N acetyl l cysteine

Manufactured by Fujifilm

Sourced in Japan

N-acetyl-L-cysteine is a chemical compound that acts as a precursor to the antioxidant glutathione. It is used in various laboratory applications, including as a reducing agent and for protein purification.

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Lab products found in correlation

Cell counting kit 8 (cck8), by Dojindo Laboratories (2 mentions) Bafilomycin a1, by Fujifilm (2 mentions) Menadione, by Nacalai Tesque (1 mentions) Camptothecin, by Fujifilm (1 mentions) Nocodazole, by Fujifilm (1 mentions) Dulbecco s modified eagle s medium nutrient mixture f 12 ham dmem f 12, by Merck Group (1 mentions) Dulbecco s phosphate buffered saline, by Nacalai Tesque (1 mentions) Paraformaldehyde phosphate buffer solution, by Fujifilm (1 mentions) Cytotoxicity detection kitplus ldh, by Roche (1 mentions) Fxcycle pi rnase staining solution, by Thermo Fisher Scientific (1 mentions) Cellrox, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Nacalai Tesque (1 mentions) Fetal bovine serum (fbs), by Equitech-Bio (1 mentions) Dl hcy, by Merck Group (1 mentions) Trichloroacetic acid, by Fujifilm (1 mentions) L cys, by Merck Group (1 mentions) L gsh, by Merck Group (1 mentions) Cysgly, by Merck Group (1 mentions) γ glucys, by Merck Group (1 mentions) Ammonium 7 fluoro 2 1 3 benzoxadiazole 4 sulfonate sbd f, by Dojindo Laboratories (1 mentions)

Close spelling variants of this product

L-cysteine (15 citations) N-acetyl-L-cysteine (NAC) (12 citations) Cysteine (9 citations)

14 protocols using n acetyl l cysteine

Cytotoxicity and Apoptosis Assays in Cell Culture

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Dulbecco’s modified eagle’s medium nutrient mixture F-12 HAM (DMEM/F-12) was obtained
from SIGMA (Tokyo, Japan). Fetal bovine serum (FBS) was purchased from Equitech-Bio, Inc.
(Kerrville, TX, U.S.A.). Penicillin/streptomycin, Dulbecco’s phosphate-buffered saline
(PBS) and menadione were purchased from Nacalai tesque (Kyoto, Japan). Camptothecin,
nocodazole, 4% paraformaldehyde phosphate buffer solution (4% PFA), N-acetyl-l-cysteine
(NAC), catalase, mercury (II) chloride (HgCl₂), hydrogen peroxide
(H₂O₂) were from Wako (Osaka, Japan), FxCycle PI/RNase staining
solution and CellROX from Thermo Fisher Scientific (Carlsbad, CA, U.S.A.) and Accumax from
Innovative Cell Technologies (San Diego, CA, U.S.A.). Methylmercury (II) chloride standard
(MeHg) and cadmium chloride 2.5-hydrate (CdCl₂) were obtained from Kanto
Chemical (Tokyo, Japan). Cell counting Kit-8 was from Dojindo (Kumamoto, Japan).
Cytotoxicity detection KitPLUS (LDH) and Cell proliferation ELISA, BrdU (colorimetric)
were purchased from Roche (Basel, Switzerland). Amplite fluorimetric Caspase 3/7 assay kit
was from AAT Bioquest (Sunnyvale, CA, U.S.A.).

SUDO K., VAN DAO C., MIYAMOTO A, & SHIRAISHI M. (2019). Comparative analysis of in vitro neurotoxicity of methylmercury, mercury, cadmium, and hydrogen peroxide on SH-SY5Y cells. The Journal of Veterinary Medical Science, 81(6), 828-837.

