Red cell distribution width was measured in blood samples collected in EDTA tubes, which were analyzed with an automated hematology analysis system (Mindray BC5800). Normal RDW values ranged from 11% to 16% in our laboratory. Standard laboratory parameters, including total leukocyte and neutrophil counts, hematocrit, glucose and creatinine levels and lipid profiles, were determined with standard methods. Plasma NT-proBNP levels were measured using a Cobas e411 device (Roche, Germany) via the immunoassay method. The NT-proBNP reference values were determined as 0–125 pg/ml. Hs-CRP was measured using a BN2 model nephelometer.
Cobas e411 device
Manufactured by Roche
Sourced in Germany
The Cobas E411 is an automated analyzer device designed for in vitro diagnostic testing. It is capable of performing electrochemiluminescence immunoassay (ECLIA) analysis on a variety of samples. The core function of the Cobas E411 is to automate the process of sample analysis and result generation for clinical chemistry and immunology testing.
Automatically generated - may contain errors
5 protocols using cobas e411 device
1
Comprehensive Blood Biomarkers Analysis
2
Serum PCT Levels in ICU Patients
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Serum PCT levels were measured with the electrochemiluminescence immunoassay method with a Roche Cobas E411 device. The PCT levels for patients were recorded on the TTM reporting forms on admission to ICU and in 24 and 48 hours were also included in the assessment.
3
Polycystic Ovarian Morphology in Early Follicular Phase
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In the early follicular phase (days 3–5) of spontaneous bleeding or withdrawal bleeding induced by medroxyprogesterone acetate, laboratory assessments and ultrasound sonographies were performed.
All participants underwent abdominal ultrasonography by a sonologist; ovaries containing 12 or more follicles measuring 2–9 mm in diameter and/or enlarged ovarian volume (> 10 mm3) were considered as a positive polycystic sonographic morphology [6 (link)].
All blood samples’ test was done after 8-h fasting and overnight bed rest in Arash Hospital laboratory. The laboratory investigation consisted of Sex Hormone-Binding Globulin (SHBG), Total Testosterone (TT) levels, and Free Androgen Index (FAI) which were computed by TT (nmol/L)/SHBG (nmol/L) × 100). Hyper androgenism was defined as TT greater than 0.68 ng/ml was greater than 5.36%. All of the above tests were assessed by commercial kits (Pars Azmoon Inc., Tehran, Iran) using Auto-analyzer BT2000 device. Biochemical measurement of TT and SHBG levels was carried out using Cobas E411 device based on electro-chemiluminescence method (Roche Instr Kit, Germany).
All participants underwent abdominal ultrasonography by a sonologist; ovaries containing 12 or more follicles measuring 2–9 mm in diameter and/or enlarged ovarian volume (> 10 mm3) were considered as a positive polycystic sonographic morphology [6 (link)].
All blood samples’ test was done after 8-h fasting and overnight bed rest in Arash Hospital laboratory. The laboratory investigation consisted of Sex Hormone-Binding Globulin (SHBG), Total Testosterone (TT) levels, and Free Androgen Index (FAI) which were computed by TT (nmol/L)/SHBG (nmol/L) × 100). Hyper androgenism was defined as TT greater than 0.68 ng/ml was greater than 5.36%. All of the above tests were assessed by commercial kits (Pars Azmoon Inc., Tehran, Iran) using Auto-analyzer BT2000 device. Biochemical measurement of TT and SHBG levels was carried out using Cobas E411 device based on electro-chemiluminescence method (Roche Instr Kit, Germany).
4
Serum Biomarker Measurement Protocol
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Serum PCT levels were measured with the electrochemiluminescence immunoassay method using a Roche Cobas E411 device at a reference range of 0.04–0.1 µg/L. Serum CRP levels were assayed via the nephelometric method using the Beckman Array 360 System at a reference range of 0–5 mg/L. The WBC count was determined using a hematological cell counter (LH 780 Analyzer, Beckman Coulter Inc., Miami, FL, USA) at a reference range of 4.2–10.6 × 103/µL.
5
sFlt-1 and PlGF Biomarkers Quantification
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The measurements of angiogenesis-related factors sFlt-1 and PlGF in peripherical blood were performed in the Roche Cobas e411 device (ROCHE®), with automated Elecsys® sFlt-1/PlGF kits (ROCHE®), using 50 μL of maternal serum, through immunoassays for the quantitative determination of such biomarkers based on electrochemiluminescence technology. The protocol was implemented according to the manufacturer instructions. The concentration results obtained by the assay were expressed in pg of the analyte per mL of serum - pg/mL. The values obtained were also used for the calculation of sFlt-1/PlGF ratio. The sFlt-1/PlGF ratio had a threshold implemented of 38 for statistical analysis.
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