Percp5.5 cd14

Manufactured by BioLegend

Sourced in United States

The Percp5.5-CD14 is a fluorescent-labeled antibody that binds to the CD14 surface antigen. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein that is expressed on the surface of monocytes and macrophages. The Percp5.5 fluorescent label allows for the detection and analysis of CD14-positive cells using flow cytometry.

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Lab products found in correlation

Facsaria 3, by BD (1 mentions) Apc cd90 5e10, by BioLegend (1 mentions) Macsquant analyzer 10, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

PerCP5.5

2 protocols using percp5.5 cd14

Purification and Sorting of Synovial Fibroblasts

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Single-cell suspension of synovial tissue was purified into fibroblasts after 4 passages in one month. Single-cell suspension of SF was stained with FITC-TCRb and percp5.5-CD14 (Biolegend, USA) at dark 4 °C for 10–15 min. After washing and centrifugation, cell pellets were resuspended with propidium iodide/PBS buffer (Biolegend, USA) and sorted into T cells and monocytes by BD FACSAria™ III. Single-cell suspension of each subset was pooled from 6 OA patients.

Xiang C., Hong S.M., Zhao B., Pi H., Du F., Lu X., Tang Y., Shen N., Yang C, & Wang R. (2024). Fibroblast expression of neurotransmitter receptor HTR2A associates with inflammation in rheumatoid arthritis joint. Clinical and Experimental Medicine, 24(1), 84.

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Phagocytic Assay with E. coli Bioparticles

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Phagocytic assays were performed using the pHrodo Green E. coli Bioparticle conjugate system (cat# P35366, Invitrogen) according to manufacturer recommendations. Activation of fluorescence of the pHrodo Green in this system is pH dependent and is detected only when internalized within the phagosome of the cell. Green E. coli Bioparticle conjugate was reconstituted in PBS, diluted in media, and incubated with the cells for 1 hour at 37C. Cells were then washed with cold PBS three times to reduce non-specific attachment collected using a cell scraper. Collected macrophages were then treated with Fc Receptor blocker for 10 minutes and stained for 20 minutes at 4° C with PerCP 5.5-CD14 and with APC-CD90 (5E10, cat # 328113, Biolegend) for gating of MEMs. CD14 positive/pH pHrodo Green positive cells at Ex/Em of 509/533 nm were detected on the MACSQuant analyzer 10 (Miltenyi Biotec) and analyzed using FlowJo software (FlowJo, Inc, Ashland, OR).

Kink J.A., Forsberg M.H., Reshetylo S., Besharat S., Childs C.J., Pederson J.D., Gendron-Fitzpatrick A., Graham M., Bates P.D., Schmuck E.G., Raval A., Hematti P, & Capitini C.M. (2019). Macrophages Educated with Exosomes from Primed Mesenchymal Stem Cells Treat Acute Radiation Syndrome by Promoting Hematopoietic Recovery. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 25(11), 2124-2133.

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