Bms213hs

Manufactured by Thermo Fisher Scientific

Sourced in United States

The BMS213HS is a high-speed centrifuge designed for laboratory use. It features a maximum speed of 21,000 rpm and a maximum relative centrifugal force of 53,600 x g. The centrifuge is capable of accommodating a variety of rotor types and sample volumes.

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Lab products found in correlation

Bms223hs, by Thermo Fisher Scientific (5 mentions) Amicon ultra 15 10k, by Merck Group (1 mentions) Adi 917 020, by Enzo Life Sciences (1 mentions) Rag018r, by BioVendor (1 mentions) Ehpge2, by Thermo Fisher Scientific (1 mentions) Khc3011, by Thermo Fisher Scientific (1 mentions) Synergy 2 elisa plate reader, by Agilent Technologies (1 mentions) Hk315 01, by Hycult Biotech (1 mentions) Ready set go elisa kit, by Thermo Fisher Scientific (1 mentions) Biotek synergy ht, by Agilent Technologies (1 mentions) Synergy ht, by Agilent Technologies (1 mentions) Bms2038inst, by Thermo Fisher Scientific (1 mentions) Mak041, by Merck Group (1 mentions) Ab179886, by Abcam (1 mentions)

16 protocols using bms213hs

Rheumatoid Arthritis Disease Activity Profiles

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The clinical data included age, sex, disease duration, 66/68-joint count, patient’s pain and global assessment (0~100 scale), physician’s global assessment (0~100 scale), Health Assessment Questionnaire Disability Index (HAQ-DI, 0~3), DAS28, SDAI and Clinical Disease Activity Index (CDAI). DAS28 scores were calculated by using the 66/68 joint count. Therefore, an involvement of over 28 joints was considered as the involvement of 28 joints. Patients were divided into two groups by disease activity at 24 weeks: high disease activity group (HDA at 24 weeks; DAS28>5.1) or remission, low and moderate disease activity group (non HDA at 24 weeks; DAS28≤5.1).
Laboratory data included rheumatoid factor (U/mL), ESR (mm/h), CRP (mg/dL), IL-6 (pg/mL), and soluble IL-6 receptor (sIL-6R) (ng/mL). The levels of IL‐6 were measured by high‐sensitivity Enzyme-Linked Immunosorbent Assay (ELISA) (BMS213HS; Bender MedSystems, Vienna, Austria) and sIL-6R by ELISA (BMS214; Bender MedSystems) according to the manufacturer’s instructions. Serum was collected every 4 weeks.

Yeo J., Baek H.J., Song Y.W, & Lee E.Y. (2022). Evaluation of Serum Matrix Metalloproteinase-3 as an Objective Indicator for the Disease Activity in Rheumatoid Arthritis Patients Treated With Methotrexate Versus Tocilizumab: 24-week Results From a Prospective Randomized Controlled Study. Journal of Rheumatic Diseases, 29(2), 89-97.

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Biomarker Quantification in Sleep Study

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Blood samples were taken at the end of the ten-minute supine rest after the sleep study. Samples were subjected to a routine laboratory procedure to have the serum separated. ET-1, CRP, IL-6, TNF-α, and VEGF were measured with Enzyme-Linked Immunosorbent Assay kits (ET-1, ADI-900-202A, Enzo Life Sciences Inc., New York, USA; CRP, 88-7502, eBioscience, Inc., California, USA; IL-6, BMS213HS, Bender MedSystems GmbH, Vienna, Austria; TNF-α, BMS223HS, Bender MedSystems GmbH, Vienna, Austria; VEGF, BMS277/2, Bender MedSystems GmbH, Vienna, Austria). The metabolites of NO were measured by the Griess reaction using prepared kits (ADI-917-020, Enzo Life Sciences Inc., New York, USA) before which samples were ultrafiltered through a 10,000 MWCO filter (Amicon® Ultra-15 10K, Merck Millipore Ltd., County Cork, Ireland) to remove protein as recommended.

Wu Y., Huang R., Zhong X, & Xiao Y. (2017). Cardiovascular Consequences of Repetitive Arousals over the Entire Sleep Duration. BioMed Research International, 2017, 4213861.

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Biomarker Profiling of Inflammatory and Metabolic Factors

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The plasma concentration of TNFα, interleukin (IL)-6, IL-10, leptin, adiponectin, angiotensinogen, and irisin was measured using an enzyme-linked immunosorbent assay kit (eBioscience BMS223HS, BMS213HS, BMS215HS, Thermo Fisher Scientific, Waltham, MA, USA; Biovendor RD191001100, Brno, Czech Republic; Assaypro EA3501-1, St Charles, MO, USA; IBL International 27412, Hamburg, Germany; Biovendor RAG018R, Brno, Czech Republic). To determine malondialdehyde (MDA) levels, plasma samples were measured using the thiobarbituric acid-reactive substance (TBARS) method [17 (link)]. Briefly, we mixed samples with 20% acetic acid (pH 3.5), 0.8% thiobarbituric acid, and 8.1% sodium dodecyl sulfate and incubated the mixture at 95 °C for 1 h. After the mixture had cooled down to room temperature, trichloroacetic acid was added and detected spectrophotometrically at 532 nm.

Cheng W.Y., Yeh W.J., Ko J., Huang Y.L, & Yang H.Y. (2022). Consumption of Dehulled Adlay Improved Lipid Metabolism and Inflammation in Overweight and Obese Individuals after a 6-Week Single-Arm Pilot Study. Nutrients, 14(11), 2250.

