Typhoon rgb
The Typhoon RGB is a multipurpose fluorescence and phosphorescence imager designed for a wide range of applications in life science research. It offers high sensitivity and dynamic range for the detection and quantification of proteins, nucleic acids, and other biomolecules labeled with a variety of fluorescent dyes or radiolabels.
Lab products found in correlation
9 protocols using typhoon rgb
DGK-θ Kinase Activity Assay
Quantitative Northern Blot Analysis of gRNA
Pseudouridylation Detection Protocol
Quantifying Fungal Hydrogen Sulfide Production
Candida albicans and negative strains were cultured in the MTB medium; then,
Selective RNA Cleavage by cASO-CB Complex
Fungal Identification via Fluorescent Tagging
and coated on to a potato agar plate, which was cultured at 32 °C
for about 5 days until the development of obvious fungal colonies.
The potato agar plate contained penicillin (100 U/mL)/streptomycin
(0.1 mg/mL), to inhibit intestinal bacteria. Then,
°C for 2 h. The plates were then imaged using an Amersham Typhoon
RGB, and the fluorescence images recorded (λex =
635 nm, λem = 670 ± 15 nm). Then, the colonies
exhibiting fluorescence emission were purified and identified by the
intergenic internal transcribed spacer 1 (ITS1) region sequencing
using paired universal primers ITS1 (TCCGTAGGTGAACCTGCGG)/ITS2(GCTGCGTTCTTCATCGATGC),
respectively.
Fluorescent Stem-Loop Substrate Assay
High-Throughput Screening for PGP-1 Inhibitors
The herbs used are listed in Table S1. Then, the plate was subjected to fluorescence imaging (λex = 635 nm, λem = 670 ± 15 nm) using an Amersham Typhoon RGB.
According to the fluorescence intensity of individual wells, the inhibitory effect of each herb was determined. The inhibitory effect of isolated compounds was evaluated using the same protocol.
Isolation and Identification of Gut Bacteria
The fresh stools were dispersed in sterile water immediately, which was then cultured on nutrient broth agar medium (NB) until the formation of obvious colonies (37 ºC, 48 h). When the bacterial colonies were observed on the agar plates, DDPA was dropped onto the bacterial colonies for a co-incubation of 5 h. Then, fluorescence images of the agar plates were obtained using an Amersham Typhoon RGB (λex 635 nm, λem 670 ± 15 nm). For the bacterial colonies with strong fluorescence intensity, a further purification was performed on the NB culture. The isolated bacteria strains were identified by 16s rDNA sequence. The RT-PCR primers were as follows.
1510 R: 5'-ACGGYTACCTTGTTACGACTT-3' 7F: 5'-AGAGTTTGATYMTGGCTCAG-3'
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