisis S.N., Labbato D., Rodgers M.A., Ailstock K., Reinert B.L., Funderburg N.T, & McComsey G.A. (2023). Increase in gut permeability and oxidized ldl is associated with post-acute sequelae of SARS-CoV-2. Frontiers in Immunology, 14, 1182544.

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Plasma Glucose and Inflammation Biomarkers

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Plasma glucose was analyzed by a glucose oxidase assay (YSI Instruments 2700, Yellow Springs, OH). Remaining samples were centrifuged at 4°C for 10 min at 3000 RPM and stored at -80°C until analysis. Plasma insulin (Millipore, Billerica, MA) as well as hs-CRP, VCAM, and ICAM (R&D Systems, Minneapolis, MN) were batched analyzed in duplicate to minimize variance within conditions using ELISA.

Malin S.K., Gilbertson N.M., Eichner N.Z., Heiston E., Miller S, & Weltman A. (2019). Impact of Short-Term Continuous and Interval Exercise Training on Endothelial Function and Glucose Metabolism in Prediabetes. Journal of Diabetes Research, 2019, 4912174.

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Biomarker Quantification in Metabolic Study

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Plasma levels of glucose (BioAssay Systems, Hayward, CA) and insulin (Mercodia, Uppsala, Sweden), as well as leptin, adiponectin, high sensitivity C-reactive protein (hsCRP), and interleukin-6 (IL-6; R&D Systems, Minneapolis, MN) were measured by enzyme-linked immunosorbent assay (ELISA). Serum levels of total, HDL, and LDL cholesterol and triglycerides were determined in the clinical chemistry laboratory at MSKCC. Coefficients of variation for intra-assay variation for quality control samples were less than 7%.

Iyengar N.M., Zhou X.K., Gucalp A., Morris P.G., Howe L.R., Giri D.D., Morrow M., Wang H., Pollak M., Jones L.W., Hudis C.A, & Dannenberg A.J. (2015). Systemic Correlates of White Adipose Tissue Inflammation in Early-Stage Breast Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(9), 2283-2289.

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Biomarkers of Immune Activation and CVD Risk in HIV

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Measurements were performed by the Dahms Clinical Research Unit Core Laboratory [Dr McComsey, laboratory Director]. In this analysis, we selected biomarkers based on prior data in HIV suggesting their association with CVD, metabolic complications, or overall mortality. Intestinal fatty acid binding protein (I-FABP) is considered a marker of enterocyte damage and has been associated with increased mortality[20 (link), 21 (link)]. Soluble CD14 (sCD14) is a soluble marker of monocyte activation and microbial translocation and is associated with mortality and progression of atherosclerosis[26 ]. Oxidized lipids (oxidized LDL) is the form of LDL that has undergone oxidative changes and has been shown to contribute to endothelial cell activation[22 (link)]. The remaining biomarkers [soluble CD163 (sCD163), interleukin-6 (IL-6), and soluble tumor necrosis alpha receptor II (sTNFRII)] correlate with cardiometabolic complications, or drive other hallmarks of immune dysregulation in HIV.
I-FABP, sCD14, sCD163, sTNFRII, hsCRP, IL-6 (R &D Systems, Minneapolis, Minnesota, USA) and oxidized LDL (Mercodia, Winston Salem, North Carolina, USA) were measured by ELISA. The intra-assay variability ranged between 4–8% and inter-assay variability was less than 11% for all markers. Laboratory personnel were blinded to group assignments.

DIRAJLAL-FARGO S., YU J., ALBAR Z., SATTAR A., MAHTAB S., JAO J., MYER L., ZAR H.J, & MCCOMSEY G.A. (2020). MONOCYTE ACTIVATION AND GUT BARRIER DYSFUNCTION IN SOUTH AFRICAN YOUTH ON ART AND THEIR ASSOCIATIONS WITH ENDOTHELIAL DYSFUNCTION. AIDS (London, England), 34(11), 1615-1623.

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Biomarkers of Inflammation, Coagulation, and Gut Barrier

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Blood was stored at −80°C and batched until processing without prior thaw. Enzyme-linked immunosorbent assay assays were performed to measure markers of systemic inflammation (soluble tumor necrosis factor receptors I and II, high sensitivity C-reactive protein [hsCRP], interleukin 6 [IL-6] [R &D Systems, Minneapolis, Minnesota]), coagulation:d-dimer (Diagnostica Stago, Parsippany, New Jersey), and oxidized low-density lipoprotein assays (Uppsala, Mercodia, Sweden), as well as markers of monocyte activation soluble CD14 and CD163 (R&D Systems, Minneapolis, Minnesota).^{[20 (link)–23 (link)]} The soluble intercellular adhesion molecule 1 was measured by Enzyme-linked immunosorbent assays (R&D Systems, Minneapolis, Minnesota).^{[24 (link)]} A marker of gut permeability: zonulin-1 (Promocell Germany), a marker of microbial translocation (Levels of lipopolysaccharide-binding protein [LBP, Hycult Biotech Inc. Pennsylvenia]), and a marker of fungal translocation β-D-glucan (Mybiosource Inc. California) were measured to assess gut-barrier integrity.^{[25 (link)–27 (link)]}

Mouchati C., El Kamari V., Sattar A., Yu J, & McComsey G.A. (2022). Comprehensive assessment of neurocognitive function, inflammation markers, and adiposity in treated HIV and control. Medicine, 101(42), e31125.

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Cytokine and Chemokine Profiling

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Cytokine and chemokine concentrations were determined in supernatants using DuoSet ELISA for TNFα, IL-6, IL-8, IL-10, IL-1β, IL-17 and IL-22 (R&D), and interferon gamma (IFN-γ) (Sanquin). Plasma hsCRP concentrations were obtained (R&D). Circulating IL-1β, IL-1Ra, IL-6 and IL-18 concentrations were sensitively measured using SimplePlex cartridge on the Ella platform (Protein Simple, San Jose, USA).

Noz M.P., Plachokova A.S., Smeets E.M., Aarntzen E.H., Bekkering S., Vart P., Joosten L.A., Netea M.G, & Riksen N.P. (2021). An Explorative Study on Monocyte Reprogramming in the Context of Periodontitis In Vitro and In Vivo. Frontiers in Immunology, 12, 695227.

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Quantification of Metabolic Biomarkers

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ELISA and FFA assay kits were performed using commercially available mouse ELISA kits. ELISA kits for insulin (Novusbio), hs-CRP (R&D systems), and VLDL (MyBioSource, San Diego, CA, USA) were used according to the manufacturer’s instructions. The assay sensitivity was < 0.19 ng/mL for insulin, < 0.015 ng/mL for hs-CRP, and < 0.195 ng/mL for VLDL. The intra- and inter-assay coefficients of variance were < 5.9% and < 6.4% for insulin, < 7.7% and < 10.8% for hs-CRP, and < 8% and < 10% for VLDL, respectively. Quantification of FFA was performed using commercially assay kit (Abcam), according to the manufacturer’s instructions.

Kim J., Lee S.K., Jeong S.Y., Cho H.J., Park J., Kim T.M, & Kim S. (2021). Cargo proteins in extracellular vesicles: potential for novel therapeutics in non-alcoholic steatohepatitis. Journal of Nanobiotechnology, 19, 372.

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