Rabbit monoclonal anti-FOXO1, rabbit monoclonal anti-phospho-FOXO1 (Ser256), rabbit monoclonal anti-phospho-FOXO4 (Ser193), rabbit monoclonal anti-FOXO4, rabbit monoclonal anti-FOXO3, rabbit monoclonal anti-phospho-FOXO3a (Ser253), rabbit monoclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), rabbit monoclonal anti-JNK, rabbit monoclonal anti-phospho-JNK (Thr183/Tyr185) and Signal Silence FOXO1 siRNA II were from Cell Signaling Technology. JNK siRNA was from Sigma-Aldrich. Cell lysis buffer was from Cell Signaling. Alexa Fluor 488-conjugated anti-rabbit secondary antibodies, Texas Red phalloidin and Lipofectamine 2000 transfection agent were from Life Technologies. ProLong Gold was from Invitrogen. Horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse IgG and goat anti-rabbit IgG) were from Cell Signaling. ECL Western Blotting detection reagents were from Thermo Pierce. Tumour necrosis factor (PeproTech), NAC (Sigma-Aldrich), SP600125 (Cell Signaling) and insulin-like growth factor I (Sigma-Aldrich) were used for cell stimulation.
Rabbit monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit monoclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a primary antibody that recognizes the GAPDH protein, a key enzyme involved in glycolysis.
Automatically generated - may contain errors
Lab products found in correlation
Texas red phalloidin, by Thermo Fisher Scientific (1 mentions)
Prolong gold, by Thermo Fisher Scientific (1 mentions)
Cell lysis buffer, by Cell Signaling Technology (1 mentions)
N acetyl cysteine (nac), by Merck Group (1 mentions)
Lipofectamine 2000 transfection agent, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 conjugated anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions)
Rabbit monoclonal anti jnk, by Cell Signaling Technology (1 mentions)
Sp600125, by Cell Signaling Technology (1 mentions)
Jnk sirna, by Merck Group (1 mentions)
Mouse monoclonal anti β actin, by Merck Group (1 mentions)
2 protocols using rabbit monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh
1
Signaling pathway analysis in cells
2
Quantitative Western Blot Analysis
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Western blot analyses were performed as previously described [19 (link)]. The following primary Abs were used: mouse monoclonal anti-β actin (1:10,000; Sigma-Aldrich, MO, US), rabbit monoclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000; Cell Signaling Technologies, CA, USA), rabbit polyclonal anti-CX3CR1 (1:500; Abcam, Cambridge, UK), and mouse monoclonal anti-postsynaptic density-95 (PSD-95) (1:200, Abcam). Secondary Abs used were either horseradish peroxidase-conjugated anti-mouse or anti-rabbit (both 1:5000; Sigma-Aldrich). Band intensities were quantified using ImageJ software (NIH, USA), and β-actin or GAPDH was used as a loading control.
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