Glucose was measured at 1300–1400 h after fasting for 6 h with an Accu-Check Advantage meter (Roche Diagnostics, Sydney, Australia) in tail vein blood at different times after inoculation or in blood obtained via cardiac puncture at the time of sacrifice. Mice were considered diabetic if blood glucose was confirmed as ≥ 12.2 mM (mean + 3SD of 280 samples from C57/B6 mice over days 1–14 after mock inoculation). Plasma insulin was measured by enzyme-linked immunosorbent assay (ELISA) (Mercodia, Uppsala, Sweden) in the same tail vein blood sample.
Accu check advantage meter
Manufactured by Roche
Sourced in Australia, United Kingdom
The Accu-Chek Advantage meter is a portable blood glucose monitoring device used to measure the level of glucose in a person's blood. It is designed to provide users with quick and accurate readings to help manage their diabetes or monitor their blood sugar levels.
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Lab products found in correlation
2 protocols using accu check advantage meter
1
Measuring Diabetic Glycemia in Mice
2
Intraperitoneal Insulin Tolerance Test in Mice
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For intraperitoneal insulin tolerance tests (IPITT), mice were fasted for 3-4 hours before administering insulin. Insulin was prepared at 100 iu/ml in normal saline and used to resuspend nitrogen-dried 2-[1,2-3 H(N)]-deoxy-D-glucose (0.37 MBq). Basal blood glucose was measured in tail blood with an Accu-Check Advantage meter (Roche Diagnostics, Burgess Hill, West Sussex, UK). A further 10 µl of blood was collected in microfuge tube containing 1 iu heparin in saline, mixed and placed on ice. Immediately afterwards, insulin and deoxyglucose tracer was administered intraperitoneally at a dose of 0.75 iu/kg. At 15, 30, 60 and 90 minutes after insulin administration, blood glucose was measured as described above. After taking the final blood sample, the mice were euthanized by cervical dislocation and tibialis cranialis (TC), extensor digitorum longus, soleus muscles and epididymal fat pads were collected, weighed and frozen in liquid nitrogen-cooled isopentane.
Intraperitoneal glucose tolerance test (IPGTT) was conducted and glucose clearance into TC muscle was determined as described previously (7) .
Intraperitoneal glucose tolerance test (IPGTT) was conducted and glucose clearance into TC muscle was determined as described previously (7) .
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