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Quantification of Thiol Compounds

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l-Cys, D,l-Hcy, l-GSH, CysGly, and γGluCys were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-Acetyl-l-Cysteine (NAC), tiopronin (N-(2-mercaptopropionyl)glycine, MPG), and trichloroacetic acid (TCA) were obtained from Wako (Osaka, Japan). Ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) was purchased from Dojindo (Kumamoto, Japan). TCEP was from Tokyo Chemical Industry (Tokyo, Japan). Phosphate buffered saline (PBS) was purchased from Takara Bio (Shiga, Japan). HPLC-grade acetonitrile was used. Water was purified using a Milli-Q system (Millipore, Bedford, MA, USA). All other chemicals were of analytical grade.

Chang C., Isokawa M., Funatsu T, & Tsunoda M. (2019). Optimization of tris(2-carboxyethyl) phosphine reduction conditions for fast analysis of total biothiols in mouse serum samples. Heliyon, 5(5), e01598.

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Antioxidant Effects on Cell Viability

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Cells were cultured in RPMI 1640 supplemented with 10% fetal bovine serum and 1% chicken serum at 39 °C under 5% CO₂ without a reductant for a normal culture [22 (link)]. To investigate the effects of various antioxidants on cell viability, cells were cultured in the presence or absence of DOX for the indicated periods and then treated with antioxidants, 200 μM ascorbic acid phosphate magnesium salt (Wako, Osaka, Japan), 5 mM N-acetyl-L-cysteine (NAC, Wako, Osaka, Japan), 250 μM 1,2-dihydroxy-3,5-benzenedisulfonic acid (Tiron, Sigma-Aldrich), or 50 μM 2-mercaptoethanol (Et-SH, Sigma-Aldrich). Growth curves were generated as previously described [19 (link)–21 (link)].

Moriwaki T., Yoshimura A., Tamari Y., Sasanuma H., Takeda S., Seki M, & Tano K. (2021). PRDX1 is essential for the viability and maintenance of reactive oxygen species in chicken DT40. Genes and Environment, 43, 35.

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3D Culture of PDAC Organoids

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Five patient-derived PDAC organoids (KYK070, KYK002, KYK023, KYK090 and KYK093) were cultured three-dimensionally in growth factor reduced Matrigel (354230; Corning, Corning, NY, USA) with complete organoid media containing advanced DMEM/F12 (12634-010; Life Technologies) supplemented with 10% Afamin/Wnt3a CM (J2-001; Medical & Biological Laboratories), 10% R-spondin1-conditioned medium from Cultrex R-spondin1 Cell and Reagent (3710-001-01; R&D Systems, Minneapolis, MN, USA), 10 mM HEPES (15630-080; Life Technologies), 1% GlutaMax (35050-061; Life Technologies), 2% B27 (17504044; Life Technologies), 10 nM gastrin-I (G9020-1MG; Merck KGaA), 500 mM N-acetyl-L-cysteine (017-05131; FUJIFILM Wako Pure Chemical Corporation), 10 ng/ml EGF (236-EG; R&D systems), 100 ng/ml noggin (6057-NG; R&D Systems), 1 mM A83-01 (SML0788-5MG; Merck KGaA), 100 ng/ml FGF-10 (060-04401; FUJIFILM Wako Pure Chemical Corporation) and 10 mM nicotinamide (N0636; Merck KGaA). Media were replaced every two to three days. All organoids were maintained at 37 °C in a humidified atmosphere containing 5% CO₂.

Yamakawa K., Koyanagi-Aoi M., Machinaga A., Kakiuchi N., Hirano T., Kodama Y, & Aoi T. (2023). Blockage of retinoic acid signaling via RARγ suppressed the proliferation of pancreatic cancer cells by arresting the cell cycle progression of the G1-S phase. Cancer Cell International, 23, 94.

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Apoptosis Induction Reagents

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Rap and Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD-FMK) were purchased from Medical and Biological Laboratories (MBL, Nagoya, Japan). N-acetyl-L-cysteine and 3-methyladenine were purchased from Wako Pure Chemical Industries (Osaka, Japan). Doxorubicin was purchased from Sigma.

