Anti nedd4 rabbit polyclonal antibody

Manufactured by Merck Group

The Anti-NEDD4 rabbit polyclonal antibody is a laboratory reagent designed for the detection and analysis of the NEDD4 protein. NEDD4 is an E3 ubiquitin-protein ligase that plays a role in the regulation of various cellular processes. This antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of NEDD4 in biological samples.

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2 protocols using anti nedd4 rabbit polyclonal antibody

Western Blot and Immunohistochemistry Antibodies

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Antibodies used for Western blot assay were as follows: anti-AR rabbit polyclonal antibody (N-20, Cat#sc-816, Santa Cruz Biotechnology, Santa Cruz, CA); anti-PMEPA1 mouse monoclonal antibody (2A12, Cat#H00056937-M01, ABNOVA, Taiwan); anti-NEDD4 rabbit polyclonal antibody (Cat#07–049, Millipore, Billerica, MA); anti-PTEN mouse monoclonal antibody (Cat#MS-1601-S0, Lab Vision, Fremont, CA), anti-PSA rabbit polyclonal antibody (Cat#A05662, Dako, Denmark); anti-GAPDH polyclonal antibody (FL335, Cat#sc-25778, Santa Cruz Biotechnology, Santa Cruz, CA); and horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse TrueBlot antibodies (Cat#18–8816, Cat#18–8817-33, eBioscience, San Diego, CA). Antibodies for immunohistochemistry (IHC) assay were: anti-PMEPA1 antibody mentioned above, anti-AR mouse monoclonal antibody (M3562, DAKO, Denmark), anti-PSA rabbit polyclonal antibody (Cat#A0562, DAKO, Denmark), biotinylated horse anti-mouse antibody (Cat# BA-2000, Vector, Burlingame, CA), biotinylated goat anti-rabbit antibody (Cat# BA-1000, Vector, Burlingame, CA) and VIP peroxidase (HRP) substrate kit (Cat# SK-4600, Vector, Burlingame, CA).

Li H., Mohamed A.A., Sharad S., Umeda E., Song Y., Young D., Petrovics G., McLeod D.G., Sesterhenn I.A., Sreenath T., Dobi A, & Srivastava S. (2015). Silencing of PMEPA1 accelerates the growth of prostate cancer cells through AR, NEDD4 and PTEN. Oncotarget, 6(17), 15137-15149.

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Adipogenesis Regulatory Protein Assay

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Chloroquine, MG132, cycloheximide, rosiglitazone, GW 9662, isobutylmethylxanthine (IBMX), dexamethasone, insulin and oil red O were purchased from Sigma-Aldrich (St Louis, MO). The anti-NEDD4 rabbit polyclonal antibody (catalog# 07-049) was purchased from Millipore (Billerica, MA). The anti-C/EBPα rabbit polyclonal antibody (catalog# 2295) was obtained from Cell Signaling Technology (Danvers, MA), and the anti-PPARγ (E-8) mouse monoclonal antibody and the anti-PPARγ (H-100) rabbit polyclonal antibody were purchased from Santa Cruz Biotechnology (Dallas, TX). The anti-PPARγ rabbit monoclonal antibody (catalog# MA5-15003) was from Thermo Fisher Scientific (Waltham, MA). The anti-HA.11 epitope tag mouse monoclonal antibody (previously Covance catalog# MMS-101P) was purchased from BioLegend (San Diego, CA). The anti-FLAG epitope tag (M2, catalog# F1804), the anti-GAPDH (catalog# G8795) and the anti-β-actin (catalog# A2228) mouse monoclonal antibodies were from Sigma-Aldrich (St Louis, MO). The mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) was purchased from Enzo Life Sciences (Farmingdale, NY). The recombinant human PPARγ2 with His and GST tag (catalog# 501331) was purchased from NovoPro (Shanghai, China). The anti-Ub antibody was purchased from BD Biosciences (San Jose, CA).

Li J.J., Wang R., Lama R., Wang X., Floyd Z.E., Park E.A, & Liao F.F. (2016). Ubiquitin Ligase NEDD4 Regulates PPARγ Stability and Adipocyte Differentiation in 3T3-L1 Cells. Scientific Reports, 6, 38550.

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