Blood samples were collected while the participants were resting in a supine position. Pre-chilled, EDTA vials (Terumo EDTA K-3) were used. The vials were placed on ice and centrifuged for 10 min at 3000× g, +4 °C, and the plasma was immediately transferred to storage at −70 °C until further analysis. No sample was thawed more than once.
Edta k 3
Manufactured by Terumo
Sourced in Japan
EDTA K-3 is a laboratory reagent used as an anticoagulant in blood sample collection. It prevents blood clotting by binding to calcium ions. EDTA K-3 is a common additive in blood collection tubes to facilitate analysis and processing of blood samples.
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Lab products found in correlation
8 protocols using edta k 3
1
Blood Sample Collection and Storage
2
Plasma Lipid and Glucose Analysis
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Blood samples were taken after overnight fasting and collected in prechilled plastic Vacutainer tubes (Terumo EDTA K-3). Plasma was prepared by centrifugation at 3000 g for 10 min at 4 °C. Blood and plasma were stored at − 70 °C pending analysis. Plasma levels of fasting glucose, low-density lipoprotein (LDL) and high-density lipoprotein (HDL) were measured [20 (link), 21 ].
3
Plasma LRP1 Quantification Protocol
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All blood samples (20 ml) were obtained while the patients were at rest in a supine position and all samples were collected in pre-chilled plastic Vacutainer tubes (Terumo EDTA K-3); however, 2 ml was used for subsequent analysis. Plasma was prepared by centrifugation at 3,000 x g for 10 min at 4˚C. All samples were stored at -70˚C until used for analysis. None of the samples were thawed more than twice.
Plasma levels of LRP1 were measured using a commercially available ELISA kit (Cloud-Clone Corporation, Houston, TX, USA) following the manufacturer's protocol. Plasma samples were diluted 1:2,000.
Plasma levels of LRP1 were measured using a commercially available ELISA kit (Cloud-Clone Corporation, Houston, TX, USA) following the manufacturer's protocol. Plasma samples were diluted 1:2,000.
4
Plasma TSP-1 Expression Analysis
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All blood samples were obtained at the start of the study, while the patients were at rest in a supine position, and all samples were collected in pre-chilled plastic Vacutainer tubes (Terumo EDTA K-3). Plasma was prepared by centrifugation at 3000 g for 10 min at 4 °C. All samples were stored at -70 °C until analysis. None of the samples were thawed more than twice. ELISA (# DTSP10, Bio-Techne, RnD systems, USA) was used to assess TSP-1 expression in plasma. In brief, levels were determined from a standard curve with absorbance read at 450 nm with wave-length correction at 540 nm.
5
Blood Sampling and Plasma Preparation
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Blood samples were collected after an overnight fast in prechilled plastic Vacutainer tubes (Terumo EDTA K-3, Tokyo, Japan). Plasma was prepared by centrifugation at 3000× g for 10 min at 4 °C. Blood and plasma were stored at – 70 °C until analysis in the chemistry laboratories at Linköping University and Ryhov County Hospitals, Jönköping. Both laboratories are ISO/IEC 17025 accredited by the Swedish Board for Accreditation and Conformity Assessment.
6
Plasma CD93 Quantification Protocol
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The blood samples were obtained while the patients were at rest in a supine position, and all samples were collected in pre-chilled plastic Vacutainer tubes (Terumo EDTA K-3). Plasma was prepared by centrifugation at 3,000 × g for 10 min at 4°C. All samples were stored at −70°C until analysis. None of the samples was thawed more than twice. Determination of CD93 levels in plasma was performed according to the recommendations (# DCD930, R&D Systems Europe, Ltd.) and as previously described (7 (link)).
7
Measurement of Plasma IL-32 Levels
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All blood samples were obtained at the start of the study, while the patients were at rest in a supine position, and all samples were collected in pre-chilled plastic Vacutainer tubes (Terumo EDTA K-3). Plasma was prepared by centrifugation at 3,000 x g for 10 min at 4˚C. All samples were stored at -70˚C until analysis. None of the samples were thawed more than twice.
Plasma IL-32 levels were measured using a commercially available enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems) following the manufacturer's protocol. According to the product manual, the kit recognizes human IL-32α, IL-32β and IL-32γ. The plasma IL-32 protein concentration from the patients and control subjects was expressed as pg/ml and all measurements including plasma and standard solutions for IL-32 standard curves were performed in duplicate.
Plasma IL-32 levels were measured using a commercially available enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems) following the manufacturer's protocol. According to the product manual, the kit recognizes human IL-32α, IL-32β and IL-32γ. The plasma IL-32 protein concentration from the patients and control subjects was expressed as pg/ml and all measurements including plasma and standard solutions for IL-32 standard curves were performed in duplicate.
8
Plasma NT-proBNP Measurement Protocol
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All blood samples were obtained while the patients were at rest in a supine position, and all samples were collected in pre-chilled plastic Vacutainer tubes (Terumo EDTA K-3). Plasma was prepared by centrifugation at 3000 g for 10 min at 4 °C. All samples were stored at −70 °C until analysis. None of the samples were thawed more than twice.
NT-proBNP was measured in the Elecsys 2010 platform (Roche Diagnostics, Mannheim, Germany). The total CV was 4.8 % at 26 pmol/L and 2.1 % at 503 pmol/L (n = 70) at our laboratory.
NT-proBNP was measured in the Elecsys 2010 platform (Roche Diagnostics, Mannheim, Germany). The total CV was 4.8 % at 26 pmol/L and 2.1 % at 503 pmol/L (n = 70) at our laboratory.
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