Ono K., Sato T., Iyama S., Tatekoshi A., Hashimoto A., Kamihara Y., Horiguchi H., Kikuchi S., Kawano Y., Takada K., Hayashi T., Miyanishi K., Sato Y., Takimoto R., Kobune M, & Kato J. (2014). A novel strategy inducing autophagic cell death in Burkitt's lymphoma cells with anti-CD19-targeted liposomal rapamycin. Blood Cancer Journal, 4(2), e180-.

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Quantitative Analysis of Sphingolipid Metabolites

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D-erythro-sphinganine-D₇ ([D₇]d18:0), D-erythro-sphingosine-D₇ ([D₇]d18:1), and N-palmitoyl [D₃₁]-D-erythro-sphingosine (d18:1-[D₃₁]C16:0-Cer) as internal standards (ISs) labeled with stable isotopes were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL). [(2S,3S)-3-propylcarbamoyloxirane-2-carbonyl]-L-isoleucyl-L-proline methyl ester (CA-074Me as an inhibitor of cathepsin B), N-acetyl-L-cysteine (NAC as an inhibitor of oxidative stress), 3,6-Bis(dimethylamino) acridine hydrochloride (acridine orange) solution (1 mg/mL water), 10% ammonia aqueous solution, cholesterol, dithiothreitol, and lithium dodecyl sulfate were purchased from Wako (Osaka, Japan). D-NMAPPD as an inhibitor of ceramidase was purchased from Cayman Chemical (Ann Arbor, MI). DL-PDMP was obtained from Biomol Research Laboratories (Plymouth Meeting, PA). β-cholestanol was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).

Yamane M., Moriya S, & Kokuba H. (2017). Visualization of ceramide channels in lysosomes following endogenous palmitoyl-ceramide accumulation as an initial step in the induction of necrosis. Biochemistry and Biophysics Reports, 11, 174-181.

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Modulating Signaling Pathways in Cell Cultures

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SB (cat no. 193-01152; reagent grade), trichostatin A (TSA; cat. no. 203-17561), methotrexate (cat. no. 139-13571) and N-acetyl-L-cysteine (NAC; cat. no. 015-05132; ROS scavenger) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Anti-focal adhesion kinase (FAK) polyclonal antibody (cat no. sc-1688) and antibodies against p16 (cat no. sc166760), p21 (cat no. sc-166630), p27 (cat no. sc-1641), p53 (cat no. sc-47698), SIRT1 (cat no. sc-74504) MLC20 (cat no. sc-9449) and β-actin (cat no. sc-47778) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Anti-phospho (p)-tyrosine pY397-FAK (cat no. 44-624G) and pY577-FAK (cat no. 44-614G) polyclonal antibodies were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Anti-p-myosin light chain 20 (p-MLC20) antibody was provided by Dr Fumio Matsumura (Rutgers University, New Brunswick, NJ, USA). All other chemicals used were commercial products from Wako Pure Chemical Industries, Ltd. SB (200 mM stock) and TSA (1 mM stock) were dissolved in phosphate-buffered saline (PBS) and diluted in the cell media prior to use.

Nakagawa H., Sasagawa S, & Itoh K. (2017). Sodium butyrate induces senescence and inhibits the invasiveness of glioblastoma cells. Oncology Letters, 15(2), 1495-1502.

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Inhibitor Compounds for Cell Death Pathways

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Lapatinib, FTY720, hydroxychloroquine (HCQ), U18666A and gefitinib were purchased from Cayman Chemical Company and dissolved in dimethyl sulfoxide (DMSO) at a concentration of 20 mM to obtain stock solutions. Abemaciclib and z-VAD-FMK, pan-caspase inhibitors, were purchased from AdooQ BioScience. E64d and pepstatin A, inhibitors of lysosomal proteases, were purchased from Peptide Institute, Inc. Necrostatin-1, a specific inhibitor of receptor-interacting serine/threonine-protein kinase 1 (RIPK1), was purchased from Enzo Life Sciences, Inc., and N-acetyl-L-cysteine, staurosporine, bafilomycin A₁ and Hanks' balanced salt solution (HBSS) were obtained from FUJIFILM Wako Pure Chemical Corporation. Hoechst 33342 was obtained from Nacalai Tesque, Inc., and DAPI was purchased from Sigma-Aldrich (Merck KGaA).

