Advia 1800 clinical chemistry analyzer

Laboratory measurements were performed in the central laboratory of the university hospital as ordered by attending physicians. Blood cell and platelet counts were determined on the ADVIA 2120 hematology analyzer. Semiquantitative proteinuria (“urine dipstick protein”) was determined using the iChemVELOCITY urinalysis system (Iris Diagnostics, Beckman Coulter, Krefeld, Germany). Urinary albumin levels were measured nephelometrically (BN Prospec Nephelometer). Urinary protein was determined using the benzethonium chloride method (Roche Diagnostics, Mannheim Germany) and CRP was determined using a wide-range latex-enhanced immunturbidimetric assay. Plasma and urine creatinine was measured enzymatically using the creatinase method and urinary concentrations of NGAL were determined using a particle-enhanced turbidimetric immunoassay (BioPorto Diagnostics, Gentofte, Denmark). Plasma sodium and all other measurements above were performed on an ADVIA 1800 clinical chemistry analyzer (all instruments from Siemens Healthcare Diagnostics, Eschborn Germany).
PCT concentrations were determined using the BRAHMS KRYPTOR Time Resolved Amplified Cryptate Emission Immunoassay system (Thermo Scientific, Hennigsdorf, Germany).

At enrolment (T0) and after two months (T2), routinary laboratory measurements (haemoglobin, urea, creatinine, estimated glomerular filtration rate (eGFR) according to the CKD-EPI equation [21 (link)], sodium, potassium, uric acid, calcium, phosphate, parathyroid hormone (PTH), bicarbonates and albumin) were measured on an ADVIA^® 1800 Clinical Chemistry Analyzer (Siemens Healthcare Diagnostics, Munich, Germany); total and free serum p-Cresyl Sulphate (t- and f-PCS, respectively) and total and free serum Indoxyl Sulphate (t- and f-IS, respectively) were measured by means of a high-performance liquid chromatography technique coupled with tandem mass spectrometry (B.S.N. Srl, Castelleone, Italy); serum Lp-PLA₂ activity was measured with the new PLAC^® test (Diazyme Laboratories, Inc., 12889 Gregg Court, Poway, CA, USA).
Nutritional status was assessed by physical examination, measuring body weight, height, BMI (kg/m²), dominant Hand Grip strength (kg) using Hydraulic Hand Dynamometer Owner’s Manual (Sammons Preston, Bolingbrook, IL, USA), according to the reference values [22 ,23 ]. Bioelectrical impedance analysis (BIA) was used to estimate fat-free body mass (kg), fat mass (kg) and phase angle through an Akern model 101 (Akern Srl, Pisa, Italy).

Cells were cultivated for 5 days with 0.5, 0.05 or 0.005% Lipofundin. Cell culture supernatant was removed, and after washing with PBS, cells were lysed with 500 µl H₂O and centrifuged with 13 000g for 10 min. LDH concentration was immediately measured in cell culture supernatant and lysate with a Siemens ADVIA 1800 Clinical Chemistry Analyzer.