Taqman snp technology
TaqMan SNP technology is a real-time PCR-based method used for the detection and analysis of single nucleotide polymorphisms (SNPs) in DNA samples. It utilizes sequence-specific oligonucleotide probes labeled with fluorescent dyes to enable the sensitive and quantitative measurement of target DNA sequences.
Lab products found in correlation
9 protocols using taqman snp technology
Genotyping of PROX1 SNP
Genotyping of FTO Variants
Genotyping of Blood DNA SNPs
DNA Extraction and SNP Genotyping
Genotyping of Common FTO SNPs
MC4R SNP Genotyping from Blood Leukocytes
Genotyping of TCF7L2 rs7901695
Genotyping BDNF Variants for Obesity Risk
FOXP3 Gene SNP Genotyping
The single nucleotide polymorphism analysis with was performed using the 7900HT Fast Real-Time PCR System (Applied Biosystems, USA). All SNPs in FOXP3 gene (rs3761549, rs3761548, rs3761547) were genotyped by fluorogenic TaqMan SNP technology from ready to use assays library (Applied Biosystems, Foster City, CA, USA) with the TaqMan Genotyping Master Mix (Applied Biosystems, Foster City, CA, USA) in 20 µl reaction volume. The final concentration of genomic DNA for all samples in the experiment sample was 10 ng/µl. The reactions were carried out under the following conditions: 10 min at 95 °C for starting polymerase activity, 40 cycles of 92 °C for 15 s and 60 °C for 1 min. SNPs analysis was performed in duplicate. As a negative control, a sample without DNA was used. The negative control was used to measure any false positive signal caused by contamination.
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