Suzuki S., Ogawa M., Miyazaki M., Ota K., Kazama H., Hirota A., Takano N., Hiramoto M, & Miyazawa K. (2021). Lysosome-targeted drug combination induces multiple organelle dysfunctions and non-canonical death in pancreatic cancer cells. Oncology Reports, 47(2), 40.

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Cellular Stress and Autophagy Modulation

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6-hydroxydopamine hydrochloride (6-OHDA; H4381), catalase (C8531), rotenone (R8875), MRT68921 (SML1644), and trehalose (T9531) were obtained from Sigma-Aldrich. Bafilomycin A₁ (023–11641), benzoquinone (171–00242), H₂O₂ (080–01186), N-acetyl-L-cysteine (NAC; 017–05131), and Z-VAD(OMe)-FMK (zVAD; 269–02071) were from Wako. Brefeldin A (203729) was from Merck. Ac-YVAD-CMK (YVAD; 10014) was from Cayman Chemical Company. Rapamycin (S1039) was obtained from Selleck Chemicals. The following antibodies were from commercial sources: anti-PARK7/DJ-1 (ab4150), anti-CTSB (abcam, ab58802); anti-RPN1 (Santa Cruz Biotechnology, sc-12164); anti-FN1 (610077), anti-CAV1 (BD Biosciences, 610060); anti-VDAC1 (Merck, AP1059); anti-LMNA (2032), anti-CASP3 (9662), anti-ATG16L1 (8089), anti-phospho-AMPK Thr172 (2535), anti-phospho-ULK1 Ser555 (5869), anti-phospho-RPS6KB1/p70S6K Thr389 (Cell Signaling Technology, 9234); and anti-LC3B (L7543), and anti-ACTB (Sigma-Aldrich, A5441). Monoclonal antibody against PARK7 (Clone 3843) was prepared as previously described [62]. All other chemicals, of analytical grade, were obtained from Sigma-Aldrich or Wako.

Urano Y., Mori C., Fuji A., Konno K., Yamamoto T., Yashirogi S., Ando M., Saito Y, & Noguchi N. (2018). 6-Hydroxydopamine induces secretion of PARK7/DJ-1 via autophagy-based unconventional secretory pathway. Autophagy, 14(11), 1943-1958.

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Cell Viability and Oxidative Stress Assay Protocol

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The Cell Counting Kit-8 and Diphenyl-1-pyrenylphosphine (DPPP) were purchased from Dojindo (Tokyo, Japan), anti-monocarboxylate transporter 1 (MCT1) antibodies were obtained from Alpha Diagnostic International, Inc. (San Antonio, TX), and anti-caspase 9 antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA). Radioisotope of [2-¹⁴C]-Acetic acid sodium salt was purchased from American Radiolabeled Chemicals, Inc. (St. Louis, MO). Acetic acid, hydrochloric acid and N-Acetyl-l-cysteine (NAC) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The 12% Bis Tris gels were obtained from Life Technologies Japan, Ltd. (Tokyo, Japan), and the Can Get Signal reagents were obtained from TOYOBO Co., Ltd. (Osaka, Japan). 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) was purchased from LABOTEC Co., Ltd. (Tokyo, Japan).

Terasaki M., Ito H., Kurokawa H., Tamura M., Okabe S., Matsui H, & Hyodo I. (2018). Acetic acid is an oxidative stressor in gastric cancer cells. Journal of Clinical Biochemistry and Nutrition, 63(1), 36-41.

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