A001 3

Manufactured by Nanjing Jiancheng

Sourced in China

The A001-3 is a laboratory equipment product. It is designed to perform specific functions within a laboratory setting. The core function of the A001-3 is to enable controlled and precise measurements or operations required in various scientific experiments and research applications.

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Lab products found in correlation

Close spelling variants of this product

A001-3-2 (56 citations) SOD (A001-3-2) (12 citations) A001-3-1 (5 citations) SOD assay kit (A001-3 (5 citations) SOD assay kit (A001-3-2; (3 citations) GSH (A001-3-2) (2 citations) Superoxide dismutase (SOD) assay kit (A001-3-2) (2 citations) SOD detection kit A001-3 (2 citations)

50 protocols using a001 3

Measuring Hippocampal SOD Activity

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The activity of SOD was analyzed using kits according to the protocols from manufacturers (A001-3, Nanjing Jiancheng Biotechnology Institute, Jiangsu, China). Activation of SOD in mice’s hippocampus was detected.

Xu X., Zhou B., Liu J., Ma Q., Zhang T, & Wu X. (2023). Ru360 Alleviates Postoperative Cognitive Dysfunction in Aged Mice by Inhibiting MCU-Mediated Mitochondrial Dysfunction. Neuropsychiatric Disease and Treatment, 19, 1531-1542.

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ROS-scavenging Activities of Cell Lines

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Equal amounts of each of experimental cell lines suspended in a complete medium were seeded in 6-well plates (4 × 10⁵ cells/well) and then allowed for growth until being completely adherent. Thereafter, they were treated for different time periods (i.e., 0, 4, 16 h) with 50 μM of tBHQ. All groups of experimental cells had been collected and subjected to assays for ROS-scavenging activities of superoxide dismutase (SOD), that were determined according to the instruction of enhanced SOD assay kit (A001-3, Nanjing Jiancheng, Nanjing, China). Besides, another ROS-scavenging enzyme catalase (CAT) activity was detected through the instructions of CAT kit (BC0205, Solarbio, Beijing, China).

Wufuer R., Fan Z., Liu K, & Zhang Y. (2021). Differential Yet Integral Contributions of Nrf1 and Nrf2 in the Human HepG2 Cells on Antioxidant Cytoprotective Response against Tert-Butylhydroquinone as a Pro-Oxidative Stressor. Antioxidants, 10(10), 1610.

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Fly Metabolism and Antioxidant Assays

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The flies were crossed on standard food or RJ food. A few days later, hatched adult flies from standard food or RJ food were collected and cultured on standard food or RJ food. The 7-day-old male flies were homogenized in a buffer solution of phosphate-buffered saline (PBS) and then centrifuged for 10 min at 13,000 g. The supernatant was transferred to a new tube, the MDA (A003-1, Jiancheng Bio-Engineering, Nanjing, China), SOD oxidase (A001-3, Jiancheng Bio-Engineering, Nanjing, China), CAT oxidase (A007-1, Jiancheng Bio-Engineering, Nanjing, China), triglyceride (F001-1, Jiancheng Bio-Engineering, Nanjing, China), and ATP (A095-1-1, Jiancheng Bio-Engineering, Nanjing, China) kits were used to measure the amounts of components, according to the manufacturer's instructions. The total protein was measured using the BCA Assay Kit (CW0014, CoWin Biosciences, Beijing, China) and used for normalization. The resulting data were analyzed using one-way ANOVA.

Cheng Y., Cai J., Fu Y., Feng C., Hao Y, & Wei Y. (2020). Royal jelly attenuates metabolic defects in a Drosophila mutant with elevated TORC1 activity. Biology Open, 9(11), bio054999.

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Quantification of MDA and SOD Activities

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The activities of MDA and SOD were performed by using the commercial kits (A003-1 and A001-3, Jiancheng Bioengineering, China). Briefly, cells were homogenized by an ultrasonic equipment and the protein concentration was determined using a micro-spectrophotometer. Then, the detailed procedure for hUC-MSCs or serum samples were followed the manufacturer's protocol. Finally, the absorbance from different samples were measured by a full-wavelength microplate reader.

Ma S., Zhou X., Wang Y., Li Z., Wang Y., Shi J, & Guan F. (2022). MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway. Redox Biology, 53, 102325.

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Quantifying Superoxide Dismutase Activity

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Superoxide dismutase (SOD) activities were assayed using ELISA kits (A001-3; Nanjing Jiancheng Biology Engineering Institute, Nanjing, China), and absorbance was measured using an ultraviolet spectrophotometer at 450 nm.

Zhou J., Chen H., Du J., Tai H., Han X., Huang N., Wang X., Gong H., Yang M, & Xiao H. (2022). Glutamine Availability Regulates the Development of Aging Mediated by mTOR Signaling and Autophagy. Frontiers in Pharmacology, 13, 924081.

