Log In Sign up
The largest database of trusted experimental protocols

Cd20 l26

Manufactured by Biocare Medical
Visit Supplier

The CD20 (L26) is a laboratory antibody reagent used for the identification and enumeration of B-lymphocytes in biological samples. It detects the CD20 antigen, which is expressed on the surface of B-cells.

Automatically generated - may contain errors

Lab products found in correlation

Background sniper, by Biocare Medical (2 mentions) H550s microscope, by Nikon (2 mentions) Cd68 kp1, by Agilent Technologies (2 mentions) Diva decloaker, by Biocare Medical (2 mentions) Mach3 mouse hrp polymer detection, by Biocare Medical (2 mentions) Cd45 lca 2b11 pd7 26, by Agilent Technologies (2 mentions) Cd3 sp7, by Thermo Fisher Scientific (2 mentions)

2 protocols using cd20 l26

1

Immunohistochemical Analysis of Human Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues for IHC were harvested from MoM and fixed in 4% paraformaldehyde for 24 hour at 4°C, embedded in paraffin, cut into 5-μm sections and mounted onto poly-L-lysine coated glass slides. Following paraffin removal, antigen retrieval (DIVA Decloaker, Biocare Medical, Cat. DV2004.) and blocking of non-specific Ig-binding sites (Background Sniper, Biocare Medical), tissue sections were stained with primary antibodies overnight at 4°C and developed with a biotin-free horseradish peroxidase (HRP)-polymer system (MACH3 Mouse HRP-Polymer Detection, Biocare Medical). All tissue sections were then counterstained with hematoxylin. Primary antibodies directed against CD45 LCA (2B11&PD7/26, Dako), CD3 (SP7, Thermo Scientific), CD20 (L26, Biocare Medical) and CD68 (KP1, Dako) were used to identify human cells in the spleen, liver and lung. For comparison, tissue sections were stained with mouse IgG1k or IgG2a isotype controls. Light microscopy images were taken with a Nikon H550S microscope at 40×.
Honeycutt J.B., Thayer W.O., Baker C.E., Ribeiro R.M., Lada S.M., Cao Y., Cleary R.A., Hudgens M.G., Richman D.D, & Garcia J.V. (2017). HIV persistence in tissue macrophages of humanized myeloid only mice during antiretroviral therapy. Nature medicine, 23(5), 638-643.
+ Open protocol
+ Expand
2

Immunohistochemical Analysis of Human Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues for IHC were harvested from MoM and fixed in 4% paraformaldehyde for 24 hour at 4°C, embedded in paraffin, cut into 5-μm sections and mounted onto poly-L-lysine coated glass slides. Following paraffin removal, antigen retrieval (DIVA Decloaker, Biocare Medical, Cat. DV2004.) and blocking of non-specific Ig-binding sites (Background Sniper, Biocare Medical), tissue sections were stained with primary antibodies overnight at 4°C and developed with a biotin-free horseradish peroxidase (HRP)-polymer system (MACH3 Mouse HRP-Polymer Detection, Biocare Medical). All tissue sections were then counterstained with hematoxylin. Primary antibodies directed against CD45 LCA (2B11&PD7/26, Dako), CD3 (SP7, Thermo Scientific), CD20 (L26, Biocare Medical) and CD68 (KP1, Dako) were used to identify human cells in the spleen, liver and lung. For comparison, tissue sections were stained with mouse IgG1k or IgG2a isotype controls. Light microscopy images were taken with a Nikon H550S microscope at 40×.
Honeycutt J.B., Thayer W.O., Baker C.E., Ribeiro R.M., Lada S.M., Cao Y., Cleary R.A., Hudgens M.G., Richman D.D, & Garcia J.V. (2017). HIV persistence in tissue macrophages of humanized myeloid only mice during antiretroviral therapy. Nature medicine, 23(5), 638-643.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!