Histological studies of the soft tissue samples (fibrous capsules) located above the cervical area of the dental implants were performed after the second surgical stage of dental implantation and biopsy sampling. The material was placed in a 10% solution of neutral formalin for 72 h, after which, the tissue samples were washed in running water for 2 h. After standard histological preparation, the tissue samples were embedded in paraffin (Histomix, Biovitrum, St. Petersburg, Russia), using histological pouring rings (Biovitrum, St. Petersburg, Russia). Serial and semi-serial slices were made from the blocks on a Microm microtome (3–7 μm). The specimens were stained by Mallory technique (Biovitrum, St. Petersburg, Russia) to reveal specific processes of connective tissue formation.
Mallory technique
Manufactured by BioVitrum
Sourced in Sao Tome and Principe
The Mallory technique is a staining method used in histology and pathology to visualize certain cellular and tissue structures. It is primarily used to stain collagen fibers, which appear blue or green in the final stained preparation. The technique involves the use of different dyes and dehydration steps to achieve the desired staining pattern. The Mallory technique provides a reliable way to identify and analyze collagen-rich structures within biological samples.
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Lab products found in correlation
2 protocols using mallory technique
1
Histological Analysis of Dental Implant Capsules
2
Histological Analysis of Peri-Implantitis Granulation Tissue
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To increase the reliability of the results of physical study methods, duplicate histological studies of soft tissue biopsy specimens of the bone bed granulation tissue were performed.
A histological study of the tissue samples was performed immediately after the biopsy was obtained as a result of surgical treatment of peri-implantitis. The material was placed in a 10% solution of neutral formalin for 72 h, after which the tissue samples were washed in running water for 2 h. After standard histological preparation, the tissue samples were embedded in paraffin (Histomix, Biovitrum, St. Petersburg, Russia) using histological pouring rings (Biovitrum, St. Petersburg, Russia). Serial and semi-serial slices were made from the obtained blocks on a Microm microtome (from 3 to 7 μm). To reveal specific processes of connective tissue formation, the preparations were stained using the Mallory technique (Biovitrum, St. Petersburg, Russia).
A histological study of the tissue samples was performed immediately after the biopsy was obtained as a result of surgical treatment of peri-implantitis. The material was placed in a 10% solution of neutral formalin for 72 h, after which the tissue samples were washed in running water for 2 h. After standard histological preparation, the tissue samples were embedded in paraffin (Histomix, Biovitrum, St. Petersburg, Russia) using histological pouring rings (Biovitrum, St. Petersburg, Russia). Serial and semi-serial slices were made from the obtained blocks on a Microm microtome (from 3 to 7 μm). To reveal specific processes of connective tissue formation, the preparations were stained using the Mallory technique (Biovitrum, St. Petersburg, Russia).
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