Nine 8-week-old C57BL/6 mice were randomly divided into three groups (n = 3), and then the mice were anesthetized with isoflurane. After that, IR780 (Merck, Germany) labeled PTH (1–34) PBS solution, GGA pre-hydrogel solution, and IR780 labeled Lipo@PTH (1–34) in GGA pre-hydrogel solution to make IR780 labeled GLP pre-hydrogel solution were prepared and each kind solution was intra-articularly injected in a mice. At the set time point, the mice were imaged by means of an IVIS at the excitation wavelength of 657 nm and emission wavelength of 747 nm to determine the retention of injected materials.
Ir780
Manufactured by Merck Group
Sourced in United States, Germany
IR780 is a near-infrared dye compound that can be used in various analytical and research applications. It exhibits strong absorption and emission in the near-infrared region of the electromagnetic spectrum. The core function of IR780 is to serve as a fluorescent label or marker for detection and imaging purposes.
Automatically generated - may contain errors
Lab products found in correlation
Cell counting kit 8 cck 8, by Dojindo Laboratories (5 mentions)
Coumarin 6, by Merck Group (3 mentions)
Dspe mpeg2000, by Xi’an Ruixi (3 mentions)
1 2 distearoyl sn glycero 3 phosphoethanolamine n methoxy polyethylene glycol 2000 dspe mpeg2000, by Xi’an Ruixi (3 mentions)
Pluronic f 127, by Merck Group (2 mentions)
Curcumin, by Merck Group (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Ir 808, by Merck Group (2 mentions)
Ir783, by Merck Group (2 mentions)
Dulbecco s modified eagle s medium dmem, by Cytiva (2 mentions)
Singlet oxygen sensor green (sosg), by Thermo Fisher Scientific (2 mentions)
H2dcfda, by Merck Group (1 mentions)
Ufc910024, by Merck Group (1 mentions)
Chlorpromazine, by Merck Group (1 mentions)
C2920, by Merck Group (1 mentions)
Methyl β cyclodextrin mβcd, by Merck Group (1 mentions)
Oligomycin a, by Merck Group (1 mentions)
Rotenone, by Merck Group (1 mentions)
Human serum albumin (hsa), by Merck Group (1 mentions)
Accuri c6 flow cytometer, by BD (1 mentions)
41 protocols using ir780
1
IR780 Labeled PTH and GLP Retention
2
Efficient Dye-Loaded Polymeric Beads for Raman Imaging
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Rdots were prepared based on previously published procedures (23 (link), 24 (link), 25 (link)). Here, IR780 (425,311, Sigma Aldrich) was used instead of IR783 (Fig. 1a ) because IR780 is incorporated into beads more efficiently than IR783, while their Raman spectra are the same. A mixture of 150-µL, 2.5%, 40-nm PS beads (Invitrogen, C37232), 150-µL, 2%, F-127 (Invitrogen, P6867), and 300-µL Milli-Q were put in a 1.5-mL plastic tube. The mixture of 30-µL, 30-mM cyanine dye solution in DMSO and 270-µL tetrahydrofuran was added to the tube dropwise. The mixture was then vortexed for 10 min, followed by gentle agitation for another 10 min. Then, 14-mL Milli-Q water was added to the mixture to quench the dye loading. The mixture was then centrifuged (4000 rpm, 10 min) using 100 K MWCO filters (Millipore, UFC910024) to remove the solvents. The stained beads were washed with 15-mL Milli-Q water five times.
3
Synergistic Nanotherapy for Prostate Cancer
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HSA solutions were purchased from Baxter. DTX, IR780, and dichlorodihydrofluorescein diacetate (H2DCFDA) were both purchased from Sigma Aldrich. The cell counting kit-8 (CCK-8) was supplied by Dojindo Laboratories (Tokyo, Japan). The obtained HSA@IR780@DTX NPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), and ultraviolet-visible (UV-Vis) spectrophotometry. It showed simultaneous synergistic PDT/PTT and chemotherapy treatment effect in vitro and in vivo for castration-resistant prostate cancer. Deionized water was used throughout the experiments. We purchased 22RV1 cell lines from the Institute of Biochemistry and Cell Biology, Shanghai Institute of Life Sciences, Chinese Academy of Sciences. All the nude mice were purchased from Yangzhou University Medical Center, and the weight of each mouse was between 20 and 22 g.
4
Cellular Uptake of IR-780 Dye
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H9C2 cells were seeded into six‐well plates at a density of 5 × 105 cells and were used for further experiments when up to 90% of confluence was reached. The cells were treated with DMEM with amiloride (75 µg mL−1, HY‐B0285, MCE), BSP (250 µm , S0252, Sigma), chlorpromazine (15 µg mL−1, 31 679, Sigma), methyl‐β‐cyclodextrin (MβCD, 7.5 mm , 779 776, Sigma), FCCP (10 µm , C2920, Sigma), rotenone (10 µm , 45 656, Sigma), oligomycin A (10 µm , 495 455, Sigma), HSA (1 µm , A1887, Sigma), HSA (1 µm , A1887, Sigma) + ibuprofen (100 µm , I0415, TCI), or ice or hypoxic conditions (5% O2) or different pH conditions (5.2, 6.2, 7.4, and 8.9) for 30 min; subsequently, IR‐780 (0.1 µm , 425 311, Sigma) was added into the medium for another 20 min. Next, the cells were washed thrice with PBS and were for further NIR imaging using the Leica NIR fluorescent microscope and flow cytometric analysis using a BD Accuri C6 Flow Cytometer.
