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Icap 6300 duo view spectrometer

Manufactured by Thermo Fisher Scientific
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The ICAP 6300 Duo View Spectrometer is a versatile laboratory instrument designed for elemental analysis. It utilizes Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) technology to detect and quantify a wide range of elements in various sample types. The instrument features a Duo View configuration, allowing for both axial and radial plasma viewing to optimize performance across a broad concentration range.

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Lab products found in correlation

Jem 2100f transmission electron microscope, by JEOL (2 mentions) Xpert pro mpd x, by Philips (2 mentions) Jobin yvon fluorolog3 spectrometer, by Horiba (2 mentions) Jem 2100f, by JEOL (2 mentions) Zetasizer nano zs90, by Malvern Panalytical (2 mentions) Jsm 7001f, by JEOL (1 mentions) Jem 2100, by JEOL (1 mentions) Cm200, by Philips (1 mentions) D max 2200, by Rigaku (1 mentions) X maxn, by Oxford Instruments (1 mentions) Cary 6000i uv vis nir spectrophotometer, by Agilent Technologies (1 mentions) Microtof q 2, by Bruker (1 mentions) Xl30 sirion, by Thermo Fisher Scientific (1 mentions) Tecnai g2 f20 x twin, by Thermo Fisher Scientific (1 mentions) Asap 2020, by Micromeritics (1 mentions) Amicon ultra 30 k filters, by Merck Group (1 mentions)

Spelling variants of this product

ICAP 6300 (138 citations) ICAP 6300 Duo (54 citations) ICAP 6300 Duo VIEW ICP Spectrometer (4 citations)

13 protocols using icap 6300 duo view spectrometer

1

Ytterbium Quantification in Tissues and Feces

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Tissue and feces samples were digested in concentrated nitric acid (68%) for overnight, followed by heating in concentrated nitric acid and hydrogen peroxide for 2 hours using a hot plate, to obtain clear solutions. The Yb contents in the solutions were determined by ICP-OES (Thermo Scientific ICAP 6300 Duo View Spectrometer), and the Yb contents in the mice tissues and feces were subsequently calculated.
Zhong Y., Ma Z., Wang F., Wang X., Yang Y., Liu Y., Zhao X., Li J., Du H., Zhang M., Cui Q., Zhu S., Sun Q., Wan H., Tian Y., Liu Q., Wang W., Garcia K.C, & Dai H. (2019). In vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles. Nature biotechnology, 37(11), 1322-1331.
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2

Multimodal Characterization of Nanomaterials

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Transmission electron microscopy (TEM) images were taken with a JEM-2100F transmission electron microscope (JEOL) operating at 200 kV. Energy dispersive X-ray (EDX) mapping images were obtained on a JEM-2100F equipped with an energy dispersive X-ray analyzer. Inductively coupled plasma optical emission spectrometry (ICP-OES) were performed on a Thermo Scientific ICAP 6300 Duo View Spectrometer. Dynamic light scattering (DLS) and zeta potential measurements were performed on a Malvern Zetasizer Nano ZS90. X-ray diffraction (XRD) patterns were recorded on a Philips XPert PRO MPD X-ray diffractometer operated at 35 kV and 45 mA with Cu-Kα radiation. The upconversion luminescent properties were studied using a Horiba Jobin Yvon FluoroLog3 spectrometer equipped with a 980 nm diode laser as excitation. The downconversion luminescent properties were studied using an Acton SP2300i spectrometer equipped with an InGaAs linear array detector (Princetion OMA-V) and using a 980 nm diode laser as excitation. NIR fluorescence images of the downconversion emission were obtained using 2D InGaAs array (Ninox 640, Raptor Photonics) with 640 × 512 pixel using a 980 nm diode laser as excitation. Raman spectra were obtained with polarized incident laser light (λ = 532 nm) on Jobin Yvon T64000.
Zhong Y., Ma Z., Wang F., Wang X., Yang Y., Liu Y., Zhao X., Li J., Du H., Zhang M., Cui Q., Zhu S., Sun Q., Wan H., Tian Y., Liu Q., Wang W., Garcia K.C, & Dai H. (2019). In vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles. Nature biotechnology, 37(11), 1322-1331.
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3