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Measuring Serum Stress and Antioxidant Indexes

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Serum HS indexes (HSP70, HSP90) were determined using commercially available broiler-specific kits (ELISA kits, Shanghai Enzyme-linked Biotechnology Co., Ltd., China), and serum antioxidant indexes (SOD, T-AOC) were measured using assay kits (A001-3, A015-2, Nanjing Jiancheng Biological Engineering Research Institute, China).

Yang B., Gao Y., Xi K., Wang H., Yan M., Sun H., Lin Y., Zheng X., Li Y., Guo S, & Liu C. (2024). Effects of Ban Lian Zi Jin San on intestinal inflammation and barrier function of heat-stressed broilers. Poultry Science, 103(3), 103425.

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SOD and MDA Quantification Protocol

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Under the guidance of the instructions in SOD assay (A001-3, Nanjing Jiancheng Bioengineering Institute, Jiangsu, China) and MDA assay (A003-1, Nanjing Jiancheng Bioengineering Institute, Jiangsu, China), the working liquid was prepared and added to each group of the preserved cell supernatant. After the reaction, the absorbance value was detected with a microplate reader. The content of SOD and MDA was calculated by the formula in the instructions.

Liang Y., Zhang Q., Zhang L., Wang R., Xu X, & Hu X. (2019). Astragalus Membranaceus Treatment Protects Raw264.7 Cells from Influenza Virus by Regulating G1 Phase and the TLR3-Mediated Signaling Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2019, 2971604.

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Antioxidant Enzyme Evaluation in Liver

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Liver samples were homogenized and then centrifuged to obtain the supernatants for detection of lipid peroxidation and activities of antioxidant enzymes. The enzyme activity of hepatic superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and hepatic malondialdehyde (MDA) levels were determined with kits (A001-3, A007-1, A005 and A003-1, Jiancheng, Nanjing, China) following the manufacturer’s instructions. The values were standardized to the total protein content.

Jiang S., Guo T., Guo S., Gao J., Ni Y., Ma W, & Zhao R. (2021). Chronic Variable Stress Induces Hepatic Fe(II) Deposition by Up-Regulating ZIP14 Expression via miR-181 Family Pathway in Rats. Biology, 10(7), 653.

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Antioxidant Activity of MSC-EVs in Hippocampal Neurons

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The antioxidant activity of MSC-EVs on H₂O₂-stimulated primary cultures of hippocampal neurons was tested according to the procedures described in our recent study 21 (link). The cell supernatant in each group (n = 6~9 samples per group) was collected by centrifugation at 12000 g for 5 min, and the samples and reaction reagents, including those from the ferric ion reducing antioxidant power (FRAP) (A015-3, Nanjing Jiancheng Bioengineering Institute, China), catalase (CAT) (S0051, Beyotime, China), glutathione peroxidase (GSH-PX) (A005, Nanjing Jiancheng Bioengineering Institute, China) and superoxide dismutase (SOD) (A001-3, Nanjing Jiancheng Bioengineering Institute, China) kits, were added to 96-well flat-bottom plates. The antioxidant effect or enzyme activity was detected using a microplate reader (Bio-Rad Laboratories Inc., Hercules, CA, USA) per the manufacturer's instructions. Each test was replicated 3 times.

Luo Q., Xian P., Wang T., Wu S., Sun T., Wang W., Wang B., Yang H., Yang Y., Wang H., Liu W, & Long Q. (2021). Antioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damage. Theranostics, 11(12), 5986-6005.

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Assessing Oxidative Stress in Frontal Lobe

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The SOD activity in the area of frontal lobe, which was adjacent to blood injection site, was measured according to xanthine oxidase method via a standard assay kit (Nanjing Jiancheng Bioengineering Institute, #A001‐3, PubMed: 24505260; Nanjing, China). The xanthine‐xanthine oxidase system was used to produce superoxide ions, which reacted with 2‐(4‐iodophenyl)‐3‐(4‐nitrophenol‐5‐phenlyltetrazolium chloride). The rate of the reduction with O2 was linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD; therefore, the value of SOD activity in frontal lobe could be defined via spectrophotometric measurement.
Lipid peroxidation was evaluated by measuring MDA concentrations according to the thiobarbituric acid (TBA) method through a standard assay kit (Nanjing Jiancheng Bioengineering Institute, #A003‐1, PubMed: 24058471). The principle of this assay was based on the reaction of TBA and MDA, which could be determined via spectrophotometric measurement.
These abovementioned procedures were performed according to the manufacturer's instructions. The values of SOD and MDA in all groups were averaged from several brain samples (n = 6).

Cai J., Xu D., Bai X., Pan R., Wang B., Sun S., Chen R., Sun J, & Huang Y. (2017). Curcumin mitigates cerebral vasospasm and early brain injury following subarachnoid hemorrhage via inhibiting cerebral inflammation. Brain and Behavior, 7(9), e00790.

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