5
Synthesis of Iron Oxide Nanoparticles
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All analytically pure reagents were used as received without further purification. Iron (III) acetylacetonate, ethyl acetate, oleic acid, polyvinyl alcohol (PVA), chloroform, ethanol, oleylamine, benzyl ether, and dimethyl sulfoxide (DMSO) were ordered from Sinopharm Chemical Reagent Co., Ltd; PLGA, 1,2-hexadecanediol and IR780 were purchased from Sigma-Aldrich.
6
Liposomal Doxorubicin and IR-780 Formulation
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1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholine (MPPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) were purchased from Avanti Polar Lipids Inc. (Alabaster, AL,USA). Doxorubicin and IR-780 were obtained from Sigma-Aldrich (St. Louis, MO, USA). The in situ Cell Death Detection Kit and DAPI were obtained from Roche (Mannheim, Germany). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Tokyo, Japan). Fetal bovine serum (FBS), Trypsin EDTA solution and Dulbecco's Modified Eagle's Medium (DMEM) were all purchased from Hyclone Laboratories (Logan, UT, USA). Female BALB/c mice, weighing about 20 g (6-8 weeks old), were obtained from Guangdong Medical Experimental Animal Center (Guangzhou, China). All other reagents were of analytical grade.
7
Cardiolipin Profiling via Mass Spectrometry
8
Nanoparticle Synthesis and Characterization
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DTPA,
mPEG (Mn 2000), CPZ, GSH, C6, and IR-780
were purchased from Sigma-Aldrich (St. Louis, MO, USA). BMS202 was
obtained from MedChemExpress (Monmouth Junction, NJ, USA), while capsaicin
was purchased from Tokyo Chemical Industry (Tokyo, Japan). Acetyl
chloride, N,N′-dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine
(DMAP), and triethylamine (TEA) were acquired from Alfa Aesar (Ward
Hill, MA, USA). 4T1 cells were obtained from the American Type Culture
Collection (ATCC, Manassas, VA, USA). All other chemicals and reagents
were of analytical grade.
mPEG (Mn 2000), CPZ, GSH, C6, and IR-780
were purchased from Sigma-Aldrich (St. Louis, MO, USA). BMS202 was
obtained from MedChemExpress (Monmouth Junction, NJ, USA), while capsaicin
was purchased from Tokyo Chemical Industry (Tokyo, Japan). Acetyl
chloride, N,N′-dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine
(DMAP), and triethylamine (TEA) were acquired from Alfa Aesar (Ward
Hill, MA, USA). 4T1 cells were obtained from the American Type Culture
Collection (ATCC, Manassas, VA, USA). All other chemicals and reagents
were of analytical grade.
9
Multifunctional Nanoparticle-Mediated Tumor Therapy
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Doxorubicin and ε-Caprolactone were purchased from Aladdin co. (Shanghai, China). IR780 was purchased from Sigma-Aldrich co. (St. Louis, USA), PEG was obtained from Peng Sheng Biological Co., Ltd (Shanghai, China). Poly(lactic-co-glycolic acid) (PLGA) and human serum albumin was obtained from Aladdin co. (Shanghai, China). MTT Cell Proliferation and Cytotoxicity Assay Kit was obtained from Beyotime (Shanghai, China). TUNEL Apoptosis Assay Kit was provided by Beyotime Institute of Biotechnology (Haimen, China). Ki67 antibody was provided by Cell Signaling Technology (MA, USA). 1 × Phosphate buffer solution (PBS) and deionized water were used in the experiments. All C57BL/6 female mice (18–20 g) were obtained from Yangzhou University Medical Center. All other reagents were purchased from Nanjing Wanqing Chemical Glassware Instrument Company and used as received.
10
Multimodal Evaluation of Nanocarrier-Mediated Therapy
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IR-780, 2′,7′-dichloro-dihydrofluorescein diacetate (DCFH-DA) and cetyl trimethyl ammonium bromide (CTAB) were purchased from Sigma-Aldrich (St Louis, MO, USA). DOX, 4′,6′-diamidine-2′-phenylindole (DAPI) and singlet oxygen sensor green (SOSG) were bought from Meilun Biotech (Dalian, China). Sodium salicylate (NaSal) and tetraethyl orthosilicate (TEOS) were purchased from Heowns Biochemical Technology (Tianjin, China). Annexin-V-FITC/7AAD apoptosis kit was bought from (BD Biosciences, San Jose, USA). LIVE/DEAD cell imaging kit and monoclonal antibodies against CD11a-FITC and CD62P-APC were provided by eBioscience (San Diego, CA, USA). Coomassie brilliant blue methylthiazolyldiphenyl-tetrazolium bromide (MTT) and TUNEL apoptosis assay kit were sourced from SolarbioScience & Technology (Beijing, China). Rabbit anti-CD31 polyclonal antibody was purchased from Abcam (Cambridge, MA, USA).
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