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Quantification of Serum IL-6 Levels

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IL-6 was measured in serum samples of patients using a high-sensitivity IL-6 ELISA kit according to the manufacturer’s instructions (Thermo Fisher Scientific Cat# BMS213HS, RRID: AB_2575453, Thermo Fisher, Waltham, USA). Lower limit of detection (LLOD) according to the manufacturer 0.08 pg/ml.

Huehnchen P., Muenzfeld H., Boehmerle W, & Endres M. (2020). Blockade of IL-6 signaling prevents paclitaxel-induced neuropathy in C57Bl/6 mice. Cell Death & Disease, 11(1), 45.

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Quantifying IL-6 and IL-8 Levels

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Medium from cells treated with or without doxorubicin were collected. 50 μL samples were analyzed in IL-6 and IL-8 ELISA plates (Thermo Scientific, Cat # BMS213HS and KHC0081) and quantified according to standard curves.

Hu X, & Zhang H. (2019). Doxorubicin-Induced Cancer Cell Senescence Shows a Time Delay Effect and Is Inhibited by Epithelial-Mesenchymal Transition (EMT). Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 3617-3623.

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Quantification of Secreted Inflammatory Factors

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The quantification of the secreted levels of IL-1α (BMS243-2 Thermo Fisher Scientific, Waltham, MA, USA), PGE2 (EHPGE2, Thermo Fisher Scientific), IL-6 (BMS213HS, Thermo Fisher Scientific), MMP-1 (RAB0361, Sigma Aldrich), and TIMP-1(RAB0466, Sigma Aldrich) was performed using commercially available ELISA kits according to the manufacturer’s instructions.

Ferrà-Cañellas M.D., Munar-Bestard M., Floris I., Ramis J.M., Monjo M, & Garcia-Sureda L. (2023). A Sequential Micro-Immunotherapy Medicine Increases Collagen Deposition in Human Gingival Fibroblasts and in an Engineered 3D Gingival Model under Inflammatory Conditions. International Journal of Molecular Sciences, 24(13), 10484.

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Quantifying Serum Inflammatory Markers

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IL6 and TNFα concentrations were measured in frozen serum using enzyme-linked immunosorbent assay (ELISA) systems. We used an aliquot for this purpose. In particular, TNFα and IL6 protein levels were estimated with a high sensitivity ELISA kit (#BMS223HS and #BMS213HS, Human IL-6 High Sensitivity ELISA, Thermo Fisher Scientific, Waltham, MA, United States), according to the manufacturers’ instructions. Serum TNFα and IL6 levels were expressed as pg/mL.

Olivares-González L., Salom D., González-García E., Hervás D., Mejía-Chiqui N., Melero M., Velasco S., Muresan B.T., Campillo I., Vila-Clérigues N., López-Briz E., Merino-Torres J.F., Millán J.M., Soriano Del Castillo J.M, & Rodrigo R. (2022). NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial. Frontiers in Nutrition, 9, 847910.

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Quantifying Inflammatory Cytokines in CM

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The collected conditioned media (CM) samples (Microglia preparation section) were tested for TNFα (Thermo Fisher Scientific, KHC3011), IL1β, IL 6 (Thermo Fisher Scientific, BMS213HS) and IFNγ were assessed using their respective ELISA kits, following the manufacturer's instructions. A Synergy 2 ELISA plate reader (BioTek, Winooski, VT, USA) was used to quantify ELISA signals. according to the manufacturer's instructions.

Ambrin G., Kang Y.J., Van Do K., Lee C., Singh B.R, & Cho H. (2024). Botulinum Neurotoxin Induces Neurotoxic Microglia Mediated by Exogenous Inflammatory Responses. Advanced Science, 11(15), 2305326.

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Inflammatory Biomarkers in Response to Study Bread

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Inflammatory biomarkers, interleukin 8 (IL-8), interleukin 6 (IL-6) and lipopolysaccharide binding protein (LBP), were measured at baseline and after the treatment period with study breads. Serum IL-6 (BMS213HS, ThermoFisher Scientific, Waltham, MA USA), IL-8 (HS800, R&D Systems, Abingdon, UK) and LBP (HK315-01, LBP; Hycult Biotech, Uden, The Netherlands) levels were quantified using sensitive enzyme-linked immunosorbent assays (ELISA).

Laatikainen R., Koskenpato J., Hongisto S.M., Loponen J., Poussa T., Huang X., Sontag-Strohm T., Salmenkari H, & Korpela R. (2017). Pilot Study: Comparison of Sourdough Wheat Bread and Yeast-Fermented Wheat Bread in Individuals with Wheat Sensitivity and Irritable Bowel Syndrome. Nutrients, 9(11), 1215.

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Serum Cytokine Levels in Natalizumab Treatment

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Blood samples were taken on day 0 before natalizumab administration and 7 days afterwards (day 7). Blood was collected into serum tubes and transferred to the laboratory for storage within two hours. Serum samples were tested for concentrations of IL-6, IFN-γ, TNF-α. Ultrasensitive ELISA kits BMS213HS, BMS228HS and BMS223HS (Thermo Fisher) were used for quantitative detection of serum IL-6, IFN-γ and TNF-α respectively on both time points, following manufacturer's instructions. The detection limit that was used for statistical analysis was determined as OD blanco + SD x2, which is < 0.14 pg/ml for IFN-γ, < 0.16 pg/ml for IL-6 and < 1.25 pg/ml for TNF-α). Serum concentrations below the proposed detection limit were replaced by the arbitrary value determined as (OD blanco + SDx2)/2.

Cathérine D., Annelien P., Anne S., Luc A., Liesbeth V.H., Gerlo S, & Guy L. (2020). End of dose interval symptoms in patients treated with natalizumab: A role for serum cytokines?. Multiple sclerosis and related disorders, 41.

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