ASCs were purchased from Invitrogen (Carlsbad, CA, USA) and cultured using MesenPRO RS Medium with supplied supplements (Invitrogen). ASCs were fed every 3–4 days with fresh media and were subcultured with 0.05% trypsin-EDTA (Gibco Life Technologies, Grand Island, NY, USA). For pioASCs, the ASC culture medium was treated with 3 μmol l−1 pioglitazone for 1 week. MLE-12 airway-epithelial cells were purchased from ATCC (Manassas, VA, USA), cultured with the recommended complete growth medium and subcultured with 0.05% trypsin-EDTA (Gibco Life Technologies).
Mle 12 airway epithelial cells
Manufactured by American Type Culture Collection
Sourced in United States
The MLE-12 airway-epithelial cells are a well-characterized cell line derived from mouse lung. These cells are commonly used as an in vitro model for studying the biology and function of airway epithelial cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mle 12 airway epithelial cells
1
Culturing Adipose-Derived Stem Cells
2
Nanovesicle Production from Cultured ASCs
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ASCs were purchased from Invitrogen (Carlsbad, CA, USA) and cultured using MesenPRO RS medium with supplied supplements (Invitrogen). ASCs were re-fed every 3–4 days with fresh media and were subcultured with 0.05% trypsin-EDTA (Gibco Life Technologies, Grand Island, NY, USA). Artificial nanovesicles were produced on passages 4–5. MLE-12 airway epithelial cells were purchased from the ATCC (Manassas, VA, USA), cultured with the recommended complete growth medium, and subcultured with 0.05% trypsin-EDTA (Gibco Life Technologies).
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