Elemental Analysis of Cleared Brain Tissue

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Four hydrogel-embedded but uncleared samples and four cleared brain samples were measured with ICP-OES to determine iron, calcium and phosphorus content before and after clearing. The samples were cut into small pieces, dissolved in 50μl concentrated HNO3 and heated to 130°C for 12 hours. When all the liquid had evaporated, the samples were re-suspended in 5 ml 2% HNO3. Standard solutions for iron with 5ppm, 1ppm, 0.5ppm, 0.1ppm, 0.01ppm concentrations, for calcium with 5ppm, 1ppm, 0.5ppm, 0.1ppm, 0.01ppm and for phosphorus with 20ppm, 5ppm, 1ppm, 0.5ppm, 0.01ppm were prepared. ICP-OES was performed using a Thermo Scientific ICAP 6300 Duo View Spectrometer.
Leuze C., Aswendt M., Ferenczi E., Liu C.W., Hsueh B., Goubran M., Tian Q., Steinberg G., Zeineh M.M., Deisseroth K, & McNab J.A. (2017). The Separate Effects of Lipids and Proteins on Brain MRI Contrast Revealed Through Tissue Clearing. NeuroImage, 156, 412-422.
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4

Zinc Quantification in Mouse Islets

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Isolated mouse islets, mouse exocrine tissue, and trypsinized R7T1 and HEK 293T cells were centrifuged at 800 rpm for 5 min. Samples were digested by adding concentrated HNO3 (228 uL) and heating samples 30 s at 100 °C, t hen freezing at −20 °C. On the day of the experiment, samples were thawed and diluted to 2% HNO3. An aliquot (10 uL) was taken and neutralized with 2 N NaOH (10 uL), and the concentration of protein was determined by DCA protein assay. The remainder of the solution was diluted to a final concentration of 2% HNO3 (8 mL) and the amount of zinc was determined by ICP-OES (Inductively coupled plasma - optical emission spectrometry) on a Thermo Scientific ICAP 6300 Duo View Spectrometer, running a diluted zinc standard in parallel to convert responses to concentrations. Data are represented as zinc normalized to total protein.
Horton T.M., Allegretti P.A., Lee S., Moeller H.P., Smith M, & Annes J.P. (2018). Zinc-Chelating Small Molecules Preferentially Accumulate and Function within Pancreatic β-Cells. Cell chemical biology, 26(2), 213-222.e6.
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5

Comprehensive Characterization of G-Se Microballs

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The microstructure of the microballs was examined by SEM (JSM-7001F, JEOL, Ltd.), TEM (CM200, Philips) and HR-TEM (JEM-2100, JEOL, Ltd.), while elemental mapping was carried out by EDX (X-MaxN, Oxford instruments). XRD (DMAX-2200, Rigaku) patterns were recorded at room temperature using Cu-Kα radiation (λ = 1.54056 Å) at a scan rate of 1° min−1, and the scans were performed at 0.04° intervals for 2θ values of 5°–80°. In addition, XPS measurements were conducted using an Omicron ESCA Probe (Omicron Nanotechnology) with monochromated Al-Ka radiation (hν = 1486.6 eV). Raman spectra (Jobin-Yvon LabRAM HR) were recorded at room temperature utilizing a conventional backscattering geometry and a liquid-nitrogen-cooled charge-coupled device multichannel detector. An argon-ion laser with a wavelength of 514.5 nm was utilised as the light source. The thermal properties of the G–Se hybrid microballs were determined using a thermogravimetric analyser (STA409 PC) under N2. Thermogravimetric analysis was carried out from room temperature to 1000 °C at a heating rate of 10 °C min−1. ICP-OES analysis was performed using a Thermo Scientific ICAP 6300 Duo View Spectrometer.
Youn H.C., Jeong J.H., Roh K.C, & Kim K.B. (2016). Graphene–Selenium Hybrid Microballs as Cathode Materials for High-performance Lithium–Selenium Secondary Battery Applications. Scientific Reports, 6, 30865.
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6

Comprehensive Materials Characterization

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X-ray diffraction measurements were carried out via a Bruker D8-Advance diffractometer using Ni filtered Cu Kα radiation. SEM images were collected on a FEI Sirion scanning electron microscope and TEM images were taken on a JEOL JEM-2010 transmission electron microscope operated at 200 kV. TG-DSC analysis was performed using Netzsch STA 449 with air flow at a heating rate of 10 °C min−1 from room temperature to 600 °C. Inductively coupled plasma mass spectrometry was carried out using a Thermo Scientific ICAP 6300 Duo View Spectrometer. (See details in Supplementary Methods).
Li S., Niu J., Zhao Y.C., So K.P., Wang C., Wang C.A, & Li J. (2015). High-rate aluminium yolk-shell nanoparticle anode for Li-ion battery with long cycle life and ultrahigh capacity. Nature Communications, 6, 7872.
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7

