Lsm image browser software

Manufactured by Adobe

Sourced in United States

The LSM Image Browser software is a tool designed for the viewing and management of microscopy image data. It provides users with the ability to open, navigate, and explore images captured using laser scanning microscopes (LSM). The software offers basic functionalities for image visualization and file handling, without making interpretations or assumptions about the intended use of the product.

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Lab products found in correlation

Tcs sp5, by Leica (1 mentions) Lsm 510 meta, by Leica (1 mentions) E600 microscope, by Nikon (1 mentions) Neutral buffered formalin, by Merck Group (1 mentions) Apoptag red in situ apoptosis detection kit, by Merck Group (1 mentions) Prolong gold, by Thermo Fisher Scientific (1 mentions) Lsm 700 confocal microscope, by Zeiss (1 mentions)

Close spelling variants of this product

LSM Image Browser (10 citations) LSM 5 Image Browser software (4 citations) LSM software (3 citations)

3 protocols using lsm image browser software

Quantifying Melanopsin Dendritic Complexity

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Epifluorescence images were acquired on a Nikon E600 microscope equipped with a SPOT-RT Slider digital microscope camera. For wholemounts, we collected z-stacks of confocal images separated in depth by 0.5 or 1.0 µm on a Zeiss LSM510 Meta or Leica TCS SP5 confocal laser scanning microscope. Confocal images were analyzed using Zeiss LSM Image Browser software and further processed with Adobe Photoshop and ImageJ software.
To assess the density of outer retinal dendrites, we selected 3–5 regions of interest (680 µm × 450 µm) in wholemount retinas from each age studied (P4, P8, P12 and adult). In each of these regions, we counted the number of outer retinal dendrites (ORDs), defined as melanopsin immunopositive dendrites that extended outward into the inner plexiform layer. These dendrites were subdivided into simple and complex varieties based on whether, after entering the INL, they terminated within it (simple) or extended into and coursed within the OPL (complex).

Renna J.M., Chellappa D.K., Ross C.L., Stabio M.E, & Berson D.M. (2015). Melanopsin ganglion cells extend dendrites into the outer retina during early postnatal development. Developmental neurobiology, 75(9), 935-946.

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Detecting Apoptosis in Pelvic Ganglia

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After 4 days of culture, pelvic ganglia explants were fixed for 1 h at 4 °C with neutral buffered formalin (Sigma) and subsequently washed into 1 × PBS. Apoptotic cells were detected in intact explants with the ApopTag^® Red In Situ Apoptosis Detection Kit (EMD Millipore, S7165, Burlington, MA, USA) while still embedded in the collagen gel. Following this assay, the explants were subjected to immunochemistry with anti-Hu C/D antibody (a pan-neuronal marker, gift of V. Lennon, 1:10,000) overnight at 4 °C and a donkey anti-human Cy5 secondary at a 1:250 dilution for 1 h at room temperature. Explants were incubated for 5 min in 0.5 mg/mL DAPI and then transferred to glass slides and coverslipped using glycerin jelly (7.5% w/v glycerine, 55% v/v glycerol) and maintained at 4 °C in a dark, humidified chamber until imaging. Confocal microscopy was performed on a Zeiss Scanning Microscope LSM510 using a 633 nm laser for imaging Cy5 (649–745 band pass filter), 543 nm laser for imaging Cy3 (560–615 band pass filter), and a 488 nm laser for imaging Alexa 488 (505–550 band pass filter) to visualize the transgene expression and secondary antibody fluorophores. Images were captured with the Zeiss LSM Image Browser Software and then exported from the Image Browser software as.tiff files and assembled in Adobe Photoshop (2014 2.2 release, Adobe Systems Inc., San Jose, CA, USA).

Ritter K.E., Buehler D.P., Asher S.B., Deal K.K., Zhao S., Guo Y, & Southard-Smith E.M. (2021). 5-HT3 Signaling Alters Development of Sacral Neural Crest Derivatives That Innervate the Lower Urinary Tract. International Journal of Molecular Sciences, 22(13), 6838.

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Immunofluorescence Staining of HBMECs

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HBMECs were seeded onto fibronectin-coated coverslips (ca. 1 × 10 5 cells) and grown overnight to reach a confluency of 70-80%. Cells were infected for 4 h (MOI 200). Coverslips were washed with PBS and fixed with 4% paraformaldehyde (w/v) in PBS for 5 min. Coverslips were then washed with PBS and blocked in 4% (w/v) BSA/PBS at 4°C overnight. For intracellular staining, cells were permeabilised using 0.1% Saponin, 20 mM glycine in 4% BSA/TBS at 4°C. Subsequent staining procedures were carried out in 4% (w/v) BSA/TBS. Briefly, coverslips incubated with primary antibody for 1 h were washed with PBS followed by one wash with dH2O. Coverslips were then incubated with secondary antibody for 1 h in the dark followed by washes with PBS-Tween (0.05% v/v; PBS-T), PBS and then dH2O. Coverslips were then mounted on glass slides with ProLong® Gold and SlowFade® Gold Antifade Reagents with DAPI (Invitrogen). Coverslips were analysed using a Zeiss LSM-700 confocal microscope. Images were processed with ImageJ, Adobe Photoshop and LSM Image Browser software.

Azimi S., Wheldon L.M., Oldfield N.J., Ala'Aldeen D.A.A, & Wooldridge K.G. (2020). A role for fibroblast growth factor receptor 1 in the pathogenesis of Neisseria meningitidis. Microbial pathogenesis, 149.

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