The largest database of trusted experimental protocols

4 protocols using wortmannin

1

Nanoparticle Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wortmannin and KU60019 were purchased from Apex Bio (Houston Texas). Methoxypoly(ethylene glycol)-block-poly(lactic-co-glycolic acid) (mPEG-PLGA) with molecular weights of 2000:15000 (PEG(2K):PLGA(15K)) and 5000:1000 Da (PEG(5K):PLGA(10K)) were purchased from Akina (PolySciTech, West Lafayette, IN). Rhodamine B-labeled PLGA with approximate molecular weight 30000 was purchased from Akina Inc. (PolySciTech, West Lafayette, IN). Poly(D,L-lactide) (PLA), ester terminated with average MW 18000-28000 was purchased from Sigma-Aldrich (St. Louis, MO). Acetonitrile (HPLC grade) and double distilled water (HPLC grade) were obtained from Sigma-Aldrich (St. Louis, MO).
+ Open protocol
+ Expand
2

Investigating SDF1α-Mediated Cell Migration

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human SDF1α was purchased from PeproTech; UK14304, rapamycin, brefeldin A, monensin, nigericin, swainsonine, and Exo2 were from Sigma–Aldrich; pertussis toxin was from List Biological Laboratories; gallein was from Tocris Bioscience; wortmannin, AS-604850, and GSK2292767 were from ApexBio; TGX-221 and HS-173 were from Adooq Bioscience; secinH3, GCA, nocodazole, ilimaquinone, AMD3100, control siRNA, and siRNA targeting human ARF1, CRISPR–Cas9 control plasmids, and knockout plasmids targeting human ARF1, and antibodies against phospho-ERK1/2 and β-actin were from Santa Cruz Biotechnology; p230 antibodies were from BD Transduction Laboratories; ARF1 antibodies were purchased from Abcam; ERK1/2 antibodies were from Cell Signaling Technology; 12-well inserts and Matrigel matrix were from Corning. All other materials were obtained as described elsewhere (9 (link), 19 (link), 27 (link)).
+ Open protocol
+ Expand
3

Investigating PI3Kγ Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human SDF1α was purchased from PeproTech; UK14304, GW5074, rapamycin, BFA, ilimaquinone, monensin, nigericin, swainsonine, and LY294002 were from Sigma Aldrich; nocodazole, insulin-like growth factor 1, AMD3100, control siRNA (medium GC), siRNAs targeting to human PI3Kγ regulatory subunits p101 and p87, and antibodies against GFP, phospho-ERK1/2, Gγ9, and β-actin and the PI3Kγ subunits p110γ, p101 and p87 were from Santa Cruz Biotechnology; wortmannin, AS-604850, and GSK2292767 were from ApexBio; TGX-221 and HS-173 were from Adooq Bioscience; U0126 and PD98059 were from Calbiochem; EGF, puromycin, and blasticidin S were from Thermo Fisher Scientific; U-73122 and AZD5363 were from MedChemExpress; U-73433, CRT0066101, and Go6976 were from Cayman Chemical; PTX was from List Biological Laboratories; gallein and rauwolscine were from Tocris Bioscience; D-Luciferin was from GoldBio; antibodies against hemagglutinin and ERK1/2 were from Cell Signaling Technology; antibodies against Gγ3 were from Abcam. All other materials were obtained as described (52 (link), 53 (link)).
+ Open protocol
+ Expand
4

Pharmacological Inhibition of Viral Endocytosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The pharmacological inhibitors’ concentration used in our study is based on previous research (Table 1) [21 (link), 22 (link)]. Inhibitors were prepared as follows: pistop2, dynasore, rottlerin, nystatin, wortmannin, Bafilomycin A1, Latrunculin B, nocodazole, IPA-3, and amiloride were purchased from ApexBio (Houston, USA). Chlorpromazine (CPZ) was purchased from Selleck. Stock solutions of inhibitors were dissolved in dimethyl sulfoxide (DMSO), except Latrunculin B which was dissolved in ethanol according to the manufacturer’s instruction. Ammonium chloride (NH4Cl) and methyl-β-cyclodextrin were bought from Sigma and dissolved in H2O.

Variables from the questionnaire of the HBSC study analyzed in this study

InhibitorTargetHighest concentration
Pistop2CME5 μM (GCRV 104); 25 μM (GCRV JX01)
CPZCME10 μM (GCRV 104; GCRV JX01)
NystatinCaveola-dependent endocytosis15 μM (GCRV 104)
methyl-β-cyclodextrinCaveola-dependent endocytosis1 mM (GCRV 104)
dynasoreDynamin10 μM (GCRV 104)
wortmanninPI3K5 μM (GCRV 104)
IPA-3Pak120 μM (GCRV 104)
rottlerinPKC10 μM (GCRV 104)
NH4ClPH20 mM (GCRV 104)
AmilorideNHE10 μM (GCRV 104)
Bafilomycin A1Vacuolar ATPas inhibition2 nM (GCRV 104)
nocodazoleMicrotubules10 μM (GCRV 104)
Latrunculin BActin microfilaments0.5 μM (GCRV 104)
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!