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2 protocols using boron trifluoride methanol

1

Comprehensive Chemical Reagent Procurement

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Sodium chloride, methanol, ethanol, amino acid standards, acetonitrile, phenyl isothiocyanate, gallic acid, triethylamine, n-hexane, boron trifluoride-methanol, dichloromethane, formic acid, diethyl ether, petroleum ether, sodium hydroxide, potassium hydroxide, hydrochloric acid, and perchloric acid were purchased from ANPEL Laboratory Technologies (Shanghai) Inc. Vitamin B1/B2 and vitamin E were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. Folin-Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), mixed standards of fatty acids, cyanidin 3-O-glucoside, anthranone, 1,2,3-trihydroxybenzene, benzoyl-dl-arginine p-nitroanilide hydrochloride, dimethyl sulfoxide, sulfosalicylic acid, trypsin, and phytic acid were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). The purity of chemicals used for High-Performance Liquid Chromatography (HPLC) and Gas Chromatography Mass Spectroscopy (GCMS) complied with the requirement of relevant procedures. All other chemicals were of analytical grade.
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2

Fatty acid profiling of fish roes

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Roe samples were collected from fish farms and supermarkets in China during the spawning seasons in 2018, The roes of Salmon solar, Exocoetidae cypselurus, and Pseudosciaena crocea were obtained from Fujian province, sturgeon roes (Acipenser baerii, Acipenser gueldenstaedti, Acipenser schrenckii, and Huso dauricus) were obtained from Zhejiang province. The maturity of the roes is between in the end of Ⅲ and in the beginning of Ⅳ .
Samples were transported to the laboratory on ice and stored at -18℃ until use. Boron trifluoride-methanol (14%, w/w) and n-hexane were HPLC grade, and were obtained from ANPEL Laboratory Technologies Inc., Shang-Hai, China. A thirty-seven fatty acid methyl ester (FAME) mix (NU-CHEK, USA) was used for standardization.
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