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30 protocols using quantum fx micro ct

1

Laser-assisted Ablation of Artificial Stones

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Stone phantoms were fully immerged with saline solution at ambient temperature and fixed into a bench model. The laser fiber tip was placed perpendicularly and in contact with the surface of the stone. A specific fiber support was manufactured in order to assure the permanent contact with the artificial stone during laser emission (Figure 1). First, a two seconds-firing was executed without displacement, in order to determine the static ablation rate. Then, a robotic six-axes arm (KR6R900, Kuka International©, Augsbourg, Germany) was used to realize multiple linear trajectories: 5 mm, 10 mm and 20 mm, maintaining a displacement duration of two seconds. The laser emission and the robotic arm were initiated and stopped jointly by computational command. All experiments were repeated four times. After laser emission, stones were dried as described above.
To assess the ablation volumes, a three-dimensional scanning (micro-CT Quantum FX, Perkin Elmer©, Waltham, MA, USA) of the artificial stones and subsequent 3D segmentation using 3DSlicer software (NIH©) was used (Figure 2) [4 (link),14 (link),15 (link)].
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2

Laser-Assisted In Vitro Kidney Stone Ablation

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Immerged stones were immobilized into a bench model and filled with saline solution (NaCl 0.9%) at ambient temperature. No irrigation was required for our study. The laser fiber was placed vertically in contact with the sample, controlled by a micrometric screw. A specific fiber support was manufactured to assure perfect immobility during laser emission (Fig. 1). The laser machine was modified to deliver a single pulse emission. After each emitted pulse, we cleaved the laser fiber with ceramic scissors. Figure 1 shows the experimental conditions for in vitro tests. All laser pulses were repeated three times per stone sample. After lithotripsy, stones were dried as previously described and scanned (micro-CT Quantum FX, Perkin Elmer © ) at the Life Imaging Platform (Paris Descartes University, Montrouge, France) with a visual range of 20 mm and a 10 µm resolution. Ablation volumes per pulse (mm 3 ) were assessed by 3D segmentation using 3D Slicer (NIH © ) (Fig. 2) [11] . The required energy (RE) to ablate 1 mm 3 was then calculated from PE and ablation volumes.
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3

Small Animal Micro-CT and Bioluminescence Imaging

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Mice were scanned for 340 s under isoflurane anesthesia using a small animal Quantum FX Micro-CT (PerkinElmer) at 45-mm resolution and 90 kV with a 160-mA current. Images were acquired and analyzed with PerkinElmer Quantum FX software. For bioluminescent imaging, mice were given 150 mg kg−1 d-luciferin potassium salt (Biovision, 7903–10PK) intraperitoneally and imaged on the IVIS Spectrum In Vivo Imaging system (PerkinElmer).
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4

Small Animal Micro-CT Imaging Protocol

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At indicated time points, mice were scanned for 30 s to 2 min under isoflurane anesthesia using a small animal Quantum FX microCT (PerkinElmer) at 45 υm resolution, 90 kV, with 160 υA current. Images were acquired using PerkinElmer Quantum FX software and processed with Analyze 11.0 (AnalyzeDirect).
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5

Small Animal Micro-CT Imaging Protocol

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At indicated time points, mice were scanned for 30 s to 2 min under isoflurane anesthesia using a small animal Quantum FX microCT (PerkinElmer) at 45 υm resolution, 90 kV, with 160 υA current. Images were acquired using PerkinElmer Quantum FX software and processed with Analyze 11.0 (AnalyzeDirect).
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6

Micro-CT Analysis of Meniscus

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Micro-CT measurement was taken for 3 min using a Quantum FX Micro-CT (Perkin Elmer, Waltham, MA, USA) set at a tube voltage of 90 kVp, tube current of 160 μA, and FOV of 10 mm, and image analysis was performed using Analyze 12 (Mayo Clinic, Scottsdale, AZ, USA). In this analysis, volume rendering was performed after the experiment was completed to separate the meniscus, and then a 3D image was obtained.
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7

Micro-CT Scaffold Characterization

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The scaffold samples were harvested after intervention and fixed in 4% paraformaldehyde. Subsequently, the samples were scanned by micro-CT (Quantum FX microCT, PerkinElmer, USA); RigakuTM software (Rigaku, Japan) and RadiAnt DICOM ViewerTM (Medixant, Poland) were used for 3D reconstruction and image processing.
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8

μCT Imaging with Quantum FX microCT

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μCT images were obtained with a Quantum FX microCT (Perkin Elmer) as described previously [54 (link)].
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9

Micro-CT Analysis of Replanted Tooth Bone Resorption

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The extracted maxilla was scanned at 10 µm intervals using a micro-computed tomography (micro-CT) system (Quantum FX micro-CT, Perkin Elmer, Norwalk, CT, USA), and the tooth images were reconstructed using a software (TRI-3D, Ratoc System Engineering Co., Ltd., Tokyo, Japan). The mesiobuccal (MB) root was chosen as it is the largest and allows clear observation of changes. A longitudinal image of the replanted tooth was obtained through the long axis of the MB root in the mesiodistal direction. The scoring standards are listed in Table 2. The extension of the bone surface resorption area in contact with the root was measured in micrometers. Then, the ratio of the total surface of the bone in contact with the root was calculated.
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10

Micro-CT Analysis of Alveolar Bone Density

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Micro CT (Quantum FX micro-CT, Perkin Elmer, Waltham, MA, USA) were scanned at tube voltage (90 kVp), tube current (160 μA), imaging time (180 sec), FOV (field of view, 5 mm), and Pixil size (10 μM). To measure bone mineral density (BMD), the direction was changed to a coronal section, and the raw data obtained from Micro-CT were loaded into CTAn and scanned images, bone mineral density (BMD) were obtained based on the above threshold. The region of interest (ROI) was set using the interpolation method of the alveolar bone except the root of the cement–enamel junction to the root. Data were expressed as mean ± SD. The significance of the data was analyzed by one-way ANOVA using SPSS Statistics (Armonk, NY, USA).
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