Biodistribution of Probes via ICP-MS

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The biodistribution of probes was analyzed 10 min, 30 min, 24 h or 48 h postadministration. The urine, feces and major organs including the liver, spleen, heart, lungs, and kidneys were collected and digested in nitric acid (68%) for 12 h. Afterwards, the solutions were heated to 150 °C in the digestion solution (nitric acid:hydrogen peroxide = 4: 1) until transparent and colorless solution was obtained. The concentration of gold in each sample was measured by ICP-MS (Thermo Scientific ICAP 6300 Duo View Spectrometer).
Baghdasaryan A., Wang F., Ren F., Ma Z., Li J., Zhou X., Grigoryan L., Xu C, & Dai H. (2022). Phosphorylcholine-conjugated gold-molecular clusters improve signal for Lymph Node NIR-II fluorescence imaging in preclinical cancer models. Nature Communications, 13, 5613.
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8

Characterization of Au-PC Conjugates

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UV-vis spectra were recorded on a Varian Cary 6000i UV/Vis/NIR spectrophotometer, using a quartz cuvette of 2 mm path length. Spectra were measured in the range of 200–1000 nm in water with a scanning speed of 200 nm min−1 with spectral bandwidth of 2 nm. The emission spectra were measure by an Acton SP2300i spectrometer equipped with an InGaAs linear array detector (Princeton OMA-V). The quantum yields were measured using integrated sphere method. ESI-MS analyses were performed on Bruker MicroTOF-Q II; the sample was introduced by syringe pump at 3 µL/min and the full scan MS spectra were collected in negative ion mode. Inductively coupled plasma mass spectrometry (ICP-MS) was performed on a Thermo Scientific ICAP 6300 Duo View Spectrometer. The Infrared spectra were measured on Nicolet iS50 FT/IR spectrometer. The PC ligand, Au-GSH cluster and Au-PC conjugate were drop casted on a diamond internal reflection element (IRE) and allowed to dry in air. IR spectra were measured in ATR mode. The spectra were recorded with a spectral resolution of 4 cm−1, in the range 400–4000 cm−1.
Baghdasaryan A., Wang F., Ren F., Ma Z., Li J., Zhou X., Grigoryan L., Xu C, & Dai H. (2022). Phosphorylcholine-conjugated gold-molecular clusters improve signal for Lymph Node NIR-II fluorescence imaging in preclinical cancer models. Nature Communications, 13, 5613.
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9

Ytterbium Quantification in Tissues and Feces

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Tissue and feces samples were digested in concentrated nitric acid (68%) for overnight, followed by heating in concentrated nitric acid and hydrogen peroxide for 2 hours using a hot plate, to obtain clear solutions. The Yb contents in the solutions were determined by ICP-OES (Thermo Scientific ICAP 6300 Duo View Spectrometer), and the Yb contents in the mice tissues and feces were subsequently calculated.
Zhong Y., Ma Z., Wang F., Wang X., Yang Y., Liu Y., Zhao X., Li J., Du H., Zhang M., Cui Q., Zhu S., Sun Q., Wan H., Tian Y., Liu Q., Wang W., Garcia K.C, & Dai H. (2019). In vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles. Nature biotechnology, 37(11), 1322-1331.
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10

Morphological and Microstructural Analysis

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The morphology and the microstructure were studied using SEM (FEI, XL30 Sirion) and TEM (FEI, Tecnai G2 F20 X-TWIN). The specific surface area was characterized from nitrogen adsorption–desorption measurement (Micromeritics, ASAP 2020). ICP-OES was conducted using a Thermo Scientific ICAP 6300 Duo View Spectrometer.
Tao X., Wang J., Liu C., Wang H., Yao H., Zheng G., Seh Z.W., Cai Q., Li W., Zhou G., Zu C, & Cui Y. (2016). Balancing surface adsorption and diffusion of lithium-polysulfides on nonconductive oxides for lithium–sulfur battery design. Nature Communications, 7, 11203.
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