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Apoe 75 85

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The ApoE 75-85 is a peptide that corresponds to the 75-85 amino acid region of the Apolipoprotein E (ApoE) protein. ApoE is a key regulator of lipid metabolism and has been implicated in various biological processes. The ApoE 75-85 peptide can be used in research applications, but a detailed and unbiased description of its core function cannot be provided without the risk of extrapolation.

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Lab products found in correlation

Anti apoe, by Merck Group (2 mentions) Recombinant apoe isoforms, by BioCat (2 mentions) Apoe3, by Romer Labs (2 mentions) Apoe 139 152, by Cell Biolabs (2 mentions) Apoe 210 232, by Cell Biolabs (2 mentions) Mda ldl, by Cell Biolabs (2 mentions) Copper oxidized ldl oxldl, by Thermo Fisher Scientific (2 mentions) Anti c5b 9 clone ae11, by Agilent Technologies (2 mentions) Anti aβ clone32a1, by AbFrontier (2 mentions)

2 protocols using apoe 75 85

1

Characterization of Complement-ApoE Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Complement components C2, C3, C3b, C4, C4b, C1q, C1s, Factor H, Factor
I, and C4BP as well as all primary antibodies (anti C1q, A200/3b; anti C2,
A212/18b; anti C3, A213/5a; anti C4, A201/3a; anti factor H, A237/4) were
purchased from Complement Technology. Recombinant ApoE isoforms from BioCat;
plasma-purified ApoE3 from Biopure. ApoE peptides were generated by Peptide 2.0:
ApoE 30-40 LGRFWDYLRWV; ApoE 75-85 YKSELEEQLTPV; ApoE 139-152 SHLRKLRKRLLRDA;
ApoE 210-232 WGERLRARMEEMGSRTRDRLDEV. LDL and malondialdehyde-modified LDL
(MDA-LDL) were from Cell Biolabs, copper oxidized LDL (oxLDL) from Thermo Fisher
(L34357), ApoA from Athens Research&Technology, vitronectin (Vnt) from
Corning. Aβ and Aβ fibrils from GenSript. Recombinant EfB was
expressed as described65 . Additional
antisera used were: anti-C5b-9 (clone AE11-MO777-Dako), anti C1s
(11951-05011-AssaybioTch), anti ApoE ((178479-Calbiochem), anti ApoE (Merck,
178479), anti LDLR (HB04JL2204-B-SinoBiological), anti Aβ (clone
32A1-Abfrontier), anti goat IgG (13C0836-Sigma Aldrich) and anti rabbit IgG
(13C0529-Sima Aldrich), IgM (120228-Jackson ImmunoResearch Laboratories).
Yin C., Ackermann S., Ma Z., Mohanta S.K., Zhang C., Li Y., Nietzsche S., Westermann M., Peng L., Hu D., Bontha S.V., Srikakulapu P., Beer M., Megens R.T., Steffens S., Hildner M., Halder L.D., Eckstein H.H., Pelisek J., Herms J., Roeber S., Arzberger T., Borodovsky A., Habenicht L., Binder C.J., Weber C., Zipfel P.F., Skerka C, & Habenicht A.J. (2019). ApoE attenuates unresolvable inflammation by complex formation with activated C1q. Nature medicine, 25(3), 496-506.
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2

Characterization of Complement-ApoE Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Complement components C2, C3, C3b, C4, C4b, C1q, C1s, Factor H, Factor
I, and C4BP as well as all primary antibodies (anti C1q, A200/3b; anti C2,
A212/18b; anti C3, A213/5a; anti C4, A201/3a; anti factor H, A237/4) were
purchased from Complement Technology. Recombinant ApoE isoforms from BioCat;
plasma-purified ApoE3 from Biopure. ApoE peptides were generated by Peptide 2.0:
ApoE 30-40 LGRFWDYLRWV; ApoE 75-85 YKSELEEQLTPV; ApoE 139-152 SHLRKLRKRLLRDA;
ApoE 210-232 WGERLRARMEEMGSRTRDRLDEV. LDL and malondialdehyde-modified LDL
(MDA-LDL) were from Cell Biolabs, copper oxidized LDL (oxLDL) from Thermo Fisher
(L34357), ApoA from Athens Research&Technology, vitronectin (Vnt) from
Corning. Aβ and Aβ fibrils from GenSript. Recombinant EfB was
expressed as described65 . Additional
antisera used were: anti-C5b-9 (clone AE11-MO777-Dako), anti C1s
(11951-05011-AssaybioTch), anti ApoE ((178479-Calbiochem), anti ApoE (Merck,
178479), anti LDLR (HB04JL2204-B-SinoBiological), anti Aβ (clone
32A1-Abfrontier), anti goat IgG (13C0836-Sigma Aldrich) and anti rabbit IgG
(13C0529-Sima Aldrich), IgM (120228-Jackson ImmunoResearch Laboratories).
Yin C., Ackermann S., Ma Z., Mohanta S.K., Zhang C., Li Y., Nietzsche S., Westermann M., Peng L., Hu D., Bontha S.V., Srikakulapu P., Beer M., Megens R.T., Steffens S., Hildner M., Halder L.D., Eckstein H.H., Pelisek J., Herms J., Roeber S., Arzberger T., Borodovsky A., Habenicht L., Binder C.J., Weber C., Zipfel P.F., Skerka C, & Habenicht A.J. (2019). ApoE attenuates unresolvable inflammation by complex formation with activated C1q. Nature medicine, 25(3), 496-506.
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