At the experimental day, a dummy cannula was carefully inserted and retracted into the guide to remove clotted blood and hamper spreading depression within NAc shell. One hour later, a cannula delivered the drug into the NAc shell (Table
Xylocain
Xylocain is a local anesthetic used to temporarily numb a specific area of the body. It contains the active ingredient lidocaine, which blocks the transmission of pain signals from the treated area to the brain.
Lab products found in correlation
25 protocols using xylocain
Neuropharmacological Intervention in Rats
At the experimental day, a dummy cannula was carefully inserted and retracted into the guide to remove clotted blood and hamper spreading depression within NAc shell. One hour later, a cannula delivered the drug into the NAc shell (Table
Muscle Biopsy Sampling Protocol
Muscle Biopsy Procedure for Protein Analysis
Rats: Only white vastus lateralis muscles (fast twitch fibres) were used for the present experiments, but it has previously been demonstrated that purinergic activation of the Na,K-ATPase exists in slow twitch muscles [4] (link).
Vastus Lateralis Muscle Biopsy
Neuronal Activity Modulation in Temporal Muscle
Viral Injections in Mouse Hippocampus
Bovine Mammary Tissue Biopsy
Subcutaneous Fat Metabolism Assay
Cranial Window Surgery in Mice
Alzet Pump Implantation for PEDF Delivery
We implanted the cannula 0.2 mm to the right from Bregma corresponding to the location of the right ventricle. A drop of cyanoacrylate clay (Weicon, Germany) was introduced between the cannula cap and the skull. We slowly moved the cannula down until the cap touched the skull. We left the animals for 5 min to ensure drying of the clay. Then, we carefully sutured the skin above the cannula with a 5–0 polypropilen thread (Ethicon, USA).
Animals received lidocaine gel (Xylocain, AstraZeneca, Germany) locally and 0.5 ml of ringer lactate solution (B Braun Melsungen, Germany) intraperitoneally to substitute the liquid loss. We placed the cage with operated animals onto the 37°C warm bed for 2 hours.
The animals received a total amount of 84±16.8 µl of PEDF (20 µg/ml in CSF) or CSF at a pumping rate of 0.5±0.1 µl/hour.
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