Super-resolution 3D-SIM images were acquired on a DeltaVision OMX V4 (GE Healthcare) equipped with a 60x/1.42 NA PlanApo oil immersion objective (Olympus), 405, 488, 568 and 642 nm solid state lasers (100 mW) and sCMOS cameras (pco.edge). Image stacks of 1 µm with 0.125 µm thick z-sections and 15 images per optical slice (3 angles and 5 phases) with were acquired using immersion oil with a refractive index 1.524. Images were reconstructed using Wiener filter settings of 0.005 and optical transfer functions (OTFs) measured specifically for each channel with SoftWoRx 6.1.3 (GE Healthcare) to obtain super-resolution images with a twofold increase in resolution both axially and laterally. Images from different color channels were registered using parameters generated from a gold grid registration slide (GE Healthcare) and SoftWoRx 6.1.3 (GE Healthcare).
Gold grid registration slide
Manufactured by GE Healthcare
The Gold Grid Registration Slide is a lab equipment product designed for calibration and registration purposes. It features a precise grid pattern made of gold material. The primary function of this slide is to assist in the alignment and calibration of imaging systems used in various scientific and medical applications.
Automatically generated - may contain errors
4 protocols using gold grid registration slide
1
Super-resolution 3D-SIM Imaging Protocol
2
Superresolution Imaging Using 3D-SIM
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Superresolution 3D structured illumination microscopy (SIM) images were acquired on a DeltaVision OMX SR (GE Healthcare) equipped with a 60× 1.42 NA PlanApo oil immersion lens (Olympus); 405-, 488-, 568-, and 640-nm solid state lasers; and sCMOS cameras (pco.edge). Image stacks with 0.125-µm-thick z-sections, and 15 images per optical slice (three angles and five phases) were acquired using immersion oil with a refractive index 1.516. Images were reconstructed using Wiener filter settings of 0.003 and optical transfer functions (OTFs) measured specifically for each channel with SoftWoRx 6.5.2 (GE Healthcare) to obtain superresolution images with a twofold increase in resolution both axially and laterally. Images from different color channels were registered using parameters generated from a gold grid registration slide (GE Healthcare) and SoftWoRx 6.5.2 (GE Healthcare).
3
Super-resolution Imaging Acquisition and Analysis
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Super-resolution 3D-SIM images were acquired on a DeltaVision OMX V4 (GE Healthcare) equipped with a 60×/1.42 NA PlanApo oil immersion lens (Olympus), 405-, 488-, 568-, and 642-nm solid-state lasers and sCMOS cameras (pco.edge). Image stacks of 5–6 µm with 0.125-µm thick z-sections and 15 images per optical slice (3 angles and 5 phases) which were acquired using immersion oil with a refractive index 1.518. Images were reconstructed using Wiener filter settings of 0.003 and optical transfer functions measured specifically for each channel with SoftWoRx 6.1.3 (GE Healthcare) to obtain super-resolution images with a twofold increase in resolution both axially and laterally. Images from different color channels were registered using parameters generated from a gold grid registration slide (GE Healthcare) and SoftWoRx 6.1.3 (GE Healthcare).
4
Super-resolution Structured Illumination Microscopy
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Superresolution 3D structured illumination microscopy images were acquired on a DeltaVision OMX SR (GE Healthcare) equipped with a 60×/1.42 NA PlanApo oil immersion lens (Olympus), 405-, 488-, 568-, and 640-nm solid-state lasers, and sCMOS cameras (pco.edge). Image stacks of 5–6 µm with 0.125-µm-thick Z sections and 15 images per optical slice (three angles and five phases) were acquired using immersion oil with a refractive index 1.518. Images were reconstructed using Wiener filter settings of 0.003 and optical transfer functions measured specifically for each channel with SoftWoRx 6.5.1 (GE Healthcare) to obtain superresolution images with a twofold increase in resolution both axially and laterally. Images from different color channels were registered using parameters generated from a gold grid registration slide (GE Healthcare) with the routines available in SoftWoRx 6.5.1.
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