Cell culture supplements

All cell culture serum and media were purchased from Thermo Fisher Scientific, while cell culture supplements were purchased from Sigma. Rat anti-CD31 (553369) was purchased from Pharmingen while antibodies against alpha SMA (ab7817), phosphor-ErK (ab50011) and ErK1/2 (ab17942) were from Abcam. The antibodies against phospho-Akt (sc-7985R), Akt1 (sc-1619) and GAPDH (sc-25778) were from Santa CruZ Biotech. The antibody against VEGF (SAB1402390) was from Sigma. All secondary antibodies were from Dakocytomation. All microRNA reagents were purchased from Thermo Fisher Scientific. PDGF, insulin, holo-transferrin, SU5416, LY294002, DMSO, DAPI and Giemsa were purchased from Sigma.

DMEM medium and cell culture supplements were purchased from Sigma (Steinheim, Germany). Cell culture plastics, phosphate-buffered saline (PBS), and fetal calf serum (FCS) were purchased from PAA Laboratories GmbH (Pasching, Austria). DNase I (RNase-free) and First Strand cDNA Synthesis Kit were purchased from Fermentas (Ontario, Canada). 5-AZA and L-ascorbic acid 2-phosphate were obtained from Sigma-Aldrich (Steinheim, Germany). All other chemical compounds were purchased from Carl Roth (Karlsruhe, Germany). 5-hmC (39769) mouse mAB was purchased from Active Motif (Carlsbad, CA, USA). Proliferating cell nuclear antigen (PCNA) (ab92552) rabbit mAB was obtained from Abcam (Cambridge, UK). Corresponding secondary antibodies goat anti-rabbit Alexa 555 and goat anti-mouse 488 were acquired from Invitrogen (Carlsbad, CA, USA). Anti TET2 (SAB3500711), anti TET3 (SAB2700682), and anti glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (G9545) antibodies were used from Sigma (Munich, Germany). GADD45B (SC33172) rabbit mAB was from Santa Cruz, and Snail (3879) rabbit mAB, E-cadherin (14472) mouse mAB, p21 (2947) rabbit mAB, cyclin-B1 (4138) rabbit mAB, and HRP-linked anti-mouse and anti-rabbit IgG secondary antibody were purchased from Cell Signaling (Beverly, MA, USA).