The largest database of trusted experimental protocols

Taqman snp technology

Manufactured by Thermo Fisher Scientific
Sourced in United States

TaqMan SNP technology is a real-time PCR-based method used for the detection and analysis of single nucleotide polymorphisms (SNPs) in DNA samples. It utilizes sequence-specific oligonucleotide probes labeled with fluorescent dyes to enable the sensitive and quantitative measurement of target DNA sequences.

Automatically generated - may contain errors

9 protocols using taqman snp technology

1

Genotyping of PROX1 SNP

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA was extracted from the peripheral blood leukocytes using a classical salting out method. All SNPs were genotyped by TaqMan SNP technology from ready to use human assays library (Applied Biosystems, USA) using a high throughput genotyping system—OpenArray from Life Technologies (USA). SNPs analysis was performed in duplicate, following the manufacturer’s instructions. As a negative control, we used a sample without template. The negative control was helpful in measuring any false positive signal caused by contamination. No significant deviation from Hardy–Weinberg equilibrium was observed for the studied rs340874 SNP in PROX1 (p > 0.05).
+ Open protocol
+ Expand
2

Genotyping of FTO Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
We selected and genotyped 6 previously identified FTO SNPs in rs3751812 (G > T), rs8050136 (A > C), rs9939609 (T > A), rs6499640 (G > A), rs8044769 (C > T), and rs7190492 (A > G), based on the validated catalog of published genome-wide association studies [16 (link)]. DNA was extracted from peripheral blood leukocytes using a classical salting-out method. The SNPs were genotyped with TaqMan SNP technology from a ready-to-use human assay library (Applied Biosystems, Beverly, MA, USA) using a high-throughput genotyping system, OpenArray (Life Technologies, South San Francisco, CA, USA). SNP analysis was performed in duplicate, following the manufacturer’s instructions. As a negative control, we used a sample without a template. The negative control was applicable in measuring any false positive signal caused by contamination.
+ Open protocol
+ Expand
3

Genotyping of Blood DNA SNPs

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA was extracted from the peripheral blood leukocytes using a classical salting out method. All SNPs were genotyped by TaqMan SNP technology from ready to use human assays library (Applied Biosystems, USA) using a high throughput genotyping system—OpenArray from Life Technologies (USA). SNPs analysis was performed in duplicate following the manufacturer’s instructions. As a negative control, we used a sample without template. The negative control was helpful for measuring any false positive signal caused by contamination. No significant deviation from Hardy Weinberg equilibrium was observed for either of the SNPs in this study (all p > 0.05).
+ Open protocol
+ Expand
4

DNA Extraction and SNP Genotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA was extracted from the peripheral blood leukocytes using a classical salting out method. The SNPs were genotyped with TaqMan SNP technology from a ready-to-use human assay library (Applied Biosystems, MA, USA) using a high-throughput genotyping system, OpenArray (Life Technologies, CA, USA). A sample without a template was used as a negative control. The negative control was applicable in measuring any false positive signals caused by contamination.
+ Open protocol
+ Expand
5

Genotyping of Common FTO SNPs

Check if the same lab product or an alternative is used in the 5 most similar protocols
We genotyped 4 common FTO SNPs in rs3751812 (G > T), rs8044769 (C > T), rs8050136 (A > C), and rs9939609 (T > A). DNA was extracted from peripheral blood leukocytes using a classical salting-out method. The SNPs were genotyped with TaqMan SNP technology from a ready-to-use human assay library (Applied Biosystems, MA, USA) using a high-throughput genotyping system, OpenArray (Life Technologies, CA, USA). SNP analysis was performed in duplicate, following the manufacturer’s instructions. We used a sample without template as a negative control to detect possible false positive signals caused by contamination.
+ Open protocol
+ Expand
6

MC4R SNP Genotyping from Blood Leukocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
We genotyped previously identified MC4R SNPs in: rs17782313, rs12970134, rs633265, and rs1350341. DNA was extracted from peripheral blood leukocytes using a classical salting out method. The SNPs were genotyped with TaqMan SNP technology from ready-to-use human assays library (Applied Biosystems, Foster City, CA, USA) using a high throughput genotyping system (OpenArray, Life Technologies, Waltham, MA, USA). SNP analysis was performed in duplicate according to the manufacturer’s instructions. To detect possible false positive signals caused by contamination, a negative control consisting of a sample without a template was used.
+ Open protocol
+ Expand
7

Genotyping of TCF7L2 rs7901695

Check if the same lab product or an alternative is used in the 5 most similar protocols
We genotyped for the TCF7L2 SNP in rs7901695. The DNA was extracted from peripheral blood leukocytes, by a salting-out method described previously in detail [19 (link)]. Genotyping for the SNP was conducted using TaqMan SNP technology with a ready-to-use human assay library (Applied Biosystems, Waltham, MA, USA), using an OpenArray System (Life Technologies, Grand Island, NY, USA) as a tool for high-throughput genotyping. The genetic analyses were performed in duplicate in accordance with the manufacturer’s instructions. To detect possible false-positive signals caused by contamination, a negative control consisting of a sample without a template was used.
+ Open protocol
+ Expand
8

Genotyping BDNF Variants for Obesity Risk

Check if the same lab product or an alternative is used in the 5 most similar protocols
Based on the available scientific literature26 (link), we selected and genotyped 4 BDNF polymorphisms that are considered genetic risk factors for obesity: rs6265, rs4923461, rs10501087, and rs10835211. DNA was extracted from the peripheral blood leukocytes by a classical salting-out method. The analyzed SNPs were genotyped with a TaqMan SNP technology from a ready-to-use human assay library (Applied Biosystems, MA, USA) using a high-throughput system (Open Array, Life Technologies, CA, USA). We performed SNP analysis in duplicate, according to the manufacturer’s instructions. As a negative control we used a sample without a template to detect possibly caused by contamination false-positive signals.
+ Open protocol
+ Expand
9

FOXP3 Gene SNP Genotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA was extracted from the peripheral whole blood leukocytes using two extraction methods, the salting-out and the membrane method column separation (QIAamp DNA Blood Mini Kit, Qiagen, Germany). Both were performed in agreement to the manufacturer’s protocols. The purity of DNA was determined based on evaluation of the optical density ratio 260/280 nm.
The single nucleotide polymorphism analysis with was performed using the 7900HT Fast Real-Time PCR System (Applied Biosystems, USA). All SNPs in FOXP3 gene (rs3761549, rs3761548, rs3761547) were genotyped by fluorogenic TaqMan SNP technology from ready to use assays library (Applied Biosystems, Foster City, CA, USA) with the TaqMan Genotyping Master Mix (Applied Biosystems, Foster City, CA, USA) in 20 µl reaction volume. The final concentration of genomic DNA for all samples in the experiment sample was 10 ng/µl. The reactions were carried out under the following conditions: 10 min at 95 °C for starting polymerase activity, 40 cycles of 92 °C for 15 s and 60 °C for 1 min. SNPs analysis was performed in duplicate. As a negative control, a sample without DNA was used. The negative control was used to measure any false positive signal caused by contamination.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!